Abstract We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows discovery of greater than 95% all expected heterozygous singe base variants, requires as little 3 Gbp raw sequence data and constitutes an effective tool identifying rare coding alleles in large scale genomic studies.

Generation of long (>5 Kb) DNA sequencing reads provides an approach for interrogation complex regions in the human genome. Currently, large-insert whole genome (WGS) technologies from Pacific Biosciences (PacBio) enable analysis chromosomal structural variations (SVs), but cost to achieve required sequence coverage across entire is high.We developed a method (termed PacBio-LITS) that combines oligonucleotide-based target-capture enrichment with PacBio library preparation facilitate SV...

Purpose This paper examines the relationship of entrepreneurial orientation (EO), new product development (NPD), legitimacy (political and market) firm performance (FP). The authors investigate how when EO improves FP in high-tech small medium-size enterprises (SMEs). Design/methodology/approach formulates 5 hypotheses from literature review theoretical deduction. are tested using ordinary least squares (OLS) regression with data collected 219 randomly selected SMEs operating industries...

A new robust neuro-fuzzy controller for autonomous and intelligent robot manipulators in dynamic partially known environments containing moving obstacles is presented. The navigation based on a fuzzy technique the idea of artificial potential fields (APFs) using analytic harmonic functions. Unlike technique, development APFs computationally intensive. efficient processing scheme to reasoning about obstacle avoidance APF described, namely, motion planning. An integration modified Elman neural...

The contents of working memory (WM) can affect the subsequent visual search performance, resulting in either beneficial or cost effects, when target is included spatially dissociated from memorized contents, respectively. right dorsolateral prefrontal cortex (rDLPFC) and posterior parietal (rPPC) have been suggested to be associated with congruence/incongruence effects content target. Thus, present study, we investigated role DLPFC PPC controlling interaction between WM attention during a...

Schizophrenia (SZ) is a complex disease. Single nucleotide polymorphism (SNP), brain activity measured by functional magnetic resonance imaging (fMRI) and DNA methylation are all important biomarkers that can be used for the study of SZ. To our knowledge, there has been little effort to combine these three datasets together. In this study, we propose group sparse joint nonnegative matrix factorization (GSJNMF) model integrate SNP, fMRI, identification multi-dimensional modules associated...

The ability to focus on task-relevant information, while suppressing distraction, is critical for human cognition and behavior. Using a delayed-match-to-sample (DMS) task, we investigated the effects of emotional face distractors (positive, negative, neutral faces) early late phases visual short-term memory (VSTM) maintenance intervals, using low high VSTM loads. Behavioral results showed decreased accuracy delayed reaction times (RTs) vs. load. Event-related potentials (ERPs) enhanced...

Purpose To develop and internally validate a nomogram combining radiomics signature of primary tumor fibroglandular tissue (FGT) based on pharmacokinetic dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) clinical factors for preoperative prediction sentinel lymph node (SLN) status in breast cancer patients. Methods This study retrospectively enrolled 186 patients who underwent pretreatment DCE-MRI with positive ( n = 93) negative SLN. Logistic regression models signatures FGT...

Abstract Background Focal cortical dysplasia type 2 (FCD2) is a malformation of development that constitutes common cause pediatric focal epilepsy. Germline or somatic variants in the mammalian target rapamycin (mTOR) signaling pathway genes are pathogenesis FCD2. Objective In this study, whole‐exome deep sequencing was performed on dysplastic cortex from epilepsy children to explore genetic characteristics Methods Resected core lesions FCD2 were confirmed by pathology, and peripheral blood...

Corporate social responsibility (CSR) strategy hinges largely on the CEO characteristics in context of an emerging market. Based a sample 16,144 firm-year observations obtained from 1,370 unique Chinese-listed firms, which whether voluntarily issue CSR reports over period 2008–2019, this paper empirically examined impact likelihood issuing reports. We find that age, MBA education, international experience and political ideology consciousness are positively associated with possibility...

Abstract Multiplex‐fluorescence in situ hybridization (M‐FISH) is a chromosome imaging technique which can be used to detect chromosomal abnormalities such as translocations, deletions, duplications, and inversions. Chromosome classification from M‐FISH data key step implement the technique. In classified image, each pixel labeled with class index drawn pseudo‐color so that geneticists easily conduct diagnosis, for example, identifying translocations by examining color changes between...

Background: Congenital contractural arachnodactyly (CCA) is an autosomal dominant connective tissue disorder with clinical features of arthrogryposis, arachnodactyly, crumpled ears, scoliosis, and muscular hypoplasia. The heterozygous pathogenic variants in FBN2 have been shown to cause CCA. Fibrillin-2 related the elasticity has demonstrated play important role constitution extracellular microfibrils elastic fibers, providing strength flexibility that sustains body’s joints organs. Methods:...

Multi-cue integration has proved successful at increasing the robustness of tracking algorithms and overcoming failure cases individual cue. But considering dynamic appearance objects or clutter background, based on constant weights may weaken performance this scheme. In paper, we propose a update mechanism for multiple cues with detection responses as supervision. We integrate observation hypotheses compared association results adjust according to approximation degree. The is adapted...

Remote sensing images often suffer from stripe noise, which greatly degrades the image quality. Destriping of remote is to recover a good containing noise. Since stripes in have directional characteristic (horizontal or vertical), unidirectional total variation has been used consider information and preserve edges. The contaminated by heavy noise always large width pixels low correlations with true pixels. On this occasion, destriping process can be viewed as inpainting wide domains. In many...

To study the feasibility of use radiomic features extracted from axillary lymph nodes for diagnosis their metastatic status in patients with breast cancer.A total 176 cancer, consisting 87 (ALNM) and 89 negative proven by surgery, were retrospectively reviewed database our cancer center. For each selected node, 106 based on preoperative pharmacokinetic modeling dynamic contrast enhanced magnetic resonance imaging (PK-DCE-MRI) 5 conventional image obtained. The least absolute shrinkage...

In task-switching paradigms, reaction times (RTs) switch cost (SC) and the neural correlates underlying SC are affected by different preparation intervals. However, little is known about effect of interval on repetition processes in task-switching. To examine this we utilized a cued paradigm with long sequences repeated trials. Response-stimulus intervals (RSI) cue-stimulus (CSI) were manipulated short conditions. Electroencephalography (EEG) behavioral data recorded. We found that...

Abstract Background In this study, we aimed to investigate the role of miR-26a and miR-26b in management AF. Methods Real-time PCR was carried out determine plasma microRNA expression AF patients pre- post-radiofrequency ablation. The correlation between SELP miR-26a/miR-26b also studied using luciferase assays establish a miR-26a/miR-26b/SELP signaling pathway. Results relative reached its peak pre-ablation ( +) patients, while ablation treatment reduced patients. Similarly, pigs showed...

Recently, analog visual transmission has attracted considerable attention owing to its graceful performance degradation for various wireless channels. In this study, we propose a novel communications system, named DVCast, in which an image denoising algorithm is used fully utilize spatial correlation; moreover, the variable block size Discrete Cosine Transform (DCT) preserve more correlation information image. Obviously, there tradeoff between system and computing complexity. Therefore,...

To investigate the changes of three-dimensional apparent diffusion coefficient (3D-ADC) bilateral parotid glands during radiotherapy for head and neck squamous cell carcinoma (HNSCC) patients explore correlations with radiation dose, volume reduction gland salivary secretary function. 60 HNSCC were retrospectively collected in Sichuan cancer hospital. The all received diffusion-weighted imaging (DWI) scan at pre-radiation, 15th radiation, 25th completion radiation. Dynamic 3D-ADC measured...

With the advent of era artificial intelligence, "scenario" frequently appears in new product development and has gradually become an effective tool for analyzing user needs. However, reasons this phenomenon have not been explored depth. New is a creative activity that requires designers to imagine how people will live near future. So, we speculated familiar scenario matches designers' background (including knowledge, expertise, experience) can spark their entrepreneurial imaginativeness by...