Protein motions are ubiquitous and intrinsically coupled to catalysis. Their specific roles, however, remain largely elusive. Dynamic loops at the active center of E1 component Escherichia coli pyruvate dehydrogenase multienzyme complex essential for several catalytic functions starting from a predecarboxylation event culminating in transfer acetyl moiety E2 component. Monitoring kinetics its loop variants various solution viscosities, we show that rate chemical step is modulated by...

The first component (E1o) of the Escherichia coli 2-oxoglutarate dehydrogenase complex (OGDHc) was engineered to accept substrates lacking 5-carboxylate group by subjecting H260 and H298 saturation mutagenesis. Apparently, is required for substrate recognition, but could be replaced with hydrophobic residues similar molecular volume. To interrogate whether second would allow synthesis acyl-coenzyme A derivatives, hybrid complexes consisting recombinant components OGDHc (o) pyruvate (p)...

The bacterial pyruvate dehydrogenase complex carries out conversion of to acetyl-coenzyme A with the assistance thiamin diphosphate (ThDP), several other cofactors, and three principal protein components, E1–E3, each present in multiple copies. E2 component forms core complexes, copy consisting variable numbers lipoyl domains (LDs, lipoic acid covalently amidated at a lysine residue), peripheral subunit binding (PSBDs), catalytic (or core) (CDs). reaction starts ThDP-dependent...

We used morphological and genetic data to assess the taxonomic status of Rana amurensis coreana R. a. amurensis. Morphological comparisons revealed these two subspecies be different from each other in size body, nature tubercles on dorsal stripe, degree development toe webbing, condition lateral spots trunk. They were also sequence mitochondrial 16S rRNA gene, with distance as large those observed among species brown frogs. Therefore, Korean populations previously considered a should...

Salmonella cells desiccated in an environment with low-water activity (aw ) show longer survival times and enhanced resistance to heat. However, little is known about the cellular ultrastructure of low-aw relation persistence during desiccation.In this study, Enteritidis strain PT30 was dehydrated by exposure air or mixing wheat flour 0·30 at room temperature) for 7 days followed heat treatment 80°C 10, 20, 60 min respectively. Transmission electron microscopy (TEM) employed examine compare...

Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen mice | Youngmin Park, Hyangju Kang, Kyungmin Min, Nam Hyung Kim, Minhee In-Ohk Ouh, Ha-Hyun Jae-Young Song, Dong-Kun Yang, Eun-Ju Sohn, Sangmin Lee Agricultural Journals

The objective of this study was to analyze the genetic diversity using SSR marker and investigate fatty acid composition perilla (P. frutescens var. frutescens) germplasm. Genetic among 95 accessions, which consisted 29 weedy types 66 landrace evaluated based on 12 markers carrying 91 alleles. mean values observed (<TEX>$H_O$</TEX>) expected heterozygosities (<TEX>$H_E$</TEX>) were 0.574 0.640, respectively, indicating a considerable amount polymorphism within collection. A distance-based...

A rapid and sensitive indirect competitive enzyme immunoassay method has been developed for quantitating ginsenoside Rf (Rf) in crude total Panax ginseng saponins rat plasma using high titer mouse monoclonal antibody (mAb) raised against a conjugate of bovine serum albumin (BSA). The isotype mAb was IgG3 with K chain. presence inhibited the binding anti-Rf to Rf-BSA solid phase coating antigen. working range 0.01-10 ng/assay detection limits were 20 pg various extract fractions or 34 per...

For the long-term preservation of genetic resources, cryopreservation techniques have been developed for strawberry germplasm, mainly using in vitro-grown shoot tips. In this study, stability was tested under greenhouse conditions six accessions (IT232511, PHS0132, IT245810, IT245830, IT245852, and IT245860) derived from following procedures: (1) conventional propagation (GH: maintained); (2) vitro (TC: tissue culture); (3) pretreatment before (-LN: non-liquid nitrogen exposure); (4) (+LN:...

Cryopreservation is a promising method for the long-term preservation of plant germplasm, especially vegetatively propagated species like freesias. In this study, we investigate streamlining cryopreservation process ‘Sunny Gold’ Freesia, starting from effective in vitro initiation and proliferation using various growth regulator combinations. We also assess impact subculture on regrowth rates after cryopreservation. The shoot tips were successfully initiated sterilization. shoots multiplied...

Extensive research has been conducted on the in vitro mass propagation of pear (Pyrus spp.) trees through vegetative propagation, demonstrating high efficiency shoot multiplication across various species. However, low rooting rates remain a significant barrier to practical application and commercialization propagation. This study aims determine favorable conditions for inducing root formation microshoots Pyrus genotypes. The base was exposed concentration (2 mg L−1) auxins (a combination IBA...

<title>Abstract</title> Stable isotopes provide knowledge of dynamic relationships within ecosystems. However, research on the trophic niche domestic anurans—which serve as biological indicator ecosystem health—using stable isotope analysis is limited. Therefore, we analyzed values in bone collagen three anuran species—<italic>Bombina orientalis</italic>, <italic>Rana uenoi</italic>, and <italic>R. huanrenensis</italic>—representative forest to determine levels, partitioning, overlap, shifts...

Abstract : Canine parvovirus (CPV) is a major diarrhea-causing agent in puppies. Since CPV type 2 (CPV-2) emergedin 1978, new antigenic variants including CPV-2a, CPV-2b, and CPV-2c have been identified many countries. Twopuppies died suddenly at veterinary clinic Gyeonggi province, South Korea. Two viruses were isolated A72cells, confirmed as strains based on rapid kit an indirect fluorescence test designated QIACP1403and QIACP1404. The nucleotide sequences of complete VP2 genes QIACP1403...

Abstract The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the regenerated plants three cultivars ( L . bolanderi ‘Lenora’, L. ‘Mount Duckling’ and Dragon’) one variety callosum var. flavum ) after cryopreservation, compared with fresh (donor) non-cryopreserved using morphological traits...

Forty one strains isolated from 21 samples of various mushroom composts in Korea were analyzed by 16S rRNA gene sequencing to investigate the phylogenetic diversity thermophilic actinomycetes. The 25 actinomycete isolates related five genera, Pseudonocardia, Saccharomonospora, Saccharopolyspora, Streptomyces, and Thermobifida, within order Actinomycetales, 16 classified into genus Thermoactinomyces family Bacillaceae. Most 41 encompassed two Streptomyces Thermoactinomyces, that mainly...

Sequence‐specific NMR assignments were made for six residues in the N‐terminal 1–55 region, and one each of inner (401–413) outer (541–557) active center loops E1 component E. coli pyruvate dehydrogenase complex (E1ec, 2x100 kDa), flexible regions not seen x‐ray structure. The 2D HSQC spectrum revealed 103 resonances 109 expected three regions. Site‐specific substitutions appropriate labeling patterns assisted assignments. Resonances assigned to region diminished size/were absent presence...

초파리는 유전학, 발달 생물학, 행동 노화 연구에 이르기까지 수많은 이용 되어져 왔다. 최근 초파리 전체 유전자의 염기서열이 보고되었으며, 기능 분석과 발현 단백질에 대한 연구가 진행되고 있다. 본 연구에서는 야생형 초파리에서 추출한 단백질을 이차원 전기영동을 통해 400여개 이상의 단백질 스폿으로 분리하였으며, 각 스폿을 적출하여 트립신으로 처리하여 얻어진 펩타이드 단편을 MALDI-TOF-MS를 이용한 지문 검색으로 질량을 측정하였다. 측정된 데이터 베이스를 이용하여 분리한 동정함으로써 59개의 유전자에서 발현되는 65개의 동정하였다. 이러한 결과는 향후의 발생단계, 외부 자극, 등에 관련되는 특이적 연구의 기초 자료로 활용될 수 있을 것이다. With the completely discovery of Drosophila genome sequence, next great challenge is to extract its biological information by...