A5086599953- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Connexins and lens biology
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Retinopathy of Prematurity Studies
- melanin and skin pigmentation
- bioluminescence and chemiluminescence research
- Phosphodiesterase function and regulation
- Neuroscience and Neuropharmacology Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Calcium signaling and nucleotide metabolism
- Biochemical effects in animals
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- RNA regulation and disease
- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- Free Radicals and Antioxidants
- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- Calpain Protease Function and Regulation
Yunnan University
2021-2024
Second People's Hospital of Yunnan Province
2016-2024
STZ eyetrial
2015-2016
University of Tübingen
2015-2016
Kunming Medical University
2015
Renmin Hospital of Wuhan University
2013-2014
Wuhan University
2013-2014
Abstract Inherited retinal degenerations (IRDs) are a group of untreatable and commonly blinding diseases characterized by progressive photoreceptor loss. IRD pathology has been linked to an excessive activation cyclic nucleotide-gated channels (CNGC) leading Na + - Ca 2+ -influx, subsequent voltage-gated -channels (VGCC), further influx. However, connection between influx loss yet be proven. Here, we used whole-retina single-cell RNA-sequencing compare gene expression the rd1 mouse model...
Mutations in the PDE6A gene can cause rod photoreceptors degeneration and blinding disease retinitis pigmentosa (RP). While a number of pathogenic mutations have been described, little is known about their impact on compound heterozygous situations potential interactions different disease-causing alleles. Here, we used novel mouse model for Pde6a R562W mutation combination with an existing line carrying V685M to generate V685M/R562W animals, exactly homologous case human RP. We compared...
The cellular mechanisms underlying hereditary photoreceptor degeneration are still poorly understood. aim of this study was to systematically map the transcriptional changes that occur in degenerating mouse retina at single cell level. To end, we employed single-cell RNA-sequencing (scRNA-seq) and retinal degeneration-1 (rd1) mice profile impact disease mutation on diverse types during early post-natal development. transcriptome data allowed annotate 43,979 individual cells grouped into 20...
Abstract Retinitis pigmentosa (RP), an inherited blinding disease, is caused by a variety of different mutations that affect retinal photoreceptor function and survival. So far there neither effective treatment nor cure. We have previously shown poly(ADP-ribose)polymerase (PARP) acts as common critical denominator cell death in photoreceptors, qualifying it potential target for future therapeutic intervention. A significant fraction RP-causing the genes rod phosphodiesterase 6A (PDE6A)...
Abstract The retina has the highest relative energy consumption of any tissue, depending on a steady supply glucose from bloodstream. Glucose uptake is mediated by specific transporters whose regulation and expression are critical for pathogenesis many diseases, including diabetes diabetic retinopathy. Here, we used immunofluorescence to show that transporter‐2 (GLUT2) expressed in horizontal cells mouse neuroretina proximity inner retinal capillaries. To study function GLUT2 murine retina,...
Retinitis pigmentosa (RP) is a group of inherited retinal dystrophies that typically results in photoreceptor cell death and vision loss. Here, we explored the effect early growth response-1 (EGR1) expression on Pde6brd1 (rd1) mice its mechanism action. To this end, single-cell RNA-seq (scRNA-seq) was used to identify differentially expressed genes rd1 congenic wild-type (WT) mice. Chromatin immunoprecipitation (ChIP), dual-luciferase reporter gene assay, western blotting were verify...
Inherited retinal degenerations (IRDs) are a group of blinding diseases, typically involving progressive loss photoreceptors. The IRD pathology is often based on an accumulation cGMP in photoreceptors and associated with the excessive activation calpain poly (ADP-ribose) polymerase (PARP). Inhibitors or PARP have shown promise preventing photoreceptor cell death, yet relationship between these enzymes remains unclear. To explore this further, organotypic explant cultures derived from...
This research aimed to ascertain the neuroprotective effect of histone deacetylase (HDAC) inhibition on retinal photoreceptors in Pde6brd1 mice, a model retinitis pigmentosa (RP).Single-cell RNA-sequencing (scRNA-seq) explored HDAC and poly (ADP-ribose) polymerase (PARP)-related gene expression both Pde6b-mutant rd1 wild-type (WT) mice. The CUT&Tag method was employed examine functions Organotypic explant cultures from WT mice were exposed inhibitor SAHA (suberoylanilide hydroxamic acid)...
Purpose: The present work investigated changes in the gene expression, molecular mechanisms, and pathogenesis of inherited retinal degeneration (RD) three different disease models, to identify predictive biomarkers for their varied phenotypes provide a better scientific basis diagnosis, treatment, prevention. Methods: Differentially expressed genes (DEGs) between tissue from RD mouse models obtained during photoreceptor cell death peak period (Pde6brd1 at post-natal (PN) day 13, Pde6brd10...
Inherited retinal degeneration (IRD) represents a diverse group of gene mutation-induced blinding diseases. In IRD, the loss photoreceptors is often connected to excessive activation histone-deacetylase (HDAC), poly-ADP-ribose-polymerase (PARP), and calpain-type proteases (calpain). Moreover, inhibition either HDACs, PARPs, or calpains has previously shown promise in preventing photoreceptor cell death, although relationship between these enzyme groups remains unclear. To explore this...
Astrocyte patterns affect the normal development of retinal vascular network in retinopathy prematurity (ROP), which is associated with VEGF secretion. However, role astrocyte polarization this process remains unknown. Therefore, study aimed to track status A1/A2 reactive astrocytes retinas oxygen-induced (OIR) model and their association expression. The C57BL/6 mouse OIR was constructed characterize pathological changes ROP. Immunofluorescence iB4 GFAP staining performed observe pattern at...
Hereditary retinal degeneration (RD) is characterized by progressive photoreceptor cell death. Overactivation of the cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG) pathway in cells causes death, especially models harboring phosphodiesterase 6b (PDE6b) mutations. Previous studies on RD have used mainly murine such as rd1 or rd10 mice. Given genetic and physiological differences between mice humans, it important to understand which extent retinas primates rodents are...
Objective To study the relationship between expression of CREB1 and retinal neovascularization (RNV) in a mouse model oxygen-induced retinopathy (OIR).Methods Postnatal day 7 (P7) mice (n=134) were randomly assigned to two groups:a control group (n=67) an OIR (n=67).OIR was induced by exposing P7 (75±2)％ O2 for 5 days,followed exposure room air additional days.P17 raised normal environment 4 days.The from 21 P17 groups sacrificed,and sections HE staining flat mounts after cardiac perfusion...
Objective To investigate the clinical features of ocular injuries due to chopping fire wood and potential prognostic indicators for vision.Methods This is a retrospective analysis 208 patients (210 eyes) with wood-related injuries.Age,gender,laterality,type wood,location incident,initial best-corrected visual acuity (BCVA),diagnosis,management,and final BCVA at last follow-up were documented.Relevant outcome evaluated.Results We observed 210 eyes in patients.Injuries more frequent males (186...
ABSTRACT Inherited retinal degeneration (IRD) refers to a group of untreatable blinding diseases characterized by progressive loss photoreceptors. IRD pathology is often linked an excessive activation cyclic nucleotide-gated channels (CNGC) leading Na + – and Ca 2+ -influx, subsequent voltage-gated -channels (VGCC), further influx. However, whether how exactly intracellular overload contributes photoreceptor still controversial. Here, we used whole-retina single-cell RNA-sequencing compare...
Abstract The cellular mechanisms underlying hereditary photoreceptor degeneration are still poorly understood. aim of this study was to systematically map the transcriptional changes that occur in degenerating mouse retina at single cell level. To end, we employed RNA-sequencing (scRNA-seq) and retinal degeneration-1 ( rd1 ) mice profile impact disease mutation on diverse types during early post-natal development. transcriptome data allowed annotate 43,979 individual cells grouped into 20...
Abstract Purpose : Inhibition of PARP1 could relieve PDE6 mutation-induced Retinitis pigmentosa (RP). However, the mechanism related with overexpression in RP has not been clarified. We attempted to explore potential regulatory underlying RP. Methods ATAC-seq and RNA-seq were preformed for retina tissues C3H rd1 mice. The differential expressed genes (DEGs) identified, followed by PARP1-DEG coexpression network, PPI network construction. GO-BP pathway enrichment DEGs interest performed...