A5025819708- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Sex work and related issues
- HIV, TB, and STIs Epidemiology
- Immunodeficiency and Autoimmune Disorders
- Female Genital Mutilation/Cutting Issues
- Brucella: diagnosis, epidemiology, treatment
- Cervical Cancer and HPV Research
- Bacterial Infections and Vaccines
- HIV/AIDS oral health manifestations
- HIV, Drug Use, Sexual Risk
- Healthcare Systems and Practices
- scientometrics and bibliometrics research
- Parvovirus B19 Infection Studies
- Economic Theory and Policy
- Cytomegalovirus and herpesvirus research
- Pneumonia and Respiratory Infections
- Reproductive Health and Contraception
- Respiratory viral infections research
- Management, Economics, and Public Policy
- Ocular Diseases and Behçet’s Syndrome
- Autoimmune Bullous Skin Diseases
Assistance Publique – Hôpitaux de Paris
2012-2024
Hôpital Cochin
2011-2024
Institut Cochin
2016-2023
Université Paris Cité
2015-2023
Inserm
2020-2023
Centre National de la Recherche Scientifique
2020-2023
Groupe Hospitalier Cochin - Port-Royal, Hôtel-Dieu, Broca - La Collégiale
2013-2023
Sorbonne Université
2016-2020
Service de la Santé Publique
2016
Masaryk University
2016
ABSTRACT Syphilis diagnosis is based on clinical observation, serological analysis, and dark-field microscopy (DFM) detection of Treponema pallidum subsp. , the etiological agent syphilis, in skin ulcers. We performed a nested PCR (nPCR) assay specifically amplifying tpp47 gene T. from swab blood specimens. studied cohort 294 patients with suspected syphilis 35 healthy volunteers. Eighty-seven had primary 103 secondary 40 latent 64 were found not to have syphilis. The nPCR results for...
Two major Treponema pallidum subtypes, 14 d/g and d/f, were identified in a population of 119 patients with syphilis Paris, France, characterized by high proportion men who have sex men. A new subtype named 11 q/j was characterized, reinfection case determined 1 patient having consecuitve infection at 19-month interval.
Background Treponema pallidum subsp. endemicum (TEN) is the causative agent of endemic syphilis (bejel). An unusual human TEN 11q/j isolate was obtained from a syphilis-like primary genital lesion patient that returned to France Pakistan. Methodology/Principal findings The characterized using nested PCR followed by Sanger sequencing and/or direct Illumina sequencing. Altogether, 44 chromosomal regions were analyzed. Overall, clustered with strains Bosnia A and Iraq B as expected previous...
Treponema pallidum subsp. pallidum, the causative agent of sexually transmitted syphilis, detected in clinical samples from France, was subjected to molecular typing using recently developed Multilocus Sequence Typing system. The (n = 133) used this study were collected 2010-2016 patients with diagnosed primary or secondary syphilis attending outpatient centers hospitals several locations France. Altogether, 18 different allelic profiles found among fully typed 112). There five variants...
Abstract Background. Syphilis remains a significant public health problem. We conducted prospective study to define more precisely the clinical and biological characteristics of patients with neurosyphilis (NS), we assessed diagnostic value nested polymerase chain reaction (PCR) testing for Treponema pallidum in cerebrospinal fluid (CSF) samples. Methods. From 2001 2013, included 40 (90% men; 45% infected human immunodeficiency virus) NS, defined as syphilis neurological and/or...
There is a lack of large studies appraising the effect human immunodeficiency virus (HIV) on course syphilis since advent highly active antiretroviral therapy (HAART). We aimed to appraise HIV clinical and serologic features at baseline during follow-up in post-HAART era. designed retrospective cohort study consecutive cases, diagnosed between 2000 2007, an academic venereal disease center. Data were collected using standardized medical forms. Patients treated according European guidelines....
Benzathine penicillin G (BPG) is the reference treatment for early syphilis, but shortages have recently been reported, highlighting a need validation of alternative treatments. The aim this study was to evaluate genomic resistance Treponema pallidum subspecies (TPA) macrolides and doxycycline in France. Swabs from genital, anal, oral cutaneous lesions were obtained 146 patients with syphilis They screened mutations conferring by nested PCR sequencing. Resistance detected 85% isolates, no...
BackgroundSyphilis in blood donors (BD) has increased many countries.AimWe aimed to describe trends syphilis seroposivity BD France, identify risk factors and assess if a non-treponemic test (NTT) could define having recovered from for more than 1 year.MethodsThe analysis covered the period 2007 2022 45,875,939 donations. Of 474 syphilis-positive 2022, 429 underwent additional investigations with an NTT. History of was obtained at post-donation interview or based on serology results repeat...
We evaluated the utility of commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum, herpes simplex virus 1 (HSV-1) and 2 (HSV-2), Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal ulcers context suspected syphilis. In total, 374 documented from patients with without syphilis presenting at several sexually transmitted infection (STI) centers France October 2010 to December 2016 were analyzed National Reference Center (CNR)...
Background: Childhood community-acquired pneumonia is a leading cause of childhood morbidity in low-income countries. The etiologic agents are usually Staphylococcus aureus, Streptococcus pneumoniae and Mycoplasma pneumoniae. M. was recognized as cofactor asthmatic disease. High asthma prevalence reported Madagascar. Our aim to clarify the infection this country its relationship with asthma. Methods: A prospective study conducted 351 children (from 2 16 years age) from January 2012 December...
Since 2000, the incidence of syphilis has risen in developed countries. An updated knowledge features is key for early diagnosis and treatment. Our objective was to appraise clinical epidemiological presentation Paris, France. A retrospective monocentric descriptive study 284 consecutive cases conducted a venereal disease centre (Paris, France), over period 2000-2007. Epidemiological, microbiological data were systematically collected, using standardized medical forms. Overall, 95% occurred...
Many assays are available on cerebrospinal fluid (CSF) for the diagnosis of neurosyphilis (NS) but there is no 'gold standard'.The aim this study was to evaluate different molecular and serological used in NS.We evaluated two PCR three techniques parallel CSF samples collected between 2019 2020 from patients suspected NS.The included 143 comprising 30 early NS, 7 late NS 106 without a NS. All with were symptomatic had either neurological (67.6%) or ophthalmological signs (54.1%). The qPCR...
Objectives We evaluated a real-time quantitative PCR (qPCR) for detection of the Treponema pallidum (TP) genome in clinical samples through simultaneous two genomic targets. Methods performed qPCR with TaqMan technology using TP genes, polA and tpp47 , as targets, an internal positive control. The assay was compared syphilis diagnosis based on combination examination, serological results inhouse nested (nPCR). Samples were analysed at National Reference Center STIs Cochin Hospital Paris....