A5052147729- Transgenic Plants and Applications
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Plant tissue culture and regeneration
- COVID-19 Clinical Research Studies
- Telomeres, Telomerase, and Senescence
- melanin and skin pigmentation
- Bacteriophages and microbial interactions
- Heat shock proteins research
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Skin Protection and Aging
- Toxin Mechanisms and Immunotoxins
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Microbial Inactivation Methods
- Enzyme Structure and Function
- Insect symbiosis and bacterial influences
CorSolutions (United States)
2024
Pohang TechnoPark (South Korea)
2018-2024
Pohang University of Science and Technology
2011-2019
Korea Basic Science Institute
2018
Chungnam National University
2018
Abstract Plastid proteins that are encoded by the nuclear genome and synthesized in cytosol undergo posttranslational targeting to plastids. Ankyrin repeat protein 2A (AKR2A) AKR2B were recently shown be involved of plastid outer envelope. However, it remains unknown whether other factors this process. In study, we investigated a factor AKR2A-mediated chloroplasts Arabidopsis (Arabidopsis thaliana). Hsp17.8, member class I (CI) cytosolic small heat shock (sHsps), was identified interactions...
Background It is essential to consider a practical antibody test successfully implement marker vaccines and validate vaccination efficacy against classical swine fever virus (CSFV). The should include serological assay, combined with tool for differentiating infected from vaccinated animals (DIVA). immunochromatographic strip (ICS) has been exclusively designed detecting CSFV E2 antibodies while lacking in E rns antibodies, which can be employed satisfy DIVA strategy. This study developed...
The oxidative damage initiated by reactive oxygen species (ROS) is a major contributor to the functional decline and disability that characterizes aging. anti-oxidant flavonoid, quercetin, plant polyphenol may be beneficial for retarding aging process. We examined restoring properties of quercetin on human dermal fibroblasts (HDFs). Quercetin directly reduced either intracellular or extracellular ROS levels in aged HDFs. To find aging-related target genes microarray analysis was performed...
Classical swine fever virus (CSFV) is highly contagious, and fatal to infected pigs. Vaccines against CSFV have been developed from attenuated or modified live viruses. These vaccines are effective for immunization of animals, but they associated with problems such as the accidental spreading viruses animals in field, barriers trade following vaccination. Here, we report generation transgenic Nicotiana benthamiana plants large-scale, cost-effective production E2 fusion protein use a...
Influenza epidemics frequently and unpredictably break out all over the world, seriously affect breeding industry human activity. Inactivated live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety. Subunit enjoy biosafety specificity a few weak points compared to inactivated virus or vaccines, especially in low immunogenicity. In this study, we developed new subunit vaccine platform potent, adjuvant-free, multivalent vaccination. The ectodomains of...
The ongoing coronavirus disease 2019 (COVID-19) pandemic has spurred rapid development of vaccines as part the public health response. However, general strategy used to construct recombinant trimeric severe acute respiratory syndrome 2 (SARS-CoV-2) spike (S) proteins in mammalian cells is not completely adaptive molecular farming. Therefore, we generated several constructs S for high expression Nicotiana benthamiana. Intramuscular injection N. benthamiana-expressed Sct vaccine (NSct Vac)...
Abstract Zika virus infection causes multiple clinical issues, including Guillain–Barré syndrome and neonatal malformation. Vaccination is considered as the only strategy for prevention of ZIKV-induced issues. This study developed a plant-based recombinant vaccine that transiently expressed ZIKV envelope protein (ZikaEnv:aghFc) in Nicotiana benthamiana evaluated protective immunity afforded by it immunocompetent mice. ZikaEnv:aghFc induced both humoral cellular at low dose (1–5 μg)....
Classical swine fever (CSF) is one of the most important viral diseases worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it difficult distinguish vaccinated pigs from infected pigs; this leads restrictions on import and export. Subunit based CSFV E2 glycoprotein developed using baculovirus insect cell systems, but some weaknesses remain. Here, we describe production an recombinant protein a Nicotiana benthamiana plant expression system. To do this, took...
The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A Nicotiana benthamiana tissue culture system used obtain stable production E2-glycoprotein fused porcine Fc region IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four each) vaccinated with either 100 μg/dose or 300 at 64 days pregnancy. Group 3 (two sows) injected PBS. boosted same...
Newborn piglets are susceptible to a highly contagious enteritis caused by the porcine epidemic diarrhea virus (PEDV), associated with high levels of mortality worldwide. There is pressing need for rapid, safe, and cost-effective vaccine safeguard pigs from getting infected PEDV. PEDV belongs coronavirus family characterized mutability. The primary goal provide immunity newborn through vaccination sows. Plant-based vaccines becoming more popular because they have low manufacturing costs,...
Introduction African swine fever (ASF) is a lethal and highly contagious transboundary animal disease with the potential for rapid international spread. In absence of widely available definitively proven vaccine, early detection critical ASF control. The quick user-friendly lateral flow assay (LFA) can easily be performed by following simple instructions ideal on-site use. This study describes development validation two LFAs virus (ASFV) in pig serum. Methods immunogenic antigens (p30 p72)...
A classical swine fever virus (CSFV)-modified live LOM (low-virulence strain of Miyagi) vaccine (MLV-LOM) to combat CSF has been used in places where the disease is prevalent around world, including Korea, except Jeju Island. In general, modified virus-based vaccines (MLV) are known be highly effective inducing immune responses. At same time, MLVs also have potential dangers such as a circulation field. There still need for safer and more control this study, we applied new based on...
Coronavirus disease 2019 (COVID-19) is a novel infectious respiratory caused by SARS-CoV-2. We evaluated the efficacy of plant-based human recombinant angiotensin-converting enzyme 2 (hrACE2) and hrACE2-foldon (hrACE2-Fd) protein against COVID-19. In addition, we analyzed antiviral activity hrACE2 hrACE2-Fd SARS-CoV-2 using real-time reverse-transcription PCR plaque assays. The therapeutic was detected Golden Syrian hamster model infected with Both inhibited 50% at concentrations below...
Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen mice | Youngmin Park, Hyangju Kang, Kyungmin Min, Nam Hyung Kim, Minhee In-Ohk Ouh, Ha-Hyun Jae-Young Song, Dong-Kun Yang, Eun-Ju Sohn, Sangmin Lee Agricultural Journals
Abstract Background The objective of this field trial was to evaluate the efficacy a new plant‐based porcine circovirus type 2a (PCV2a) vaccine. This vaccine recombinant capsid subunit PCV2a based on Nicotiana benthamiana expression system. Methods Three farms were selected for study their history subclinical PCV2 infection. A total 40 18‐day‐old pigs randomly allocated either vaccinated or unvaccinated groups (20 per group; 10 = male and female). Pigs received 2.0‐mL dose intramuscularly at...
Various vaccines have been developed to fight severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for disease 2019 pandemic. However, new variants of SARS-CoV-2 undermine effort SARS-CoV-2. Here, we produced S proteins harboring receptor-binding domain (RBD) Omicron variant in plants. Plant-produced together with adjuvant CIA09A triggered strong immune responses mice. Antibodies serum inhibited interaction recombinant human angiotensin-converting enzyme RBD...
<title>Abstract</title> Utilizing plants for the manufacturing of therapeutic drugs human and animal disease treatment faces technical regulatory challenges because disparities in N-glycosylation pathway between animals plants. The key challenge involves differences post-translational modification machinery pathway. We used multiplex CRISPR/Cas9 genome editing to target five α-1,3-fucosyltransferase two β-1,2-xylosyltransferase genes modify <italic>Nicotiana benthamiana</italic>. obtained...