A5009550935- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Single-cell and spatial transcriptomics
- Extracellular vesicles in disease
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Reproductive tract infections research
- Immune responses and vaccinations
- HIV/AIDS Research and Interventions
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Reproductive System and Pregnancy
- Long-Term Effects of COVID-19
- HIV-related health complications and treatments
- Spatial Cognition and Navigation
- Stress Responses and Cortisol
- Inflammasome and immune disorders
- COVID-19 epidemiological studies
- Virology and Viral Diseases
- Immune cells in cancer
- Cell death mechanisms and regulation
Emory University
2016-2025
Emory National Primate Research Center
2014-2024
Emory and Henry College
2019-2024
Columbia University
2021-2023
ICAP Global Health
2021-2023
Ministry of Public Health
2023
Woodruff Health Sciences Center
2014
University of California, Berkeley
1990-1993
Ames Research Center
1990-1992
While CD4+ T cell depletion is key to disease progression in people living with HIV and SIV-infected macaques, the mechanisms underlying this remain incompletely understood, most death involving uninfected cells. In contrast, SIV infection of "natural" hosts such as sooty mangabeys does not cause AIDS despite high-level viremia. Here, we report that CARD8 inflammasome activated immediately after entry by viral protease encapsulated incoming virions. Sensing activity leads rapid pyroptosis...
The rhesus macaque (Macaca mulatta) is a key species for advancing biomedical research. Like all draft mammalian genomes, the assembly (rheMac2) has gaps, sequencing errors and misassemblies that have prevented automated annotation pipelines from functioning correctly. Another assembly, CR_1.0, also available but substantially more fragmented than rheMac2 with smaller contigs scaffolds. Annotations these two assemblies are limited in completeness accuracy. High quality files required wide...
Significance Simian immunodeficiency virus (SIV)-specific follicular CD8 T cells represent a unique subset of antiviral that rapidly expand during pathogenic SIV infection, localize within B-cell follicles, and contribute to control chronic replication. The potential for these infiltrate sites ongoing viral replication persistence the ability induce by vaccination provide tremendous opportunity develop optimize therapeutic strategies target reduce HIV reservoirs in lymphoid tissues.
Belatacept is a B7-specific fusion protein used to prevent allograft rejection by blocking T cell costimulation. Generally efficacious, it fails acute in sizable minority of patients. In experimental models, memory cells mediate costimulation blockade–resistant (CoBRR), but this remains undefined humans. To explore relationships between individual patients' immune phenotypes and CoBRR, we studied patients receiving belatacept or conventional calcineurin inhibitor–based immunosuppression. We...
Whole-genome sequencing and comparative genomic analysis of immune-related genes Cercocebus atys Macaca mulatta identify candidate genes, such as ICAM2 TLR4, that may explain the AIDS resistance C. atys. Sooty mangabeys are a primate model for non-pathogenic simian immunodeficiency virus (SIV) infection. Guido Silvestri colleagues sequenced genome captive sooty mangabey from Yerkes National Primate Research Center in Atlanta, Georgia. They compared this with sequences other primates suggest...
The immunopathological mechanisms driving the development of severe COVID-19 remain poorly defined. Here, we utilize a rhesus macaque model acute SARS-CoV-2 infection to delineate perturbations in innate immune system. initiates rapid infiltration plasmacytoid dendritic cells into lower airway, commensurate with IFNA production, natural killer cell activation, and significant increase blood CD14
HIV infection persists during antiretroviral therapy (ART) due to a reservoir of latently infected cells that harbor replication-competent virus and evade immunity. Previous ex vivo studies suggested CD8
The minimally invasive nature of endoscopic surgery allows operations to be performed through small incisions, producing significantly less damage good tissue than in conventional open surgery. Patient recovery times are thus greatly reduced. This major advantage has driven a dramatic growth techniques wide range surgical applications. Unfortunately, the surgeon's abilities severely hampered by limitations current technology. In many ways is similar teleoperation remote manipulator. Although...
Therapeutic strategies that augment antiviral immunity and reduce the viral reservoir are critical to achieving durable remission of HIV. The coinhibitory receptor programmed death-1 (PD-1) regulates CD8+ T cell dysfunction during chronic HIV SIV infections. We previously demonstrated in vivo blockade PD-1 infection improves function cells B cells. Here, we tested immunological virological effects combined with antiretroviral therapy (ART) rhesus macaques. Administration anti-PD-1 antibody...
B cells play a critical role in the immune response by producing antibodies, which display remarkable diversity. Here we describe bioinformatic pipeline, BALDR (BCR Assignment of Lineage using De novo Reconstruction) that accurately reconstructs paired heavy and light chain immunoglobulin gene sequences from Illumina single-cell RNA-seq data. was accurate for clonotype identification human rhesus macaque influenza vaccine simian immunodeficiency virus induced vaccine-induced plasmablasts...
Type I interferons (IFN-I) are critical mediators of innate control viral infections but also drive the recruitment inflammatory cells to sites infection, a key feature severe coronavirus disease 2019. Here, IFN-I signaling was modulated in rhesus macaques (RMs) before and during acute SARS-CoV-2 (severe respiratory syndrome 2) infection using mutated IFN-α2 (IFN-modulator; IFNmod), which has previously been shown reduce binding endogenous IFN-I. IFNmod treatment uninfected RMs observed...
Transcriptomic profiling of primate T cells during acute graft-versus-host disease reveals signaling pathways that when inhibited, ameliorate disease.
Chronic malaria is a major public health problem and significant challenge for disease eradication efforts. Despite its importance, the biological factors underpinning chronic are not fully understood. Recent studies have shown that host metabolic state can influence pathogenesis transmission, but role in chronicity known. Here, with goal of identifying distinct modifications metabolite profiles acute versus malaria, metabolomics was performed on plasma from Plasmodium-infected humans...
ABSTRACT Zika virus (ZIKV) is a mosquito-borne flavivirus that caused an epidemic in the Americas 2016 and linked to severe neonatal birth defects, including microcephaly spontaneous abortion. To better understand host response ZIKV infection, we adapted 10× Genomics Chromium single-cell RNA sequencing (scRNA-seq) assay simultaneously capture viral mRNA. Using this assay, profiled antiviral landscape population of human monocyte-derived dendritic cells infected with at level. The bystander...
The genome of the white-throated sparrow (Zonotrichia albicollis) contains an inversion polymorphism on chromosome 2 that is linked to predictable variation in a suite phenotypic traits including plumage color, aggression and parental behavior. Differences gene expression between two color morphs, which represent common genotypes (ZAL2/ZAL2 ZAL2/ZAL2(m) ), may therefore advance our understanding molecular underpinnings these phenotypes. To identify genes are differentially expressed morphs...