A5076398400- Reproductive tract infections research
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Syphilis Diagnosis and Treatment
- Blood groups and transfusion
- HIV, TB, and STIs Epidemiology
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- Herpesvirus Infections and Treatments
- Antibiotic Resistance in Bacteria
- Infectious Disease Case Reports and Treatments
- Bacterial biofilms and quorum sensing
- Urinary and Genital Oncology Studies
- Cervical Cancer and HPV Research
- Antimicrobial Resistance in Staphylococcus
- Bacterial Infections and Vaccines
- Genital Health and Disease
- Bacteriophages and microbial interactions
- Urinary Tract Infections Management
- Mycobacterium research and diagnosis
- Veterinary medicine and infectious diseases
- Pelvic floor disorders treatments
- Diagnosis and treatment of tuberculosis
- Escherichia coli research studies
- Immunotherapy and Immune Responses
Centre Hospitalier Universitaire de Bordeaux
2018-2025
Université de Bordeaux
2012-2023
Saint Louis University Hospital
2016
Assistance Publique – Hôpitaux de Paris
2016
Hôpitaux Universitaires Paris-Ouest
2016
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2012
Institut National de la Recherche Agronomique du Niger
2012
Institut Pasteur de Tunis
2011
Engie (United States)
2006
Mycoplasma genitalium is a sexually transmitted organism commonly treated with azithromycin. However, macrolide resistance has been reported and associated point mutations in the 23S rRNA gene. To evaluate prevalence of M. isolates from clinical specimens France, we first used previously high-resolution melting assay. Because susceptible resistant were hardly discriminated genitalium-positive specimens, developed new molecular assay for rapid detection resistance. An using real-time PCR...
Background In France, lymphogranuloma venereum (LGV) testing switched from universal to selective in 2016. Aim To investigate changes LGV-affected populations, we performed a nationwide survey based on temporarily reinstated LGV 2020 2022. Methods Each year, during three consecutive months, laboratories voluntarily sent anorectal Chlamydia trachomatis -positive samples men and women the National Reference Centre for bacterial sexually transmitted infections. We collected patients’...
Mycoplasma pneumoniae infections occur both endemically and epidemically, macrolide resistance has been spreading for 10 years worldwide. A substantial increased incidence of M. reported in several countries since 2010. Whether this is attributed to different or the same genotype unknown. We have developed a multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) molecular typing isolates. In study, MLVA method was modified validated be applicable directly respiratory tract specimens...
We describe a change in the molecular epidemiology of Chlamydia trachomatis strains involved an outbreak rectal lymphogranuloma venereum France during January 2010-April 2015. Until 2012, C. L2b strain predominated; however, starting 2013, most cases L2 strain. also identified 4 genetic ompA variants.
The performances of five commercial TaqMan real-time PCR assays for the detection Mycoplasma pneumoniae in respiratory tract specimens were evaluated comparison with an in-house PCR. All kits allowed prompt and specific results, validated by use internal control. Nanogen kit showed best clinical sensitivity.
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from whole-genome sequencing eight M. strains, using SNaPshot minisequencing assay. Eight SNPs, localized in housekeeping genes, predicted lipoproteins, adhesin P1 genes were genotyping. These SNPs evaluated 140 clinical isolates previously genotyped by...
Objectives We evaluated the prevalence of lymphogranuloma venereum (LGV) in anorectal Chlamydia trachomatis -positive French men who have sex with (MSM) using pre-exposure prophylaxis (PrEP) for HIV. Here, we describe clinical, biological and behavioural characteristics these patients. Methods Laboratories throughout metropolitan areas performing routine testing C. sent positive specimens to National Reference Centre bacterial STIs LGV real-time PCR targeting pmp H gene. Identification...
Tetracyclines are widely used for the treatment of bacterial sexually transmitted infections (STIs) and recently have been successfully post-exposure prophylaxis STIs in MSM. We investigated vitro vivo development tetracycline resistance Chlamydia trachomatis Mycoplasma genitalium evaluated 16S rRNA mutations associated with acquired other bacteria.In selection resistant mutants reference strains C. M. was undertaken by serial passage medium containing subinhibitory concentrations or...
It is of international priority to develop a vaccine against sexually transmitted Chlamydia trachomatis infections combat the continued global spread infection. The optimal immunization strategy still remains be fully elucidated. aim this study was evaluate strategies in nonhuman primate (NHP) model. Cynomolgus macaques ( Macaqua fascicularis ) were immunized following different multi-component prime-boost immunization-schedules and subsequently challenged with C. SvD lower genital tract....
Thirty-seven Salmonella enterica isolates obtained from poultry meat in Tunisia were included this study for characterization of antibiotic resistance mechanisms. High percentages detected to ampicillin, sulfonamides, tetracycline, nalidixic acid, and streptomycin (32.4%-89.2%), lower amoxicillin-clavulanic kanamycin, amikacin, trimethoprim-sulfamethoxazol, chloramphenicol (2.7%-18.9%). All strains showed susceptibility ceftazidime, cefotaxime, gentamicin, ciprofloxacin. Class 1 integrons...
We evaluated the utility of commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum, herpes simplex virus 1 (HSV-1) and 2 (HSV-2), Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal ulcers context suspected syphilis. In total, 374 documented from patients with without syphilis presenting at several sexually transmitted infection (STI) centers France October 2010 to December 2016 were analyzed National Reference Center (CNR)...
Objectives To describe a series of extrarectal lymphogranuloma venereum (LGV) cases diagnosed in France. Methods Consecutive LGV confirmed at the French Reference Centre for chlamydiae with an sample from January 2010 to December 2015 were included. The first part study consisted retrospective case note review and analysis. In second part, complete omp A gene sequence our samples was determined. Results There 56 overall: 50 genital six pharyngeal LGV. Subjects all men, median age 39 years,...
To assess the lipoproteins that are involved in interaction between Mycoplasma hominis and human dendritic cells.The surface lipoproteome of M. PG21 was characterized by using Triton X-114 extraction LC-MS/MS identification. The transcriptional changes lipoprotein genes upon contact with cells were determined reverse transcription quantitative PCR after identification reference suitable for normalization.A large-scale overexpression observed 21 upregulated transcripts. Seven unknown function...
The objective of this article is to describe the epidemiology lymphogranuloma venereum (LGV) and non-LGV Chlamydia trachomatis anorectal infections in France examine characteristics affected populations via a voluntary sentinel surveillance system for LGV between 2010 2015. Anorectal samples positive C. (CT) were sent by participating laboratories National Reference Center CT identification. Biological clinical data collected biologists clinicians. There 1740 episodes 2248 episodes....