A5055679423- Virus-based gene therapy research
- Transgenic Plants and Applications
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Plant Virus Research Studies
- Toxin Mechanisms and Immunotoxins
- Cancer Genomics and Diagnostics
- Plant tissue culture and regeneration
- Viral gastroenteritis research and epidemiology
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- SARS-CoV-2 and COVID-19 Research
- Plant Stress Responses and Tolerance
- Influenza Virus Research Studies
- Microbial Inactivation Methods
- Cancer, Stress, Anesthesia, and Immune Response
- Legume Nitrogen Fixing Symbiosis
- Axon Guidance and Neuronal Signaling
- Monoclonal and Polyclonal Antibodies Research
- Plant Reproductive Biology
- Viral-associated cancers and disorders
- Cancer Cells and Metastasis
- Cellular transport and secretion
University of Oxford
2024-2025
Pohang University of Science and Technology
2021-2025
Sun Yat-sen Memorial Hospital
2023-2024
Sun Yat-sen University
2023-2024
Chinese University of Hong Kong
2024
Tobacco Research Institute
2024
Chinese Academy of Agricultural Sciences
2024
Beijing Academy of Science and Technology
2024
Shandong Agricultural University
2015-2018
Guangzhou Experimental Station
2018
Plant vacuoles are versatile organelles critical for plant growth and responses to environment. Vacuolar proteins transported from the endoplasmic reticulum via multiple routes in plants. Two classic bear great similarity other phyla with major regulators known, such as COPII Rab5 GTPases. By contrast, vacuolar trafficking mediated by adaptor protein-3 (AP-3) or that independent of Golgi has few recognized cargos none regulators. In search novel using a fluorescence-based forward genetic...
Stomatal movement, critical for photobiosynthesis, respiration, and stress responses, is regulated by many factors, among which abscisic acid (ABA) critical. Early events of ABA signaling involve Ca2+ influx an increase cytoplasmic calcium ([Ca2+]cyt). Positive regulators this process have been extensively studied, whereas negative are obscure. ABA-induced stomatal closure involves K+ flux vacuolar convolution. How these processes connected with not fully understood. We report that pat10-1,...
ABSTRACT Agroinfiltration of Nicotiana benthamiana is routinely used for transient gene expression in plant sciences and molecular pharming. Here, we depleted transcripts 21 different immunity-related genes through virus-induced silencing (VIGS) to identify that hamper juvenile plants. These experiments uncovered ethylene insensitive- 2 ( EIN2 ), receptor-like kinase CERK1 , transcription factor regulator nonexpressor pathogenesis-related 1 NPR1 ) isochorismate synthase ICS increases GFP by...
Background/Objectives: Since December 2019, the COVID-19 pandemic, driven by SARS-CoV-2, has caused ~690 million infections globally, manifesting with mild to severe symptoms, including pneumonia. After reduced activity, seasonal influenza re-emerged in winter 2022, creating a “twindemic” SARS-CoV-2. Co-infections have been associated higher risks, such as increased ventilator use and mortality, emphasizing need for dual-target vaccines. This study investigates plant-based vaccines produced...
The infiltration of Nicotiana benthamiana with Agrobacterium tumefaciens (agroinfiltration) has become a routine expression platform for plant science and molecular pharming, yet this remains to be further optimized. We recently showed that N. silenced the cold shock protein (CSP) receptor (CORE) enables 8-fold more GFP production in older, 6–8-week-old plants, which are normally not used because low transient efficiencies (Dodds et al., 2023). Here, we investigated whether can also increase...
Oxidation in extracts from agroinfiltrated leaves leads to browning and protein precipitation. Here we show that silencing of polyphenol oxidase (PPO) Nicotiana benthamiana suppresses enzymatic avoids crosslinking endogenous proteins such as RuBisCo. PPO does not impact plant growth or development may also enhance transient gene expression. These results depletion activity could be highly beneficial for science applications molecular pharming.
Rhos of plants (ROPs) play a key role in plant cell morphogenesis, especially tip-growing pollen tubes and root hairs, by regulating an array intracellular activities such as dynamic polymerization actin microfilaments. ROPs are regulated guanine nucleotide exchange factors (RopGEFs), GTPase activating proteins (RopGAPs), dissociation inhibitors (RhoGDIs). RopGEFs RopGAPs evolutionarily conserved function ROP signaling. By contrast, although RhoGDIs regulate the membrane extraction...
Abstract We report here that Arabidopsis PROTEIN S-ACYL TRANSFERASE14 (PAT14), through its palmitate transferase activity, acts at the vacuolar trafficking route to repress salicylic acid (SA) signaling, thus mediating age-dependent but not carbon starvation-induced leaf senescence. Functional loss of PAT14 resulted in precocious senescence and transcriptomic analysis revealed was dependent on acid. Overexpressing suppressed expression SA responsive genes. Introducing deficient mutants,...
Abstract Plants offer a powerful platform for recombinant protein production but degradation of proteins by endogenous proteases is causing severe yield losses. Here, we introduce triple knockout lines SBT5.2, the major active subtilases in apoplast agroinfiltrated Nicotiana benthamiana . HIV-neutralising IgG antibody 2F5 no longer cleaved sbt5.2 mutants and these accumulate 3-fold more upon transient expression grow normally. Remarkably, however, does not exposed to SBT5.2 when transiently...
Agroinfiltration of Nicotiana benthamiana has emerged as a main protein expression platform in plant science and molecular pharming but yields are often hampered by endogenous proteases degrading recombinant proteins. Many IgG antibodies, for instance, degraded when expressed N. (Niemer et al., 2014). HIV-neutralizing antibody 2F5 is cleaved the H3 loop variable region heavy chain (HC) incubated apoplastic fluids (AF) benthamiana, this processing was blocked with Ser protease inhibitor PMSF...
Influenza epidemics frequently and unpredictably break out all over the world, seriously affect breeding industry human activity. Inactivated live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety. Subunit enjoy biosafety specificity a few weak points compared to inactivated virus or vaccines, especially in low immunogenicity. In this study, we developed new subunit vaccine platform potent, adjuvant-free, multivalent vaccination. The ectodomains of...
The ongoing coronavirus disease 2019 (COVID-19) pandemic has spurred rapid development of vaccines as part the public health response. However, general strategy used to construct recombinant trimeric severe acute respiratory syndrome 2 (SARS-CoV-2) spike (S) proteins in mammalian cells is not completely adaptive molecular farming. Therefore, we generated several constructs S for high expression Nicotiana benthamiana. Intramuscular injection N. benthamiana-expressed Sct vaccine (NSct Vac)...
Plants have long been considered a cost-effective platform for recombinant production. A recently recognized additional advantage includes the low risk of contamination human pathogens, such as viruses and bacterial endotoxins. Indeed, great advance has made in developing plants “factory” to produce proteins use biopharmaceutical purposes. However, there is still need develop new tools protein production plants. In this study, we provide data showing that B1 domain Streptococcal G (GB1) can...
Plants have recently received much attention as a means of producing recombinant proteins because they are easy to grow at low cost and large scale. Although many plant protein expression systems been developed, there remains need for improved that deliver high yields proteins. Transcription the gene is key step in increasing yield However, revealed strong promoters, terminators, transcription factors identified do not necessarily lead level production Thus, this study, robust system was...
<div>Abstract<p>Chronic inflammation induced by persistent microbial infection plays an essential role in tumor progression. Although it is well documented that Epstein–Barr virus (EBV) closely associated with nasopharyngeal carcinoma (NPC), how EBV-induced promotes NPC progression remains largely unknown. Here, we report infiltration of tumor-associated macrophages (TAM) and expression CCL18, the cytokine preferentially secreted TAM, correlate serum EBV titers two cohorts...
<p>supplemental text includes supplemental figure legend, experimental procedures and reference.</p>
<p>Supplemental Table S1. Baseline characteristics of NPC patients training and validation cohort. Supplemental S2. Predicted cutoff for the number CD68 or CCL18 cells in nasopharyngeal carcinoma cohort analysed by X-tile. S3. Correlation Expressing TAM Counts with Clinicopathological Status (321 Cases) (259 cases) Patients carcinoma.</p>
<p>Figure S3 related to Figure 3. EBV-replicating NPC cells recruit monocytes and activate TAMs via VEGF GM-CSF respectively.</p>
<p>Figure S5 related to Figure 5. TAMs induce EMT and enhance the invasiveness of NPC cells through CCL18</p>