A5101608013- Retinal Diseases and Treatments
- Retinopathy of Prematurity Studies
- Angiogenesis and VEGF in Cancer
- Galectins and Cancer Biology
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- Traumatic Brain Injury and Neurovascular Disturbances
- Retinal Development and Disorders
- Monoclonal and Polyclonal Antibodies Research
- Autophagy in Disease and Therapy
- Prostate Cancer Treatment and Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Glaucoma and retinal disorders
- Ocular Oncology and Treatments
- Neonatal and fetal brain pathology
- RNA modifications and cancer
- Inflammation biomarkers and pathways
- Connexins and lens biology
- Intensive Care Unit Cognitive Disorders
- Sirtuins and Resveratrol in Medicine
- Retinoids in leukemia and cellular processes
- Protein Tyrosine Phosphatases
- HER2/EGFR in Cancer Research
- Signaling Pathways in Disease
- Click Chemistry and Applications
Wuhan University
2012-2025
Renmin Hospital of Wuhan University
2010-2025
Guangzhou Medical University
2024
Third Affiliated Hospital of Guangzhou Medical University
2024
Key Laboratory of Guangdong Province
2024
Shanghai Micro Electronics Equipment (China)
2023
Pfizer (United States)
2015-2022
Dalian Medical University
2022
Chinese PLA General Hospital
2017-2020
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2020
Bispecific antibodies offer a promising approach for the treatment of cancer but can be challenging to engineer and manufacture. Here we report development PF-06671008, an extended-half-life dual-affinity re-targeting (DART®) bispecific molecule against P-cadherin CD3 that demonstrates antibody-like properties. Using phage display, identified anti-P-cadherin single chain Fv (scFv) were subsequently affinity-optimized picomolar affinity using stringent selection strategies, resulting in low...
Abstract Background Polyploid giant cancer cells (PGCCs) contribute to solid tumor heterogeneity. This study investigated the relationships among PGCCs numbers, vasculogenic mimicry (VM) formation, and grades in glioma. Methods A total of 76 paraffin-embedded glioma tissue samples, including 28 cases low grade 48 high gliomas, were performed with H&E immunohistochemical staining for Ki-67 hemoglobin. The size nuclei was measured by a micrometer using section defined as at least three...
The expression of human SLFN11 was reported to sensitize cancer cells DNA damaging agents. This study is explore the epigenetic change and function in colorectal (CRC).Six CRC cell lines 128 primary samples were used.SLFN11 methylated 55.47% (71/128) CRC. regulated by promoter region methylation. Methylation significantly associated with age, poor 5-year overall survival relapse-free (all p < 0.05). suppressed growth both vitro vivo sensitized cisplatin.SLFN11 frequently CRC, reduced...
Diabetic retinopathy, a severe complication of diabetes, is the leading cause blindness in developed world. This study investigated effects quercetin on levels monocyte chemoattractant protein-1 (MCP-1), matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) serum rats with diabetic explored functional mechanisms treatment retinopathy. Twenty induced diabetes were divided into model group group, 10 each group. Ten healthy also included to serve as control Rats...
Purpose: N6-methyladenosine (m6A) modification has been implicated in ischemia–reperfusion injury various systems and several neurodegenerative diseases. Glaucoma is characterized by degeneration of retinal ganglion cells (RGCs) shares similar pathologic characteristics with (RIR) injury. However, the specific role m6A RIR unclear, involvement autophagy also remains controversial. Therefore, our study explored Methods: Male wild-type C57BL/6J mice (6–8 weeks old) were used to induce Retinal...
Abstract BACKGROUND Castration‐resistant prostate cancer (CRPC) represents a therapeutic challenge for current medications. METHODS In order to explore the molecular mechanisms involved in CRPC progression and identify new targets, we analyzed unique sample set of 11 CRPCs 7 advanced tumors by array‐CGH gene expression microarrays. The genome‐wide DNA RNA data were integrated genes whose overexpression was driven their amplification. To assess functional role these genes, transcriptional 329...
Abstract Next-generation site-specific cysteine-based antibody–drug-conjugates (ADCs) broaden therapeutic index by precise drug-antibody attachments. However, manufacturing such ADCs for clinical validation requires complex full reduction and reoxidation processes, impacting product quality. To overcome this technical challenge, we developed a novel antibody process through cysteine (Cys) metabolic engineering in Chinese hamster ovary cells implementing unique cysteine-capping technology....
Diabetic retinopathy (DR) is a serious symptom associated with diabetes and could cause much suffer to patients. MiR-221, SIRT1 Nrf2 were apoptosis proliferation their expression altered in DR However, roles regulatory mechanisms human retinal microvascular endothelial cells (hRMEC) not clear.Expression of mRNA was detected by qRT-PCR. Protein Western blot. Interaction between miR-221 predicted bioinformatics analysis validated dual-luciferase reporter assay. We analyzed the viability hRMEC...
Previous studies provided evidence that many factors contribute to retinal angiogenesis, including inducible nitric oxide synthase (iNOS), hypoxia-inducible factor-1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF). But the role of generated by iNOS in regulation expression genes retinopathy prematurity remains unclear. So we sought better define molecular basis this iNOS-dependent regulation.In study, using immunohistochemistry, real-time PCR Western blotting technologies,...
In Brief Objectives There is a clear need for better therapeutics and diagnostics pancreatic cancer. We aimed to discover plasma membrane-associated proteins overexpressed in cancer using quantitative proteomics apply RNA interference (RNAi) uncover associated with cell survival. Methods Cell surface glycoproteins from 5 lines were isolated, differential analyses performed mass spectrometry the “normoid” line Hs766T as comparator. For validation, immunohistochemistry was on tissues 10...
15-lipoxygenase-1 (15-LOX-1) plays an important role in angiogenesis, but how it works still remains a controversial subject. The aims of our study are focused on determining whether or not 15-LOX-1 inhibiting oxygen-induced ischemic retinal neovascularization (RNV) and the underlying regulatory mechanism involving 15-LOX-1, peroxisome proliferator-activated receptor γ (PPAR-γ) vascular endothelial growth factor 2 (VEGFR-2) retinopathy (OIR). Recombinant adenoviral vectors that expressing...
Abstract To investigate the role and potential mechanism of serine/threonine kinase 36 (STK36) in docetaxel resistance-prostate cancer (PCa). The expression STK36 PCa correlation with clinicopathological characteristics patients were analyzed using data from different databases tissue microarrays. on cell proliferation, invasion, migration, was overexpressed silenced DU-145 PC-3 lines. Cell counting kit-8 (CCK8) used to test proliferation. invasion migration detected by wound scratch assay...
Galectin-1 (Gal-1) has been proved to be an important factor in the process of tumor angiogenesis recently. As a small molecule, OTX008 serves as selective inhibitor Gal-1. In this study, role Gal-1 and antiangiogenic effect on retinal neovascularization (RNV) were investigated using mouse model oxygen-induced retinopathy. The outcome indicated that was overexpressed closely related neovessels OIR. After intravitreal injection at P12, RNV significantly reduced P17, measuring by...