A5101778923- Oral microbiology and periodontitis research
- Endodontics and Root Canal Treatments
- Pancreatitis Pathology and Treatment
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Infective Endocarditis Diagnosis and Management
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Phagocytosis and Immune Regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lipid metabolism and disorders
- Biomarkers in Disease Mechanisms
- Cerebrovascular and Carotid Artery Diseases
- Cardiac Fibrosis and Remodeling
- Nanoparticles: synthesis and applications
- Neonatal and Maternal Infections
- Electrospun Nanofibers in Biomedical Applications
- Phytochemistry and biological activity of medicinal plants
- RNA Interference and Gene Delivery
- Immune cells in cancer
- Dental Radiography and Imaging
- E-commerce and Technology Innovations
- Organoselenium and organotellurium chemistry
Sun Yat-sen University
2014-2025
Stomatology Hospital
2018-2025
West China Hospital of Sichuan University
2024-2025
Sichuan University
2024-2025
Hospital of Stomatology, Sun Yat-sen University
2018-2024
Shanghai Ninth People's Hospital
2014-2023
Kunming Medical University
2019-2021
Key Laboratory of Guangdong Province
2020
Academic Center for Dentistry Amsterdam
2016-2018
University of Amsterdam
2016-2018
Abstract Targeted delivery of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system to the receptor cells is essential for in vivo gene editing. Exosomes are intensively studied as a promising targeted drug carrier recently, while limited by their low efficiency encapsulating large nucleic acids. Here, kind hybrid exosomes with liposomes developed via simple incubation. Different from original exosomes, resultant nanoparticles...
Enterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic acquired resistance to a variety antibiotics. Development new antibiotics is limited, pathogens continually generate antibiotic resistance. Many researchers aim identify strategies effectively kill this drug-resistant pathogen. Here, we evaluated effect antimicrobial peptide nisin on antibacterial activities 18 against E. faecalis. The MIC MBC results showed that OG1RF, ATCC...
Alveolar bone resorption caused by bacteria‐induced periodontitis remains challenging due to sustained inflammation. Periodontal pathogens like Porphyromonas gingivalis launch the primed signal of NOD‐like receptor family pyrin domain‐containing 3 (NLRP3) inflammasome in macrophages; consequent overproduction proinflammatory cytokines and reactive oxygen species (ROS) leads tissue destruction. This provides potential targets for a new therapeutic strategy. Herein, multifunctionalized...
Free D-amino acids (D-AAs) are one of the most striking features peptidoglycan composition in bacteria and play a key role regulating disassembling bacterial biofilms. Previous studies have indicated that antimicrobial peptide nisin can inhibit growth cariogenic Streptococcus mutans. The present study investigated effect free amino either alone or combination with on biofilm planktonic S. mutans bacteria. results MIC MBC analyses showed D-cysteine (Cys), D- L-aspartic acid (Asp), L-glutamic...
Periodontitis, a common disease that can lead to bone destruction, periodontal attachment loss, and tooth is the major cause for oral tissue engineering. Experimental periodontitis suitable disease-model studying regeneration potential therapeutic role of biomaterials on engineering, as this in vivo model could be employed mimic natural host response under bacteria-caused pathological environments. Although large animals with ligature-induced have mostly been used experiments, mouse better...
Whether circulating histones in gut lymph contribute to organ failure and impact of chaiqin chengqi decoction (CQCQD) on severe acute pancreatitis (SAP) remain elusive. To verify the role SAP evaluate effect CQCQD them. Sodium taurocholate was retrogradely infused into pancreatobiliary duct induce rodents. Various regimens were administered intragastrically or via duodenum followed by dynamic collection rats. The endothelial cell viability lymphocytes determined. Components identified...
In recent years, due to the drug resistance and side effects caused by microbe infection modification, people have begun implement strategies reduce prevent infection. Therefore, a synergistic strategy is designed prepare bioactive polypropylene/silver phosphate (PP/Ag 3 PO 4 ) composites with different Ag contents combining PP through melt blending, micrometer‐scale surfaces inspired lotus leaves are fabricated using hot embossing technique. By comparing analyzing obtained data, it found...
Therapeutic angiogenesis by transplantation of autologous/allogeneic adipose-derived stem cells (ADSCs) is a potential approach for severe ischemic diseases. However, poor viability, adhesion, migration and differentiation limit the therapeutic efficiency after were transplanted into targeted area. Periostin, an extracellular matrix protein, exhibits critical role in wound repair as well promotes cell survival, angiogenesis.ADSCs obtained genetically engineered with periostin gene (P-ADSCs)....
Exposure to antibiotics is considered be the major driver in selection of antibiotic-resistant bacteria and may induce diverse biological responses bacteria. MTAD a common intracanal irrigant, but its bactericidal activity remains improved. Previous studies have indicated that antimicrobial peptide nisin can significantly improve against Enterococcus faecalis. However, effects modification at sub-minimum inhibitory concentration (sub-MIC) levels on faecalis growth expression pathogenic genes...
Human dentin, as an important calcified tissue in the body, plays significant roles withstanding masticatory forces and has a complex hierarchical organization. Understanding composition ultrastructure of dentin is critical for elucidating mechanisms biomineralization under healthy pathological states. Here, atomic force microscope infrared spectroscopy (AFM-IR) AFM-based amplitude modulation-frequency modulation (AM-FM) techniques were utilized to detect heterogeneity chemical mechanical...
A high prevalence of Enterococcus faecalis (E. faecalis) is observed in teeth with root canal treatment failures. Clustered regularly interspaced short palindromic repeats (CRISPR) are widely distributed prokaryotes that have adaptive immune systems against mobile elements, including pathogenic genes. The present study investigated the relevance CRISPR E. strains isolated from retreated canals on biofilms, periapical lesions and drug resistance. total 20 were extracted referred for...
Bacteria in nature are present different lifestyles with distinct characteristics. Streptococcus mutans is the etiologic pathogen of dental caries and could easily gain access into bloodstream after oral surgery adopt a biofilm lifestyle, resulting infective endocarditis. A growing amount evidence have revealed that large web-like structure composed extracellular DNA antimicrobial proteins released by neutrophils, named Neutrophil Extracellular Traps (NETs), play an active role defense...
Abstract Periodontitis is an exemplar of dysbiosis associated with the coordinated action multiple members within microbial consortium. The polymicrobial synergy and hypothesis proposes a dynamic host-microbiome balance, certain modulators capable disrupting eubiosis driving shifts towards community. However, these factors remain to be explored. We established Porphyromonas gingivalis- or Aggregatibacter actinomycetemcomitans-modified subgingival microbiome model 16S rRNA sequencing revealed...
Abstract Background Type 2 diabetes (T2DM) affects billions of patients and has a pathophysiologically important inflammatory component. Macrophage plasticity in is impaired, remains persistent proinflammatory state; yet currently, the cause state unknown. Phenylacetylglutamine (PAGln) an amino acid metabolism end product that rises T2DM associated with incident major adverse cardiovascular events. However, role PAGln component Purpose Here, we aim to identify blood-based systemic factors...
The present study aimed to evaluate the effects of Enterococcus faecalis, inactivated by common intracanal irrigants sodium hypochlorite (NaOCl) and chlorhexidine (CHX), on osteoblasts. E. faecalis was with 2% CHX or 5.25% NaOCl. Subsequently, Cell Counting kit‑8 assay used examine CHX‑ NaOCl-inactivated MC3T3‑E1 osteoblast cell proliferation. Alizarin red staining determine mineralization, osteogenic induction quantified determining optical density dye solution. relative expression levels...
Porphyromonas gingivalis is present as a biofilm at the sites of periodontal infections. The detachment gingival epithelial cells induced by P. biofilms was examined using planktonic cultures comparison. Exponentially grown or 40-h were co-incubated with in 24-well plate for 4 h. Epithelial cell assessed imaging. activity arginine-gingipain (Rgp) and gene expression profiles gingipain assay quantitative PCR, respectively. significantly higher displayed Rgp compared to cultures. genes...