Abstract Background Emerging evidence indicates that circular RNAs (circRNAs) and m 6 A RNA methylation participate in the pathogenesis metastasis of multiple malignancies including hepatocellular carcinoma (HCC). However, it remains undocumented how circRNAs form a feedback loop with modification contributing to HCC. Methods novel hsa_circ_0017114 (circGPR137B) was identified from three pairs primary HCC adjacent normal tissues by circRNA expression profiling. The association circGPR137B...

Summary H 2 O is a major reactive oxygen species produced by mitochondria that implicated to be important in aging and pathogenesis of diseases such as diabetes; however, the cellular physiological roles mitochondrial remain poorly understood. Peroxiredoxin 3 (Prdx3/Prx3) thioredoxin peroxidase localized mitochondria. To understand age‐associated diseases, we generated transgenic mice overexpressing Prdx3 (Tg(PRDX3) mice). Tg(PRDX3) overexpress broad range tissues, overexpression occurs...

Increased lipid peroxidation is shown to be an early event of Alzheimer's disease (AD). However, it not clear whether and how increased might lead amyloidogenesis, a hallmark AD. Glutathione peroxidase 4 (Gpx4) essential antioxidant defense enzyme that protects organism against peroxidation. Gpx4+/- mice show in brain, as evidenced by their elevated levels 4-hydroxy-2-nonenal. To understand the role we studied secretase activities function age. Both young (6 months) middle-aged (17-20 had...

Abstract Polyunsaturated fatty acids (PUFA) in membrane lipids are prone to attack by reactive oxygen species (ROS), and the resulting lipid peroxidation can cause injury death of cells. Glutathione peroxidase 4 (Gpx4) is an antioxidant defense enzyme that directly detoxify hydroperoxides generated ROS. Overexpression Gpx4 has been shown be protective against oxidative damage several cell lines. We examined this study stress response neurons with increased expression Gpx4, because especially...

The sudden outbreak of COVID-19 has led to more than seven thousand deaths. Unfortunately, there are no specific drugs available cure this disease. Type 2 taste receptors (TAS2Rs) may play an important role in host defense mechanisms. Based on the idea host-directed therapy (HDT), we performed a negative co-expression analysis using big data 60 000 Affymetrix expression arrays and 5000 TCGA sets determine functions TAS2R10, which can be activated by numerous bitter substances. Excitingly,...

Disease name normalization is an important task in the medical domain. It classifies disease names written various formats into standardized names, serving as a fundamental component smart healthcare systems for disease-related functions. Nevertheless, most significant obstacle to existing severe shortage of training data. Consequently, we present novel data augmentation approach that includes series techniques and some supporting modules help mitigate problem. Through extensive...

BACKGROUND Diabetic nephropathy (DN) is a disease characterized by oxidative stress and apoptosis of renal tubular epithelial cells driven hyperglycemia. Apigenin flavonoid compound that possesses potent anti‑apoptotic properties. The present study aimed to explore the protective effects underlying mechanisms apigenin on exposed MATERIAL AND METHODS Human cell HK-2 were incubated D-glucose establish in vitro DN model. viability, lactate dehydrogenase (LDH) release, evaluated. qRT-PCR was...

Small conductance Ca(2+)-activated K(+) (SK) channels regulate membrane properties of rostral ventrolateral medulla (RVLM) projecting hypothalamic paraventricular nucleus (PVN) neurons and inhibition SK increases in vitro excitability. Here, we determined vivo the role PVN regulating sympathetic nerve activity (SNA) mean arterial pressure (MAP). In anesthetized rats, bilateral microinjection channel blocker with peptide apamin (0, 0.125, 1.25, 3.75, 12.5, 25 pmol) increased splanchnic SNA...

Renal tubular damage is critical pathological feathers of diabetic nephropathy (DN). This study aimed to explore the protective activity and related mechanisms crocin in renal epithelial cell injury induced by high glucose.Renal HK-2 cells were cultured with D-glucose establish an vitro DN model. Cell viability was evaluated CCK-8 assay. Apoptosis detected Annexin V-FITC kit. Oxidative stress colorimetry. RT-qPCR carried out determine mRNA expressions NF-E2-related factor 2 (Nrf2) its...

This study, a subgroup analysis of the data from Organization Program DiabEtes INsulIN ManaGement aimed to compare efficacy and safety profiles acarbose metformin used in combination with premixed insulin.This included 80 192 patients taking only 1 oral antidiabetic drug, classified into (treated + insulin) groups insulin), respectively. The were analyzed for within- between-group differences. clinical trial registry number was NCT01338376.The percentage who achieved target hemoglobin A1c...

Leptin acts to influence bone metabolism through indirect hypothalamic relay and direct peripheral pathways. enhances the differentiation of mesenchymal stem cells osteoblast rather than adipocyte lineage, but in vivo impacts leptin on ovariectomy (OVX)-induced marrow adiposity are poorly understood. In this work, we aimed address question.Forty-five female New Zealand rabbits were divided into sham + vehicle, OVX for 5 months. Magnetic resonance spectroscopy dual-energy x-ray absorptiometry...

Sclerostin knock-out mice or sclerostin antibody (Scl-Ab) treated wild-type displayed decreased marrow adiposity. But the effects of Scl-Ab on estrogen deficiency-induced fat expansion remain elusive. In this work, 45 female New Zealand rabbits were equally divided into sham-operation, ovariectomy controls, and with for 5 months. MR spectroscopy was performed to longitudinally assess fraction at baseline conditions, 2.5 months post-operatively, respectively. We evaluated bone mineral density...

High glucose (HG) induces vascular injury in diabetes. Hydroxysafflor yellow A (HSYA) has been used to ameliorate ischemic cardiovascular diseases China for many years. In the present study, we assessed whether HSYA a potential protective role HG-induced human umbilical vein endothelial cell (HUVEC) injury. Cell viability was determined with an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. apoptosis detected by fluorescein...

The aim of the present study was to investigate underlying molecular mechanisms osteoporosis and identify novel candidate genes involved in this disease. gene expression profile GSE35958 downloaded from Gene Expression Omnibus, including five samples human mesenchymal stem cells patients with four control samples. Differentially expressed (DEGs) were initially identified following an analysis using Student's t‑test. Subsequently, a protein‑protein interaction (PPI) network significant...