A5051501141- Pancreatitis Pathology and Treatment
- Extracellular vesicles in disease
- Lymphatic Disorders and Treatments
- Liver Disease Diagnosis and Treatment
- Inflammatory Bowel Disease
- Lymphatic System and Diseases
- Bacterial Infections and Vaccines
- Lipid metabolism and disorders
- Amoebic Infections and Treatments
- Pancreatic and Hepatic Oncology Research
- Esophageal and GI Pathology
- Reproductive System and Pregnancy
- Inflammasome and immune disorders
- Peroxisome Proliferator-Activated Receptors
- Helicobacter pylori-related gastroenterology studies
- Tuberculosis Research and Epidemiology
- Gastroesophageal reflux and treatments
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Cerebrospinal fluid and hydrocephalus
- Immune Response and Inflammation
- Ion Transport and Channel Regulation
- Mycobacterium research and diagnosis
- Intensive Care Unit Cognitive Disorders
- dental development and anomalies
University of Auckland
2016-2025
Ajou University
2024
Gwangyang Health College
2024
Maurice Wilkins Centre
2019-2023
Case Western Reserve University
2023
Danylo Halytsky Lviv National Medical University
2022
University of Pennsylvania
2022
University of Pennsylvania Health System
2022
Seoul National University Dental Hospital
2019
Pusan National University Hospital
2016
Bacterium-to-host signalling during infection is a complex process involving proteins, lipids and other diffusible signals that manipulate host cell biology for pathogen survival. Bacteria also release membrane vesicles (MV) can carry cargo of effector molecules directly into cells. Supported by recent publications, we hypothesised these MVs associate with RNA, which may be involved in the modulation response to infection.Using uropathogenic Escherichia coli (UPEC) strain 536, have isolated...
Bacteria release nano-sized extracellular vesicles (EVs) into the milieu. Bacterial EVs contain molecular cargo originating from parent bacterium and have important roles in bacterial survival pathogenesis. Using 8-plex iTRAQ approaches, we profiled EV proteome of two Escherichia coli strains, uropathogenic (UPEC) 536 probiotic Nissle 1917. For these compared crude input prepared by ultracentrifugation alone with purified either density gradient centrifugation (DGC) or size exclusion...
Prokaryotes release membrane vesicles (MVs) with direct roles in disease pathogenesis. MVs are heterogeneous when isolated from bacterial cultures so Density Gradient Centrifugation (DGC) is valuable for separation of MV subgroups contaminating material. Here we report the technical variability and natural biological heterogeneity seen between DGC preparations Mycobacterium smegmatis Escherichia coli compare these data size exclusion chromatography (SEC) columns. Crude MVs, by...
Chaiqin chengqi decoction (CQCQD) is a Chinese herbal formula derived from dachengqi decoction. CQCQD has been used for the management of acute pancreatitis (AP) in West China Hospital more than 30 years. Although well-established clinical efficacy, little known about its bioactive ingredients, how they interact with different therapeutic targets and pathways to produce anti-inflammatory effects. Toll-like receptor 4 (TLR4) nucleotide-binding oligomerization domain-like family pyrin domain...
Drugs are commonly administered via the intraperitoneal (IP) route to treat localized infections and cancers in patients test drug efficacy toxicity preclinical studies. Despite this, there remain large gaps our understanding of absorption routes (lymph vs blood) pharmacokinetics following IP administration. This is particularly true when drugs complex delivery systems such as liposomes which main marketed formulation for several that intraperitoneally. study investigated impact liposome...
The intestinal lymphatic system transports fluid, immune cells, dietary lipids, and highly lipophilic drugs from the intestine to systemic circulation. These transport functions are important health when dysregulated contribute pathology. This has generated significant interest in approaches deliver lymphatics. Most of current understanding lymph flow, lipid drug rates, comes vitro studies vivo animal studies. In contrast, human subjects have been limited. Recently, three surgical patients...
Background/Aims: Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality worldwide. Despite identification several biomarkers for HCC diagnosis, challenges such as low sensitivity and intratumoral heterogeneity have impeded early detection, highlighting the need etiology-specific blood biomarkers.Methods: We generated whole-transcriptome sequencing (WTS) targeted proteome data from buffy coat plasma samples patients. By integrating etiological information on viral infection, we...
Whether circulating histones in gut lymph contribute to organ failure and impact of chaiqin chengqi decoction (CQCQD) on severe acute pancreatitis (SAP) remain elusive. To verify the role SAP evaluate effect CQCQD them. Sodium taurocholate was retrogradely infused into pancreatobiliary duct induce rodents. Various regimens were administered intragastrically or via duodenum followed by dynamic collection rats. The endothelial cell viability lymphocytes determined. Components identified...
Polymorphisms in the organic cation transporter (OCTN) genes SLC22A4 (OCTN1; polymorphism 1672C/T) and SLC22A5 (OCTN2; -207G/C) at inflammatory bowel disease (IBD) 5 locus comprise a two-allele haplotype (SLC22A-TC) associated with increased risk for Crohn's (CD). In this study, we examined contribution of susceptibility SLC22A-TC to CD New Zealand Caucasian population. The frequencies gene polymorphisms 1672C/T -207G/C were 182 patients 188 ethnically matched controls by PCR-RFLP analysis....
Chronic rhinosinusitis (CRS) is a heterogeneous condition characterized by persistent sinus inflammation and microbial dysbiosis. This study aimed to identify clinically relevant subgroups of CRS patients based on distinct signatures, with comparison the commonly used phenotypic subgrouping approach. The underlying drivers these clusters were also investigated, together associations epithelial barrier integrity. Sinus biopsy specimens collected from (n = 23) disease controls 8). expression...
Chaiqin chengqi decoction (CQCQD) and its derivatives have been widely used in China for the early management of patients with acute pancreatitis (AP). Numerous studies demonstrate anti-inflammatory anti-oxidative effects CQCQD derivatives, but whether these can be attributed to suppressing neurogenic inflammation, has never studied. To investigate on substance P (SP)-neurokinin 1 receptor (NK1R) based inflammation an experimental AP model. For admission, pain score was accessed by visual...
IntroductionIschemic gut injury is common in the intensive care unit, impairs barrier function, and contributes to multiorgan dysfunction. One novel intervention mitigate ischemic direct luminal delivery of oxygen microbubbles (OMB). Formulations OMB can be modified control rate delivery. This project examined whether pectin-modified (OMBp5) reduce a rodent model.MethodsThe OMBp5 formulation was adapted improve along length small intestine. Adult Sprague–Dawley rats (n = 24) were randomly...
Prostaglandins and endogenous cannabinoid metabolites share the same lipid backbone with differing polar head groups at exactly position through which a large molecule is attached to provide antigenicity thus raise antisera. Hence, we hypothesized that antisera raised against prostaglandins linked such as BSA carboxyl functional group would also recognize lead highly misleading interpretations of data. We found major cross-reactivity commercial endocannabinoid metabolites. Furthermore, in...
Abstract Trichomonas vaginalis is an extracellular protozoan parasite of the human urogenital tract, responsible for a prevalent sexually transmitted infection. Trichomoniasis accompanied by dysbiotic microbiome that characterised depletion host‐protective commensals such as Lactobacillus gasseri , and flourishing bacterial consortium comparable to one seen vaginosis, including founder species Gardnerella . These two vaginal bacteria are known have opposite effects on T. pathogenicity....