A5075027812- Primate Behavior and Ecology
- Wildlife Ecology and Conservation
- Identification and Quantification in Food
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Amphibian and Reptile Biology
- Livestock and Poultry Management
- Animal Vocal Communication and Behavior
- Mosquito-borne diseases and control
- Livestock Farming and Management
- Malaria Research and Control
- interferon and immune responses
- Viral Infections and Vectors
- Genetic and phenotypic traits in livestock
- MicroRNA in disease regulation
- Chemokine receptors and signaling
- Animal Behavior and Reproduction
- Bat Biology and Ecology Studies
- Virology and Viral Diseases
- Forensic and Genetic Research
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Rabies epidemiology and control
- SARS-CoV-2 and COVID-19 Research
- Environmental DNA in Biodiversity Studies
Universiti Malaysia Sarawak
2013-2024
Sabah Wildlife Department
2014-2022
ORCID
2021
Ministry of Natural Resources and Environment
2008-2020
Academy of Sciences Malaysia
2020
Universiti Malaysia Terengganu
2018-2019
University of Malaya
2019
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata
2019
University of Teramo
2019
Hospital Sungai Buloh
2019
Pangolins, unique mammals with scales over most of their body, no teeth, poor vision, and an acute olfactory system, comprise the only placental order (Pholidota) without a whole-genome map. To investigate pangolin biology evolution, we developed genome assemblies Malayan ( Manis javanica ) Chinese M. pentadactyla pangolins. Strikingly, found that interferon epsilon IFNE ), exclusively expressed in epithelial cells important skin mucosal immunity, is pseudogenized all African Asian species...
The legal and illegal trade in wildlife for food, medicine other products is a globally significant threat to biodiversity that also responsible the emergence of pathogens threaten human livestock health our global economy. Trade likely played role origin COVID-19, viruses closely related SARS-CoV-2 have been identified bats pangolins, both traded widely. To investigate possible pangolins as source potential zoonoses, we collected throat rectal swabs from 334 Sunda (Manis javanica)...
Abstract The Sumatran rhinoceros Dicerorhinus sumatrensis is on the brink of extinction. Although habitat loss and poaching were reasons decline, today's reproductive isolation main threat to survival species. Genetic studies have played an important role in identifying conservation priorities, including for rhinoceroses. However, a species such as rhinoceros, where time essence preventing extinction, what extent should genetic geographical distances be taken into account deciding most...
The ex-situ propagation of the Malayan tapir in Malaysia is an important conservation strategy to increase population this endangered species. However, scarcity reproductive physiology studies impedes potential efforts. This study aimed investigate expression estrus signs through observation behaviour and vulva score captive tapirs. was conducted from January April at Sungai Dusun Wildlife Conservation Centre, Selangor, Malaysia. A total five female tapirs aged between 3 18 years old were...
The members of the genus Muntiacus are particular interest to evolutionary biologists due their extreme chromosomal rearrangements and ongoing discussions about number living species. Red muntjacs have largest distribution all were formerly considered as one Karyotype differences led provisional split between Southern Muntjac (Muntiacus muntjak) Northern (M. vaginalis), but uncertainties remain as, so far, no phylogenetic study has been conducted. Here, we analysed whole mitochondrial...
Abstract Multilocus microsatellite genotyping of Plasmodium knowlesi isolates previously indicated 2 divergent parasite subpopulations in humans on the island Borneo, each associated with a different macaque reservoir host species. Geographic divergence was also apparent, and independent sequence data have particularly deep between parasites from mainland Southeast Asia Borneo. To resolve overall population structure, multilocus conducted new sample 182 P. infections (obtained 134 48 wild...
The phylogenetic relationships of long-tailed macaque ( Macaca fascicularis ) populations distributed in Peninsular Malaysia relation to other regions remain unknown. aim this study was reveal the phylogeography and population genetics Malaysia’s M. f. based on D-loop region mitochondrial DNA. Sixty-five haplotypes were detected all populations, with only Vietnam Cambodia sharing four haplotypes. minimum-spanning network projected a distant relationship between Malaysian insular populations....
Phylogenetic relationships among Malaysia’s long-tailed macaques have yet to be established, despite abundant genetic studies of the species worldwide. The aims this study are examine phylogenetic Macaca fascicularis in Malaysia and test its classification as a morphological subspecies. A total 25 samples M. yielding 383 bp Cytochrome b (Cyt b) sequences were used analysis along with one sample each nemestrina arctoides outgroups. Sequence character reveals that Cyt locus is highly...
Abstract Pangolins are scale-covered mammals, containing eight endangered species. Maintaining pangolins in captivity is a significant challenge, part because little known about their genetics. Here we provide the first large-scale sequencing of critically Manis javanica transcriptomes from different organs using Illumina HiSeq technology, yielding ~75 Giga bases and 89,754 unigenes. We found some unigenes involved insect hormone biosynthesis pathway also 747 lipids metabolism-related that...
West Nile virus (WNV) is a zoonotic mosquito-borne flavivirus that harbored and amplified by wild birds via the enzootic transmission cycle. Wide range of hosts are found to be susceptible WNV infection including mammals, amphibians reptiles across world. Several studies have demonstrated was present in Malaysian Orang Asli captive birds. However, no data available on prevalence Malaysia. Therefore this study conducted determine serological molecular selected areas Coast Peninsular Two types...
Pangolins are the most trafficked mammal in world, and all eight species listed under CITES Appendix I. DNA-based wildlife forensic techniques recognized as an important component of investigating a pangolin seizure. In particular, determining seizure will 1) confirm presence to establish legality any trade, 2) ensure appropriate laws applied their fullest extent prosecution. Furthermore, valuable intelligence data, such geographic provenance samples, can be produced through analysis...
Abstract The legal and illegal trade in wildlife for food, medicine other products is a globally significant threat to biodiversity that also responsible the emergence of pathogens threaten human livestock health our global economy. Trade likely played role origin COVID-19, viruses closely related SARS-CoV-2 have been identified bats pangolins, both traded widely. To investigate possible pangolins as source potential zoonoses, we collected throat rectal swabs from 334 Sunda ( Manis javanica...
The illegal ivory trade continues to drive elephant poaching.Large seizures in Africa and Asia are still commonplace.Wildlife forensics is recognised as a key enforcement tool combat this trade.However, the time resources required effectively test large often prohibitive.This limits or delays testing, which may impede investigations and/or prosecutions.Typically, DNA analysis of an seizure involves pairing sorting tusks, sampling powdering sample, decalcification, then extraction.Here, we...
Abstract Background We analyzed a combined segment (2032-bp) of the sex-determining region and testis-specific protein Y-chromosome (Y-DNA) gene to clarify flow phylogenetic relationships long-tailed macaques ( Macaca fascicularis ) in Southeast Asia. Phylogenetic were constructed using maximum likelihood, Bayesian inference, median-joining network from total 164 adult male M. 62 localities Malaysia, including sequences other regions previous studies. Results Based on Y-DNA, we confirm...
The role of wildlife such as wild birds, macaques, and bats in the spreading maintenance deadly zoonotic pathogens nature have been well documented many parts world. One pathogen is mosquitoes borne virus, namely West Nile Virus (WNV). Previous research has shown that 1:7 1:6 Malaysian birds are WNV antibody RNA positive, respectively, North America may not be susceptible to infection. This study was conducted determine status macaques found mangrove forests caves, respectively. Archive sera...
Here, we present the first complete mitochondrial genome of Malayan Gaur (Bos gaurus hubbacki) inferred using next-generation sequencing. The mitogenome is 16,367 bp in length with structural organization a typical bovine arrangement comprising 13 protein-coding genes, 21 tRNAs, and 2 rRNAs. No internal stop codon was found genes. Phylogenetic tree analysis revealed that gaur more closely related to Burmese banteng instead gaur.
The cynomolgus macaque (Macaca fascicularis) is an extensively utilised nonhuman primate model for biomedical research due to its biological, behavioural, and genetic similarities humans. Genomic information of vital in various fields; however, there presently a shortage genomic on the Malaysian macaque. This study aimed sequence, assemble, annotate, profile Peninsular transcriptome derived from three tissues (lymph node, spleen, thymus) using RNA sequencing (RNA-Seq) technology. A total...
To demonstrate a noninvasive large mammalian genetic sampling method using blood meal obtained from tabanid fly.Blood was recovered the abdomen of an engorged fly (Haematopota sp.) which captured immediately after biting Sumatran rhino in captivity. The applied on to Whatman FTA(®) card. Subsequent laboratory work conducted extract, amplify and sequence DNA sample. Validation done by hair follicles samples rhinoceros subjecting it same process.BLAST search constructed phylogenetic trees...