The pathogen cereal cyst nematode (CCN) is deleterious to Triticeae crops and a threat the global crop yield. Accession no. 1 of Aegilops variabilis, relative Triticum aestivum (bread wheat), highly resistant CCN. Our previous study demonstrated that expression phenylalanine ammonia lyase (PAL) gene AevPAL1 in Ae. variabilis strongly induced by PAL, first enzyme phenylpropanoid metabolism, involved abiotic biotic stress responses. However, its role plant-CCN interaction remains unknown. In...

Bletilla striata is an endangered traditional Chinese medicinal plant with multiple uses and a slow regeneration rate of its germplasm resources. To evaluate the callus growth kinetics accumulation secondary metabolites (SMs), suspension culture was proven to be valuable approach for acquiring high yields compounds. An effective obtaining B. SMs achieved in vitro induction calluses from seeds. The were analyzed using mathematical model. resulting kinetic model revealed that curves...

Signaling molecules act as the links and bridges between endophytes host plants. The recognition of plants, regulation plant growth development, synthesis secondary metabolites are not separated by participation signaling molecules. In this review, we summarized types characteristics in medicinal plants recent processes intracellular conduction multi-molecular crosstalk during interactions endophytic bacteria addition, overviewed molecular mechanism signals medical metabolite accumulation...

Yield reliability under diverse environments is important to address climate stress consequences in wheat production systems. Breeding for a changing remains challenge wheat. We assessed the performance of 18 hexaploid ( Triticum aestivum L.) genotypes three field at location within semi-arid Canadian Prairies over four years with primary aim establish knowledge phenotypic plasticity and yield parental lines as it relates heat drought tolerance. collected data on various physiological traits...

The cultivation medium of Dendrobium nobile has an effect on the contents its main medicinal components, but specific mechanism is still unclear. In this study, callus, seedlings, rhizomes, and leaves D. were sequenced for PacBio SMRT. 2-year-old stems selected Illumina sequencing metabolome to analyze genetic metabolic differences under different epiphytic patterns. As a result, total 387 differential genes obtained, corresponding 66 metabolites. Different patterns can induce series changes...

Context: Endophytic fungi are microorganisms living within the tissues of host plants, and have proven to be rich sources biologically active secondary metabolites therefore attracted increasing attention in recent years.Objective: To isolate characterize bioactive constituents from endophytic fungus cultures Crocus sativus Linn. (Iridaceae).Materials methods: Endophytes were isolated corm C. sativus. subjected repeated column chromatography. Chemical structure was elucidated based on...

Bletilla striata has been widely used in the pharmacology industry. To effectively produce secondary metabolites through suspension cultured cells of B. striata, it is important to exploring full-length transcriptome data and genes related cell growth chemical producing all culture stages. We applied a combination Real-Time Sequencing Single Molecule (SMRT) second-generation sequencing (SGS) generate complete cells.The was formed de novo way by using PacBio isoform (Iso-Seq) on pooled RNA...

Dendrobium nobile , an epiphytic plant, is a traditional medicinal herb with abundant endophytes. It unclear whether the variation in diversity and abundance of endophytes could stimulate biosynthesis compounds plant. In this study, we collected fresh stems D. from four habitats for investigating fungal community structure, dendrobine content, environment factors their correlations. The results indicated no significant difference endophytic among habitats; however, different dominant or...

To screen the genes regulating biosynthesis of phenolic acid derivatives from genome Bletilla striata , we designed a suspension culture system to sample cells for following experiments. The contents four were determined by high-performance liquid chromatography, and several full-length transcriptome sequencings RNA samples at 10 time points performed bioinformatics analysis. correlation analysis was used identify verify key DEGs involved in derivatives. results showed that...

Bletilla striata is an endangered orchid that has been used for millennia as a medicinal herb, in cosmetics and horticultural plant. To construct the first nucleotide database this species to develop abundant EST-SSR markers facilitating further studies, various tissues organs of plants main developmental stages were harvested mRNA isolation subsequent RNA sequencing. A total 106,054,784 clean reads generated by using Illumina paired-end sequencing technology. The assembled into 127,261...

Cereal cyst nematode (CCN, Heterodera avenae) is a most important pathogen of wheat and causes tremendous yield loss annually over the world. Since lack resistance materials among cultivars, identification characterization resistance-related genes from relatives necessary efficient way. As close relative with high against CCN, Aegilops variabilis No.1 believed to be valuable source for breeding this devastating disease. However so far, very few resistance-associated have been characterized...

A new triphenanthrene compound named 2,2′,2′′,7,7′,7′′-hexahydroxy-4,4′,4′′-trimethoxy-[9,9′,9′′,10,10′,10′′]-hexahydro-1,8,1′,6′′-triphenanthrene (1), together with eleven known compounds (2-12), were isolated from tubers of Bletilla striata. Their structures determined by analysis spectroscopic data.

Auxin/Indole-3-Acetic Acid (Aux/IAA) genes are involved in auxin signaling pathway and play an important role plant growth development. However, many studies focus on Aux/IAA gene families much less known Bletilla striata. In this study, a total of 27 (BsIAA1-27) were cloned from the transcriptome Based phylogenetic analysis protein sequences B. striata, Arabidopsis thaliana Dendrobium officinale, striata (BsIAAs) categorized into 2 subfamilies 9 groups. While BsIAAs more closer to those D....

Paphiopedilum armeniacum is a rare orchid native to China with high ornamental value. The germination of P. seeds difficult, especially for the mature seeds, which major limitation their large-scale reproduction. This study explored reasons seed inhibition from aspects important plant endogenous hormone—abscisic acid (ABA). hormone contents were determined at different developmental stages. ABA content was 5.8 ng/g in 73 days after pollination (DAP) immature peaked 14.6 129 DAP and dropped...

Abstract Coridius chinensis , a valued species used in traditional Chinese medicine, undergo overwintering diapause. To clarify the mechanisms, we examined how low temperature treatments can affect diapause and cold tolerance C. . Using transcriptome analysis, obtained differently expressed 3225 mRNAs (DEGs) 177 long non-coding RNAs (lncRNAs). The more down-regulated DEGs lncRNAs groups (DP) than low-temperature (LT) stress when compared with wild control (WC) groups. From co-expression...

Agaricus bisporus is a delicious, nutritious mushroom with medical function. Cross breeding limited due to its taxonomic contradiction. We analyzed rDNA ITS sequences of two high quality strains (No.Tb07, As2796). Tb07 strain was selected from foreign by CSUFT under protection. As2796 local in nature China. By sequencing the regions and comparing those Genebank BLAST search, results concluded that region length 701 bp 695 bp. GC content ranged 41.72% 42.22% ITS1 43.89% 44.31% ITS2 region....

Abstract Bletilla striata (Orchidaceae) is a well-recognized endangered medicinal plant due to inadequate natural reproduction with high market worth. To evaluate the cell growth kinetics and accumulation of secondary metabolites (SMs), suspension culture proved be valuable approach for acquiring yield parts. An effective obtaining B. its SMs was in vitro induction callus from seeds. The were analyzed using mathematical model. Results kinetic model revealed that curve cells curved as sigmoid...

Abstract A0709‐BX05 (Reg. no. GP‐1060, PI 698311) is a Canada western amber durum (CWAD) derived wheat [ Triticum turgidum L. subsp. (Desf.) Husn.] line developed at the Swift Current Research and Development Centre, Agriculture Agri‐Food Canada. was selected by modified pedigree breeding procedure from single cross of A0262&AM050/‘Strongfield’ made in 2007. evaluated for ergot resistance based on honeydew sclerotial production spikes greenhouse testing 2012 to 2018. expresses highly...