A5020633997- Oral microbiology and periodontitis research
- Endodontics and Root Canal Treatments
- Dental materials and restorations
- Antimicrobial Peptides and Activities
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Dental Radiography and Imaging
- Probiotics and Fermented Foods
- Dental Implant Techniques and Outcomes
- Bone and Dental Protein Studies
- Mesenchymal stem cell research
- Infective Endocarditis Diagnosis and Management
- Dental Health and Care Utilization
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Dental Anxiety and Anesthesia Techniques
- Single-cell and spatial transcriptomics
- Antimicrobial Resistance in Staphylococcus
- Dental Trauma and Treatments
- Bone Metabolism and Diseases
- Nosocomial Infections in ICU
- Dental Research and COVID-19
- Biochemical and Structural Characterization
- CRISPR and Genetic Engineering
Sun Yat-sen University
2014-2024
Hospital of Stomatology, Sun Yat-sen University
2015-2024
Stomatology Hospital
2015-2022
Key Laboratory of Guangdong Province
2017-2018
Air Force Medical University
2008-2012
Abstract Targeted delivery of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system to the receptor cells is essential for in vivo gene editing. Exosomes are intensively studied as a promising targeted drug carrier recently, while limited by their low efficiency encapsulating large nucleic acids. Here, kind hybrid exosomes with liposomes developed via simple incubation. Different from original exosomes, resultant nanoparticles...
Enterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic acquired resistance to a variety antibiotics. Development new antibiotics is limited, pathogens continually generate antibiotic resistance. Many researchers aim identify strategies effectively kill this drug-resistant pathogen. Here, we evaluated effect antimicrobial peptide nisin on antibacterial activities 18 against E. faecalis. The MIC MBC results showed that OG1RF, ATCC...
Age related defect of the osteogenic differentiation mesenchymal stem cells (MSCs) plays a key role in osteoporosis. Mechanical loading is one most important physical stimuli for osteoblast differentiation. Here, we compared potential MSCs from young and adult rats under three rounds 2 h cyclic stretch 2.5% elongation at 1 Hz on 3 consecutive days. Cyclic induced significant rats, while compromised osteogenesis rats. Accordingly, there were much more reactive oxygen species (ROS) production...
Free D-amino acids (D-AAs) are one of the most striking features peptidoglycan composition in bacteria and play a key role regulating disassembling bacterial biofilms. Previous studies have indicated that antimicrobial peptide nisin can inhibit growth cariogenic Streptococcus mutans. The present study investigated effect free amino either alone or combination with on biofilm planktonic S. mutans bacteria. results MIC MBC analyses showed D-cysteine (Cys), D- L-aspartic acid (Asp), L-glutamic...
The aim of this study was to evaluate the effects butt margin, occlusal thickness and pulp chamber extension depth on stress distributions mandibular molar endodontically treated teeth (ETT) with EMAX endocrown restoration using 3-dimensional finite element analysis (FEA). FEA models flat surface or curve margin were firstly evaluated distributions, then 9 1-, 2- 3-mm generated margin. In all models, a 200 N vertical load horizontal applied, von Mises (VMS) evaluated. results showed that...
Chronic apical periodontitis (CAP) is a common infectious disease of the oral cavity. Immune responses and osteoclastogenesis monocytes/macrophages play crucial role in CAP progression, this study want to clarify CAP, which will contribute treatment CAP. We aim explore heterogeneity monocyte populations periapical lesion tissues healthy control (HC) periodontal by single-cell RNA sequencing (scRNA-seq), search novel targets for alleviating further validate it proteomics vitro vivo...
Abstract The antibacterial properties of resins incorporating MAE‐DB and the underlying mechanisms action were evaluated. Antibacterial effects against Streptococcus mutans tested using film contact method, with accumulation membrane integrity observed by scanning electron microscopy confocal laser microscopy, respectively. Quantitative PCR was used to determine expression S. glucosyltransferase B ( gtfB ) gene on surface containing 10% MAE‐DB. Bacterial growth inhibited resin as compared...
Periodontitis, a common disease that can lead to bone destruction, periodontal attachment loss, and tooth is the major cause for oral tissue engineering. Experimental periodontitis suitable disease-model studying regeneration potential therapeutic role of biomaterials on engineering, as this in vivo model could be employed mimic natural host response under bacteria-caused pathological environments. Although large animals with ligature-induced have mostly been used experiments, mouse better...
To investigate the effects of two Enterococcus faecalis root canal isolated strains (CA1 and CA2) OG1RF strain on apoptosis, pyroptosis, necroptosis in macrophages. The virulence factors E. CA1 CA2 pathogenic were annotated Virulence Factors Database (VFDB). CA1, CA2, used to infect RAW264.7 macrophages (MOI, 100:1). We assessed viability intracellular extracellular bacteria at 2, 6, 12 h post-infection. a live cell imaging analysis system obtain dynamic curve death after infection by each...
Persistent apical periodontitis (PAP) is characterized by refractory inflammation and progressive bone destruction. Enterococcus faecalis infection considered an important etiological factor for the development of PAP, although exact mechanisms remain unknown. This study aimed at investigating role E. in cell proliferation, inflammatory reactions osteoclast differentiation macrophages using vitro model precursor RAW264.7 cells. A viability assay cultured cells exposed to live a multiplicity...