A5032839894- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Computational Drug Discovery Methods
- 14-3-3 protein interactions
- Cancer Cells and Metastasis
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Cancer Treatment and Pharmacology
- 3D Printing in Biomedical Research
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Angiogenesis and VEGF in Cancer
- Advanced Biosensing Techniques and Applications
- Bone health and osteoporosis research
- Monoclonal and Polyclonal Antibodies Research
- Axon Guidance and Neuronal Signaling
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Pancreatic and Hepatic Oncology Research
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Corneal surgery and disorders
- Cancer, Hypoxia, and Metabolism
- Phagocytosis and Immune Regulation
University of California, San Francisco
2020-2024
University of California, Berkeley
2022
California Institute for Regenerative Medicine
2020
Harvey Mudd College
2019
Desmoplasia describes the deposition of extensive extracellular matrix and defines primary pancreatic ductal adenocarcinoma (PDA). The acellular component this stroma has been implicated in PDA pathogenesis is being targeted therapeutically clinical trials. By analyzing stromal content samples from numerous annotated data sets correlating with both anatomic site outcome, we found metastases liver, cause mortality to have less stroma, higher tumor cellularity than tumors. Experimentally...
Abstract Failure to precisely distinguish malignant from healthy tissue has severe implications for breast cancer surgical outcomes. Clinical prognoses depend on distinguishing during surgery. Laser Raman spectroscopy (LRS) been previously shown differentiate benign in real time. However, the cost, assembly effort, and technical expertise needed construction implementation of technique have prohibited widespread adoption. Recently, spectrometers developed non-medical uses become commercially...
Abstract Intratumor heterogeneity associates with poor patient outcome. Stromal stiffening also accompanies cancer. Whether cancers demonstrate stiffness heterogeneity, and if this is linked to tumor cell remains unclear. We developed a method measure the in human breast tumors that quantifies stromal each experiences permits visual registration biomarkers of progression. present S patially T ransformed I nferential F orce Map (STIFMap) which exploits computer vision precisely automate...
The regulation of cell physiology depends largely upon interactions functionally distinct proteins and cellular components. These may be transient or long-lived, but often affect protein motion. Measurement dynamics within a environment, particularly while perturbing function with small molecules, enable dissection key facilitate drug discovery; however, current approaches are limited by throughput respect to data acquisition analysis. As result, studies using super-resolution imaging...
The regulation of cell physiology depends largely upon interactions functionally distinct proteins and cellular components. These may be transient or long-lived, but often affect protein motion. Measurement dynamics within a environment, particularly while perturbing function with small molecules, enable dissection key facilitate drug discovery; however, current approaches are limited by throughput respect to data acquisition analysis. As result, studies using super-resolution imaging...
Tumors feature elevated sialoglycoprotein content. Sialoglycoproteins promote tumor progression and are linked to immune suppression via the sialic acid-Siglec axis. Understanding factors that increase biosynthesis in tumors could identify approaches improve patient response immunotherapy. We quantified higher levels of sialoglycoproteins fibrotic regions within human breast tissues. Human subtypes, which more fibrotic, similarly featured increased Further analysis revealed cancer cells as...
Abstract The regulation of cell physiology depends largely upon interactions functionally distinct proteins and cellular components. These may be transient or long-lived, but often affect protein motion. Measurement dynamics within a environment, particularly while perturbing function with small molecules, enable dissection key facilitate drug discovery; however, current approaches are limited by throughput respect to data acquisition analysis. As result, studies using super-resolution...
The regulation of cell physiology depends largely upon interactions functionally distinct proteins and cellular components. These may be transient or long-lived, but often affect protein motion. Measurement dynamics within a environment, particularly while perturbing function with small molecules, enable dissection key facilitate drug discovery; however, current approaches are limited by throughput respect to data acquisition analysis. As result, studies using super-resolution imaging...
The regulation of cell physiology depends largely upon interactions functionally distinct proteins and cellular components. These may be transient or long-lived, but often affect protein motion. Measurement dynamics within a environment, particularly while perturbing function with small molecules, enable dissection key facilitate drug discovery; however, current approaches are limited by throughput respect to data acquisition analysis. As result, studies using super-resolution imaging...
The construction of three-dimensional (3D) microvascular networks with defined structures remains challenging. Emerging bioprinting strategies provide a means patterning endothelial cells (ECs) into the geometry 3D networks, but microenvironmental cues necessary to promote their self-organization cohesive and perfusable microvessels are not well known. To this end, we reconstituted microvessel formation in vitro by thin lines closely packed ECs fully embedded within extracellular matrix...
Abstract Tumor progression is accompanied by fibrosis, which associated with diminished anti-tumor immune infiltrate. Here, we demonstrate that tumor infiltrating myeloid cells respond to the stiffened fibrotic microenvironment (TME) initiating a TGF-beta (TGFβ)-directed, collagen biosynthesis program. A collateral effect of this programming an untenable metabolic milieu for productive CD8 T cell responses, as collagen-synthesizing macrophages consume environmental arginine, synthesize...
Abstract Mitochondria control eukaryotic cell fate by producing the energy needed to support life and signals required execute programmed death. The biochemical milieu is known affect mitochondrial function contribute dysfunctional phenotypes implicated in cancer morbidities of ageing. However, physical characteristics extracellular matrix are also altered aging tissues. We demonstrate that cells sense properties activate a stress response adaptively tunes via SLC9A1-dependent ion exchange...
Abstract The construction of three-dimensional (3D) microvascular networks with defined structures remains challenging. Emerging bioprinting strategies provide a means patterning endothelial cells (ECs) into the geometry 3D networks, but microenvironmental cues necessary to promote their self-organization cohesive and perfusable microvessels are unknown. To this end, we reconstituted microvessel formation in vitro by thin lines closely packed ECs fully embedded within extracellular matrix...
<title>Abstract</title> Tumor progression is accompanied by fibrosis, which associated with diminished anti-tumor immune infiltrate. Here, we demonstrate that tumor infiltrating myeloid cells respond to the stiffened fibrotic microenvironment (TME) initiating a TGF-beta (TGFβ)-directed, collagen biosynthesis program. A collateral effect of this programming an untenable metabolic milieu for productive CD8 T cell responses, as collagen-synthesizing macrophages consume environmental arginine,...
Abstract Primary tissue organoids and cell spheroids recapitulate physiology with remarkable fidelity. We investigated how engagement a three dimensional laminin-rich extracellular matrix supports the polarized, stress resilient spheroid phenotype of mammary epithelial cells. Cells within decreased redistributed actin crosslinker filamin to reduce their cortical tension. low tension had increased plasma membrane protrusions that promoted negative curvature fostered protein associations...
ABSTRACT Tissue stem-progenitor cell frequency has been implicated in tumor risk and progression. Tissue-specific factors linking to cancer progression remain ill defined. Using a genetically engineered mouse model that promotes integrin mechanosignaling with syngeneic manipulations, spheroid models, patient-derived xenografts we determined stiff extracellular matrix high increase enhance breast Studies revealed integrin-mechanosignaling expands epithelial number by potentiating progesterone...