A5101601251- Antioxidant Activity and Oxidative Stress
- Free Radicals and Antioxidants
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Mitochondrial Function and Pathology
- Biotechnology and Related Fields
- Genetics, Bioinformatics, and Biomedical Research
- Genetic Neurodegenerative Diseases
- Vitamin C and Antioxidants Research
- Coenzyme Q10 studies and effects
- Science, Research, and Medicine
- Mast cells and histamine
- Nutrition, Genetics, and Disease
- Redox biology and oxidative stress
- Peripheral Neuropathies and Disorders
- Nitric Oxide and Endothelin Effects
- Fatty Acid Research and Health
- Metabolomics and Mass Spectrometry Studies
- Asthma and respiratory diseases
- Glutathione Transferases and Polymorphisms
- Biomedical Ethics and Regulation
- CRISPR and Genetic Engineering
- Advanced Glycation End Products research
- Botulinum Toxin and Related Neurological Disorders
- Urticaria and Related Conditions
St Vincent's Hospital
2015-2025
The University of Melbourne
2015-2025
St. Vincent's Birmingham
2012-2022
Monash University
2014-2022
Florey Institute of Neuroscience and Mental Health
2022
Murdoch Children's Research Institute
2022
St. Vincent's Hospital
2022
Saint Vincent's Catholic Medical Center
2022
Allen Institute for Brain Science
2022
Vanderbilt University
2011-2020
Increasing attention has focused on the role of free radicals derived from oxygen in pathophysiology a wide variety disorders. One well-recognized targets radical-induced injury is peroxidation lipids. Using approaches, we have found that series prostaglandin F2-like compounds are produced vivo humans by non-cyclooxygenase mechanism involving radical-catalyzed arachidonic acid. Levels these normal human plasma and urine range 5 to 40 pg/ml 500 4000 pg/mg creatinine, respectively. In rats,...
It has been hypothesized that the pathogenesis of diseases induced by cigarette smoking involves oxidative damage free radicals. However, definitive evidence causes modification target molecules in vivo is lacking. We conducted a study to determine whether production F2-isoprostanes, which are novel products lipid peroxidation, enhanced persons who smoke.We measured levels F2-isoprostanes plasma, esterified plasma lipids, and urinary excretion metabolites 10 smokers nonsmokers matched for...
To assess the pharmacologic effects of aspirin on endogenous prostacyclin and thromboxane biosynthesis, 2,3-dinor-6-keto PGF1 alpha (PGI-M) 2,3-dinor-thromboxane B2 (Tx-M) were measured in urine by mass spectrometry during continuing administration aspirin. define relationship intake to sequential urines initially collected individuals prior to, during, subsequent Despite inter- intra-individual variations, PGI-M excretion was significantly reduced However, full spectral identification...
Anti-IgE-dependent activation of rat and human mast cells resulted in the preferential generation cyclooxygenase products prostaglandin D2 (PGD2) I2 (PGI2) PGD2 human. The average net PGD2, determined by gas chromatography-mass spectrometry, was 13.1 ng/10(6) purified 39.5 dispersed, enriched cells. After IgE-dependent activation, there a linear relationship between quantities generated histamine secreted from dispersed pulmonary when number varied but total held constant, indicating that it...
OBJECTIVE: The goal was to reduce adverse pulmonary outcomes, oxidative stress, and inflammation in neonates of 24 28 weeks gestation initially resuscitated with fractions inspired oxygen 30% or 90%. METHODS: Randomized assignment receive (N = 37) 90% 41) performed. Targeted saturation values were 75% at 5 minutes 85% 10 minutes. Blood oxidized glutathione (GSSG)/reduced ratio urinary o-tyrosine, 8-oxo-dihydroxyguanosine, isoprostane levels, isofuran elimination, plasma interleukin 8 tumor...
F<sub>2</sub>-isoprostanes are prostaglandin F<sub>2</sub>-like compounds that formed nonenzymatically by free radical-induced oxidation of arachidonic acid. We explored whether docosahexaenoic acid (C22:6ω3), which is highly enriched in the brain, led to formation F<sub>2</sub>-isoprostane-like compounds, we term F<sub>4</sub>-neuroprostanes. Oxidation acid<i>in vitro</i> yielded a series were structurally established be F<sub>4</sub>-neuroprostanes using number mass spectrometric...
Vascular oxidative injury accompanies many common conditions associated with hypertension. In the present study, we employed mouse models excessive vascular production of ROS (tgsm/p22phox mice, which overexpress NADPH oxidase subunit p22phox in smooth muscle, and mice vascular-specific deletion extracellular SOD) have shown that these animals develop collagen deposition, aortic stiffening, renal dysfunction, hypertension age. T cells from tgsm/p22phox produced high levels IL-17A IFN-γ....
Mitochondria abnormalities in skeletal muscle may contribute to frailty and sarcopenia, commonly present patients with chronic kidney disease (CKD). Dysfunctional mitochondria are also a major source of oxidative stress cardiovascular CKD. We tested the hypothesis that mitochondrial structure function worsens severity Mitochondrial volume density, DNA (mtDNA) copy number, BNIP3, PGC1α protein expression were evaluated biopsies obtained from 27 subjects (17 controls 10 CKD stage 5 on...
The highest morbidity worldwide from fish poisoning results the ingestion of spoiled scombroid fish, such as tuna and mackerel, its cause is not clear. Histamine could be responsible, because contain large quantities histamine. Whether histamine causative toxin, however, has remained in question. To address this issue, we investigated whether homeostasis altered poisoned people.