A5005633588- Neuroscience and Neuropharmacology Research
- Alcoholism and Thiamine Deficiency
- Infectious Encephalopathies and Encephalitis
- RNA regulation and disease
- Neural dynamics and brain function
- Biochemical Acid Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Vitamin C and Antioxidants Research
- Genetics and Neurodevelopmental Disorders
- Folate and B Vitamins Research
- Traumatic Brain Injury and Neurovascular Disturbances
- S100 Proteins and Annexins
- Traumatic Brain Injury Research
- Biomarkers in Disease Mechanisms
- Ion channel regulation and function
- Cancer-related molecular mechanisms research
- Chronic Lymphocytic Leukemia Research
- Neurological Disease Mechanisms and Treatments
- RNA Research and Splicing
- Renal Diseases and Glomerulopathies
- Heat shock proteins research
- interferon and immune responses
- Electron Spin Resonance Studies
- Neurogenesis and neuroplasticity mechanisms
- Neurological and metabolic disorders
Nationwide Children's Hospital
2024
Office of Infectious Diseases
2024
University of California, Davis
2024
Cleveland Clinic Lerner College of Medicine
2021-2023
Cleveland Clinic
2022
Palladin Institute of Biochemistry
2006-2022
National Academy of Sciences of Ukraine
2007-2022
Technion – Israel Institute of Technology
2017-2021
Cerner (United States)
2021
University of Haifa
2017-2020
Brain imaging has revealed that the CA1 subregion of hippocampus is hyperactive in prodromal and diagnosed patients with schizophrenia (SCZ), glutamate a driver this hyperactivity. Strikingly, mice deficient synthetic enzyme glutaminase have hypoactivity SCZ-resilience profile, implicating glutamate-metabolizing enzymes. To address further, we examined brain-wide deficit dehydrogenase (GDH), encoded by Glud1, which should lead to excess due reduced metabolism astrocytes. We found...
Recent evidence suggests that alterations in oxidative metabolism induced by thiamine deficiency lead to neuronal cell death. However, the molecular mechanisms underlying this process are still under extensive investigation. Here, we report rat pheochromocytoma PC-12 cells differentiated presence of NGF into neurons undergo apoptosis due caused antagonists - amprolium, pyrithiamine and oxythiamine. Confocal laser scanning fluorescence microscopy revealed annexin V binds after 72 h...
Accumulating evidences suggest that p53 is a key coordinator of cellular events triggered by oxidative stress often associated with the impairment in thiamine metabolism and its functions. However, there are limited data regarding pursuant feedback between transactivation homeostasis. Impairment can be induced experimentally via interference uptake and/or inhibition thiamin pyrophosphate-dependent enzymes using antagonists - amprolium (AM), oxythiamine (OT) or pyrithiamine (PT). We found...
Glutamate Dehydrogenase 1 (GDH), encoded by the Glud1 gene in rodents, is a mitochondrial enzyme critical for maintaining glutamate homeostasis at tripartite synapse. Our previous studies indicate that hippocampus may be particularly vulnerable to GDH deficiency central nervous system (CNS). Here, we first asked whether mice with homozygous deletion of CNS (CNS-Glud1 -/- mice) express different levels hippocampus, and found elevated as well glutamine dorsal ventral increased medial...
Traumatic brain injury (TBI)-induced axonal degeneration leads to acute and chronic neuropsychiatric impairment, neuronal death, accelerated neurodegenerative diseases of aging, including Alzheimer's Parkinson's diseases. In laboratory models, is traditionally studied through comprehensive postmortem histological evaluation integrity at multiple time points. This requires large numbers animals power for statistical significance. Here, we developed a method longitudinally monitor functional...
Stroke is a leading cause of disability in the Western world. Current post-stroke rehabilitation treatments are only effective approximately half patients. Therefore, there pressing clinical need for developing new approaches enhancing recovery process, which requires use appropriate animal models. Here, we demonstrate nonlinear microscopy calcium sensors rat brain to study effects ischemic stroke injury on cortical activity patterns. We longitudinally recorded from thousands neurons labeled...
Two variants at the
It was studied the effect of doxorubicin on HIF system and pro-antioxidant balance neonatal cardiomyocytes as well possibility oxidative stress correcting using curcumin. has been revealed that expression mRNA HIF-1α at a dose 0.5 μM 2.9 ± 0.8 cu, so it decreased by 20% compared to control--3.6 0.7 cu (P < 0.05). The level target gene PDK-1 also significantly (4 times). During incubation with doxorubicin, number live cells 50.4% relative control. And after curcumin together, percentage dead...
OBJECTIVES/GOALS: Vanishing White Matter Disease (VWM), is a childhood neurodegenerative leukodystrophy that presents with motor deficits, neurologic decline, and seizures leading to death.There are no treatments. Herein we investigate adeno-associated virus serotype 9 (AAV9) gene addition therapy for VWM. METHODS/STUDY POPULATION: To serve as baseline disease correction, characterized the severe VWM Eif2b5 I98M murine model clinically relevant readouts including function, gait mapping...
The Arf GTPase-activating protein ArfGAP1 and its brain-specific isoform ArfGAP1B play an important role in neurotransmission. Here we analyzed the distribution of mouse brain. We found high levels dentate gyrus where it displayed especially elevated level polymorph layer (hilus). Importantly, signal follows pathway granular cell axons so-called mossy fibers which extend from to CA3 via stratum lucidum partially oriens. Additionally, identified differential expression isocortex. Thus,...
Traumatic brain injury (TBI)-induced axonal degeneration leads to acute and chronic neuropsychiatric impairment, neuronal death, accelerated neurodegenerative diseases of aging, including Alzheimer's Parkinson's diseases. In laboratory models, is traditionally studied through comprehensive post-mortem histological evaluation integrity at multiple time points. This requires large numbers animals power for statistical significance. Here, we report a new method longitudinally monitor functional...
Підвищений рівень анти-Hsp60 антитіл у сироватці крові вважають ознакою серцевосудинних захворювань, фактором ризику атеросклерозу, патологічних змін дрібних судин головного мозку. Метою дослідження було виявлення хворих з артеріальною гіпертензією (АГ) та клінічно здорових, родинний анамнез яких включав випадки АГ по материнській лінії. Антитіла проти прокаріотичного Hsp60 (GroEL Escherihia coli) людини визначали 39 обстежених. Серед них 12 родинна історія включала лінії (І група), 27...
Abstract Traumatic brain injury-induced axonal degeneration leads to acute and chronic neuropsychiatric impairment, neuronal death, accelerated neurodegenerative diseases of aging, including Alzheimer’s Parkinson’s diseases. Thus, there is much interest in developing treatments that protect axons after injury. For this endeavor, extended comprehensive evaluation integrity experimental systems required evaluate the efficacy putative interventions preclinical models. However, traditional...
Abstract Stroke is a leading cause of disability in the Western world. Current post-stroke rehabilitation treatments are only effective approximately half patients. Therefore, there pressing clinical need for developing new approaches enhancing recovery process, which requires use appropriate animal models. Here we study activity patterns multiple cortical regions rat brain using two-photon microscopy. We longitudinally recorded fluorescence signal from thousands neurons labeled with...