A5085399576- Blood Coagulation and Thrombosis Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Atrial Fibrillation Management and Outcomes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Pharmacogenetics and Drug Metabolism
- Protease and Inhibitor Mechanisms
- Vitamin K Research Studies
- Renin-Angiotensin System Studies
- Hemophilia Treatment and Research
- Central Venous Catheters and Hemodialysis
- Acute Myocardial Infarction Research
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Blood properties and coagulation
- Eicosanoids and Hypertension Pharmacology
- Retinal Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Immunotherapy and Immune Responses
- S100 Proteins and Annexins
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Hormonal Regulation and Hypertension
- Liver Disease Diagnosis and Treatment
- Acute Ischemic Stroke Management
Universidad de Navarra
2012-2025
Centro de Investigación en Red en Enfermedades Cardiovasculares
2018-2022
Navarre Institute of Health Research
2022
Complejo Hospitalario de Navarra
2022
Instituto de Salud Carlos III
2018-2022
Centro de Investigación Biomédica en Red
2018
Instituto de Investigación Biomédica de Salamanca
2016
Clinica Universidad de Navarra
2002-2016
Universidad de Murcia
2014
Centro Regional de Hemodonación
2014
Different isoforms of VEGF-A (mainly VEGF₁₂₁, VEGF₁₆₅ and VEGF189) have been shown to display particular angiogenic properties in the generation a functional tumor vasculature. Recently, novel class isoforms, designated as VEGF(xxx)b, generated through alternative splicing, described. Previous studies suggested that these may inhibit angiogenesis. In present work we produced recombinant VEGF₁₂₁/₁₆₅b proteins yeast Pichia pastoris constructed vectors overexpress assess their...
Down-regulation of CD4+CD25+ regulatory T (Treg) cell function might be beneficial to enhance the immunogenicity viral and tumor vaccines or induce breakdown immunotolerance. Although mechanism suppression used by Treg cells remains controversial, it has been postulated that TGF-beta1 mediates their immunosuppressive activity. In this study, we show P17, a short synthetic peptide inhibits TGF-beta2 developed in our laboratory, is able inhibit activity vitro vivo. studies demonstrate P17...
Oxidative stress plays a critical role in the pathogenesis of hypertension. The NADPH oxidase constitutes major source superoxide anion phagocytic cells, and its activation is associated with matrix metalloproteinase (MMP)-9 secretion by these cells. We investigated effects angiotensin II type 1 receptor antagonist losartan metabolites (EXP3174 EXP3179) on activity MMP-9 human EXP3179, but not EXP3174, dose-dependently inhibited (P<0.05) phorbol myristate acetate insulin-stimulated activity....
Efficient metastasis requires survival and adaptation of tumor cells to stringent conditions imposed by the extracellular milieu. Identification critical signaling pathways in might unveil novel targets relevant disease progression.To investigate contribution activated protein C (APC) its receptor (endothelial [EPCR]) animal models lung cancer patients with adenocarcinoma.Signaling pathway triggered APC/EPCR relevance apoptosis was studied vitro. Functional significance assessed silencing...
The Endothelial Protein C receptor (EPCR) is essential for the anticoagulant and cytoprotective functions of (PC) system. Selected variants malaria parasite protein, Plasmodium falciparum Erythrocyte Membrane 1 (PfEMP1) associated with severe malaria, including cerebral specifically target EPCR on vascular endothelial cells. Here, we examine cellular response to PfEMP1 engagement elucidate its role in pathogenesis. Binding CIDRα1.1 domain obstructed activated PC (APC) binding induced a loss...
The outcome of venous thromboembolism in patients with cancer is worse than without cancer, a higher risk recurrences, bleeding and death. However, these risks appear to vary depending on the type. While some tumours recurrences outweighs bleeding, others major (MB) during anticoagulation markedly exceeds recurrent event. Balancing could be helpful tailor management cancer-associated thrombosis (CAT) improve outcomes. In this article, published data from recent randomized clinical trials as...
Summary Much of the variability in sensitivity to warfarin anticoagulated patients is associated with c. − 1639G > A polymorphism vitamin K‐epoxide reductase ( VKORC1 ) gene. However, its association acenocoumarol dose under anticoagulant therapy has not been studied. The genotype was determined 113 on stable anticoagulation requiring low n = 42), medium 42) or high 21) doses. To evaluate between requirements and variant, multivariate logistic regression models were fitted, adjusting for...
Atrial fibrillation (AF) is a major risk factor for cardio-embolic stroke. Anticoagulant drugs are effective in preventing AF-related However, the high frequency of anticoagulant-associated bleeding concern. This study sought to identify new targets develop safer antithrombotic therapies. Here, microarray analysis peripheral blood cells eight patients with AF and stroke subjects without brought light stroke-related gene expression pattern. HSPA1B, which encodes heat-shock protein 70 kDa...
Summary Haemostasis plays an integral role in arterial thrombotic disease. However, establishing which of the factors are risk has proven surprisingly difficult. Because its technical simplicity and digital nature, study haemostatic polymorphisms as grown popularity. Once established a factor, genetic polymorphism potential to aid selective prophylaxis therapy Numerous reports have now been published on coagulation fibrinolytic factors, inhibitory proteins, platelet membrane glycoprotein...
Uniquely amongst vitamin K‐dependent coagulation proteins, protein C interacts via its Gla domain both with a receptor, the endothelial cell receptor (EPCR), and phospholipids. We have studied naturally occurring recombinant variants for soluble (s)EPCR binding, surface activation to activated (APC) by thrombin–thrombomodulin complex, phospholipid dependent factor Va (FVa) inactivation APC, establish if these functions are concordant. Wild‐type binding sEPCR was characterized plasmon...
Summary Many cancer patients are at high risk of venous thromboembolism (VTE) during hospitalisation; nevertheless, thromboprophylaxis is frequently underused. Electronic alerts (e-alerts) have been associated with improvement in use and a reduction the incidence VTE, both hospitalisation after discharge, particularly medical setting. However, there no data regarding benefit this tool patients. Our aim was to evaluate impact computer-alert system for VTE prevention cancer, those admitted...
Activated protein C/endothelial C receptor (APC/EPCR) axis is physiologically involved in anticoagulant and cytoprotective activities endothelial cells. Emerging evidence indicates that EPCR also plays a role breast stemness human tumorigenesis. Yet, its contribution to cancer progression metastasis has not been elucidated. Transcriptomic status of was examined cohort 286 patients. Cell growth kinetics evaluated control SPARC/osteonectin, Cwcv, kazal-like domains proteoglycan...
Summary Plasminogen activator inhibitor-1 (PAI-1) increases in endotoxemia thus possibly cooperating altering the hemostatic balance a prothrombotic direction. The effect of inhibition PAI-1 with monoclonal antibody MA-33B8 was studied systemically and kidneys lapine model endotoxin-induced disseminated intravascular coagulation (DIC). increase plasmatic PAI activity control group (n = 9) inhibited treated rabbits 5). Control showed renal fibrin deposits, whereas only one did so. These...
Summary The endothelial protein C receptor (EPCR) plays an anticoagulant role by improving activation. Although low levels of activated (APC) constitute a thrombosis risk factor, the relationship between modulating EPCR function and has not been addressed so far. Monoclonal antibodies (mAb) against murine were raised, their ability to block C/APC binding was tested. ferric chloride carotid artery injury model in mice chosen test effect anti-EPCR mAb on thrombus formation. time total...