A5101563674- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Gut microbiota and health
- Cytomegalovirus and herpesvirus research
- Metabolism, Diabetes, and Cancer
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Immune responses and vaccinations
- Malaria Research and Control
- Diet and metabolism studies
- Medical Research and Treatments
- vaccines and immunoinformatics approaches
- Glycogen Storage Diseases and Myoclonus
- Digestive system and related health
- Muscle metabolism and nutrition
- GABA and Rice Research
- Probiotics and Fermented Foods
- Opinion Dynamics and Social Influence
- Head and Neck Cancer Studies
- Antimicrobial Peptides and Activities
- Lipid metabolism and biosynthesis
- Enhanced Recovery After Surgery
- Endoplasmic Reticulum Stress and Disease
Peter Doherty Institute
2016-2025
The University of Melbourne
2016-2025
Peking University
2023-2025
Wuhan Children's Hospital
2021-2025
Huazhong University of Science and Technology
2013-2025
Carnegie Mellon University
2024
Australian Research Council
2019-2021
West China Hospital of Sichuan University
2018
Sichuan University
2018
ARC Centre of Excellence for Plant Success in Nature and Agriculture
2013-2016
Mucosal associated invariant T (MAIT) cells recognise conserved microbial metabolites from riboflavin synthesis. Striking evolutionary conservation and pulmonary abundance implicate them in antibacterial host defence, yet their functions protection against clinically important pathogens are unknown. Here we show that mouse Legionella longbeachae infection induces MR1-dependent MAIT cell activation rapid accumulation of with immune detectable immunocompetent animals. is more evident mice...
A different way for γδ T cells to bind The ligands bound by cell receptors (TCRs) are less well characterized than those of their αβ TCR cousins, which antigens presented major histocompatibility complex (MHC) and related proteins. Le Nours et al. identified a phenotypically diverse subset in human tissues that reacts MHC-related protein 1 (MR1), presents vitamin B derivatives. crystal structure TCR–MR1–antigen revealed some these TCRs can underneath the MR1 antigen-binding cleft instead...
Abstract Gut microbiota and their metabolic products might play important roles in regulating the pathogenesis of autism spectrum disorder (ASD). The purpose this study was to characterize gut serum amino acid metabolome profiles children with ASD. A non-randomized controlled carried out analyze alterations intestinal metabolites patients ASD (n = 30) compared neurotypical controls (NC) by metagenomic sequencing define community liquid chromatography/mass spectrometry (LC/MS) analysis...
Recent clinical studies have demonstrated a beneficial effect of Saccharomyces boulardii (S. boulardii) in inflammatory bowel disease (IBD). However, the underlying mechanisms remain poorly defined. In this study, we investigated modulating S. on intestinal microbiota humanized mice with dextran sulfate sodium (DSS)-induced colitis. The were fed an boulardii-supplement diet for 16 days before DSS treatment. results showed that feeding significantly ameliorated colon damage and regulated...
Mucosal-associated invariant T (MAIT) cells detect microbial infection via recognition of riboflavin-based antigens presented by the major histocompatibility complex class I (MHC-I)–related protein 1 (MR1). Most MAIT in human peripheral blood express CD8αα or CD8αβ coreceptors, and binding site for CD8 on MHC-I molecules is relatively conserved MR1. Yet, there no direct evidence interacting with MR1 functional consequences thereof. Similarly, role lymphocyte function remains ill-defined....
The antigen-presenting molecule MR1 (MHC class I-related protein 1) presents metabolite antigens derived from microbial vitamin B
Mucosal-associated invariant T (MAIT) cells, natural killer (NKT) and γδT cells are collectively referred to as 'unconventional cells' due their recognition of non-peptide antigens restriction MHC-I-like molecules. However, the factors controlling widely variable frequencies between individuals organs poorly understood. We demonstrated that MAIT increased in NKT or cell-deficient mice highly expand lacking both cell types. TCRα repertoire analysis thymocytes revealed altered Trav segment...
Naive CD4+ T cells must differentiate in order to orchestrate immunity Plasmodium, yet understanding of their emerging phenotypes, clonality, spatial distributions, and cellular interactions remains incomplete. Here, we observe that splenic polyclonal toward helper 1 (Th1) follicular (Tfh)-like states exhibit rarer phenotypes not elicited among cell receptor (TCR) transgenic counterparts. TCR clones present at higher frequencies Th1 skewing, suggesting variation major histocompatibility...
Liver glycogen, a highly branched polymer of glucose, is important for maintaining blood-glucose homeostasis. It was recently shown that db/db mice, model Type 2 diabetes, are unable to form the large composite glycogen α particles present in normal, healthy mice. In this study, structure mouse-liver over diurnal cycle characterized using size exclusion chromatography and transmission electron microscopy. Glycogen found be formed as smaller β particles, then only assembled into with broad...
Plasmodium- specific CD4 + T cells differentiate into effector and memory subsets during experimental malaria, via mechanisms that remain incompletely characterised. By mining scRNA-seq data of Plasmodium chabaudi AS infection in mice, we identified two genes previously uncharacterised helper cells, long-tailed unconventional myosin 1f ( Myo1f ) proline-rich13/taxanes-resistance 1 Prr13 / Txr1 ), which were upregulated differentiation. is reported to regulate motility granule exocytosis...
Human pregnancy presents a unique physiological state that allows for growth of an antigenically dissimilar foetus and requires specific adaptations the immune system. The system plays important role in establishing maintaining successful pregnancy, yet deep understanding immunological responses is lacking. To provide in-depth landscape with focus on NK cells, we used high-throughput cell surface proteome screening >350 markers scRNAseq 130 CITE-seq antibodies to identify key...
Abstract Children in malaria-endemic regions can experience repeated Plasmodium infections over short periods of time. Effects re-infection on multiple co-existing CD4 + T cell subsets remain unresolved. Here, we examine antigen-experienced cells during mice, using scRNA-seq/TCR-seq and spatial transcriptomics. TCR transgenic EM initiate rapid Th1/Tr1 recall responses prior to proliferating, while GC Tfh counterparts are refractory, with CM /Tfh-like exhibiting modest non-proliferative...
Mucosal-associated invariant T (MAIT) cells are known for their rapid effector functions and antibacterial immune protection. Here, we define the plasticity of interferon-γ (IFN-γ)–producing MAIT1 interleukin-17A (IL-17A)–producing MAIT17 cell subsets in vivo. Whereas T-bet + remained stable all experimental settings, after adoptive transfer or acute Legionella Francisella infection, RORγt could undergo phenotypic functional conversion into both MAIT1/17 − cells. This ensured that played a...
CD1 molecules bind CD36 family members, and blocking this interaction facilitates staining study of CD1-restricted T cells.
Significance Whereas T cells are known to recognize peptides, vitamin B metabolites, or lipid antigens, we identify several nonlipidic small molecules classified as pentamethylbenzofuransulfonates (PBFs) that activate a population of CD1d-restricted natural killer (NKT) cells. This represents breakthrough in the field NKT cell biology. study also reveals previously unknown PBF-reactive healthy individuals with stereotyped receptors paves way for future studies role these immunity, including...
Abstract Mucosal‐associated invariant T (MAIT) cells are a subset of unconventional restricted by the major histocompatibility complex (MHC) class I–like molecule MHC‐related protein 1 (MR1). MAIT found throughout body, especially in human blood and liver. Unlike conventional cells, which stimulated peptide antigens presented MHC molecules, recognize metabolite derived from an intermediate microbial biosynthesis riboflavin. mediate protective immunity to infections riboflavin‐producing...
Lipoprotein assembly and secretion in the small intestine are critical for dietary fat absorption. Surfeit locus protein 4 (SURF4) serves as a cargo receptor, facilitating cellular transport of multiple proteins mediating hepatic lipid vivo. However, its involvement intestinal is not fully understood. In this study, we investigated role SURF4
Abstract Butyrophilin (BTN) molecules are emerging as key regulators of T cell immunity, however, how they trigger cell-mediated responses is poorly understood. Here, the crystal structure a gamma-delta receptor (γδTCR) in complex with BTN member 2A1 (BTN2A1) revealed that BTN2A1 engages side γδTCR, leaving apical TCR surface bioavailable. We reveal BTN3A1 second γδTCR ligand, co-engages via binding to this accessible surface. and also directly interact each other cis, structural analysis...