A5087487872- Reproductive tract infections research
- Microbial infections and disease research
- Blood groups and transfusion
- Syphilis Diagnosis and Treatment
- Bacteriophages and microbial interactions
- Urinary Tract Infections Management
- Genital Health and Disease
- Herpesvirus Infections and Treatments
- HIV, TB, and STIs Epidemiology
- HIV, Drug Use, Sexual Risk
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Fungal and yeast genetics research
- Neuropeptides and Animal Physiology
- Plant Virus Research Studies
- Health, Medicine and Society
- Urological Disorders and Treatments
- Polyamine Metabolism and Applications
- Plant Disease Resistance and Genetics
- Pharmaceutical Economics and Policy
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- Pelvic floor disorders treatments
- Bacterial Identification and Susceptibility Testing
Université de Bordeaux
2014-2023
Centre National de la Recherche Scientifique
2007-2023
Institut Polytechnique de Bordeaux
2012-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2011-2021
Centre Hospitalier Universitaire de Bordeaux
2012-2019
Université Rennes 2
2018
Université de Rennes
2018
Institut National de la Recherche Agronomique du Niger
2012-2017
Hôpital de la Timone
2016
Maastricht University Medical Centre
2015
As information on Ureaplasma spp. and Mycoplasma hominis resistance is currently limited, the aim of this study was to investigate susceptibility M. tetracyclines fluoroquinolones in France.The 1014 clinical isolates obtained Bordeaux University Hospital (Bordeaux, France) between 2010 2015 evaluated using two commercial kits, S.I.R. (Bio-Rad) from 1 January 5 October 2012 MYCOFAST RevolutioN kit (ELITech Group) 6 31 December 2015. The MICs designated as resistant were determined broth...
Remnant specimens from 601 women obtained in a cross-sectional study rural South Africa were tested for Mycoplasma genitalium. Overall, 10.8% of infected with M. genitalium either the vagina or rectum. Macrolide resistance, although low prevalence, is described first time Sub-Saharan Africa.
Mycoplasma genitalium (MG) is an emerging pathogen among men who have sex with (MSM) raising rates of antibiotic resistance. This study assessed the prevalence and incidence MG infection in MSM enrolled open-label phase ANRS IPERGAY trial on-demand tenofovir disoproxil fumarate/emtricitabine for human immunodeficiency virus prevention impact doxycycline post-exposure prophylaxis (PEP).210 subjects were tested at baseline 6 months by real-time PCR assays detection urine samples oropharyngeal...
The increasing frequency of macrolide resistance is an emerging issue in the treatment Mycoplasma genitalium infection. Because evaluation new commercial kits detecting M. and needed, we evaluated performance handling characteristics Allplex MG & AziR (Seegene), Macrolide-R/MG ELITe MGB (ELITechGroup), ResistancePlus FleXible (SpeeDx-Cepheid) comparison with those in-house real-time PCR 23S rRNA gene sequencing used as reference. A total 239 urogenital specimens (135 genitalium-positive 104...
The aim of this study was to evaluate the clinical performance Aptima Mycoplasma genitalium transcription-mediated amplification (MG-TMA) CE-marked for in vitro diagnosis (CE-IVD) assay detection male and female samples comparison with in-house real-time PCR (in-house PCR) routinely used our laboratory. A total 1,431 specimens obtained from 1,235 patients were prospectively collected at Bacteriology Department Bordeaux University Hospital (France). Additional research-use-only M. (TMA)...
As macrolide resistance in Mycoplasma genitalium is increasing worldwide, resistance-associated mutations should be assessed M. genitalium-positive specimens. New commercial kits are available for detection of concurrently with We prospectively evaluated the handling and clinical performances three Between August December 2018, remnants all urogenital specimens determined to positive using an in-house real-time PCR assay were collected at French National Reference Center Bacterial Sexually...
Mycoplasma genitalium causes sexually transmitted infecti.ons in men and women. Treatment failures to macrolides fluoroquinolones have been reported worldwide. Although the mgpB typing method has often used M. genitalium-infected who sex with (MSM), limited data are available for In this study, we aimed investigate genetic relationship between strains their antibiotic resistance profile a cohort of MSM (86.2% on HIV preexposure prophylaxis [PrEP], 13.8% positive) large women using mgpB/MG309...
Tetracyclines are widely used for the treatment of bacterial sexually transmitted infections (STIs) and recently have been successfully post-exposure prophylaxis STIs in MSM. We investigated vitro vivo development tetracycline resistance Chlamydia trachomatis Mycoplasma genitalium evaluated 16S rRNA mutations associated with acquired other bacteria.In selection resistant mutants reference strains C. M. was undertaken by serial passage medium containing subinhibitory concentrations or...
This study describes a new multilocus variable number tandem-repeat (VNTR) analysis (MLVA) typing system for the discrimination of Chlamydia trachomatis genovar D to K isolates or specimens. We focused our MLVA scheme on E which predominates in most populations worldwide. does not require culture and therefore can be performed directly DNA extracted from positive clinical Our method was based GeneScan five VNTR loci labelled with fluorescent dyes by multiplex PCR capillary electrophoresis....
Abstract Objectives Men engaged in high-risk sexual behaviour, such as MSM, are likely to be infected by resistant Mycoplasma genitalium strains. Understanding the transmission dynamics is challenging. We aimed investigate molecular epidemiology of M. men visiting sexually transmitted infection (STI) clinics. Patients and methods Between June 2017 February 2018, 95 genitalium-positive specimens from 78 men, including 76.9% two STI clinics Montpellier, France, were analysed for SNPs mgpB...
The performance of two commercial real-time PCR kits for the detection Mycoplasma genitalium was evaluated in comparison to an in-house assay. Concordances 96% and 93% were found TIB MOLBIOL Diagenode assays, respectively, compared results
Mycoplasma hominis is a minimal human pathogen that responsible for genital and neonatal infections. Despite many attempts, there no efficient genetic tool to manipulate this bacterium, limiting most investigations of its pathogenicity uncommon energy metabolism relies on arginine. The recent cloning subsequent engineering other mycoplasma genomes in yeast opens new possibilities studies the genetically intractable organisms. Here, we report successful one-step M. PG21 genome using...
Mycoplasma hominis lacks a cell wall, and lipoproteins anchored to the extracellular side of plasma membrane are in direct contact with host components. A Triton X-114 extract M. enriched was shown stimulate production interleukin-23 (IL-23) by human dendritic cells (hDCs). The inflammasome activation has never been reported upon infection. We studied here interaction between PG21 hDCs analyzing both inflammation-inducing mycoplasmal cell. IL-23-inducing were determined using sequential...
Abstract Mycoplasma hominis is an opportunistic human pathogen associated with genital and neonatal infections. Until this study, the lack of a reliable transformation method for genetic manipulation M . hindered investigation pathogenicity peculiar arginine-based metabolism bacterium. A genomic analysis 20 different strains revealed number putative restriction-modification systems in species. Despite presence these systems, reproducible polyethylene glycol (PEG)-mediated protocol was...
To assess the lipoproteins that are involved in interaction between Mycoplasma hominis and human dendritic cells.The surface lipoproteome of M. PG21 was characterized by using Triton X-114 extraction LC-MS/MS identification. The transcriptional changes lipoprotein genes upon contact with cells were determined reverse transcription quantitative PCR after identification reference suitable for normalization.A large-scale overexpression observed 21 upregulated transcripts. Seven unknown function...