A5090090556- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Antibiotics Pharmacokinetics and Efficacy
- Probiotics and Fermented Foods
- Microbial Metabolism and Applications
- Milk Quality and Mastitis in Dairy Cows
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
- Enterobacteriaceae and Cronobacter Research
- Infections and bacterial resistance
- Antibiotic Use and Resistance
- Streptococcal Infections and Treatments
- Irrigation Practices and Water Management
- Biochemical and Structural Characterization
- Listeria monocytogenes in Food Safety
- Clostridium difficile and Clostridium perfringens research
- Protein Hydrolysis and Bioactive Peptides
- Soil erosion and sediment transport
Tunis El Manar University
2015-2024
Tunis University
2015-2024
Faculté de médecine de Tunis
2016-2024
Centre de Recherches et des Technologies des Eaux
2023
Compagnie Africaine des Peintures
2022
Tunisia Private University
2020
Institute of Water Modelling
2018
University of Carthage
2017
Laboratoire National de Référence
2007
Staphylococcus aureus is recognized worldwide as one of the major agents dairy cow intra-mammary infections. This microorganism can express a wide spectrum pathogenic factors used to attach, colonize, invade and infect host. The present study evaluated 120 isolates from eight different countries that were genotyped by RS-PCR investigated for 26 virulence increase knowledge on circulating genetic lineages among population with mastitis. New genotypes observed South African strains while all...
Oxazolidinone resistance is a serious limitation in the treatment of MDR Enterococcus infections. Plasmid-mediated oxazolidinone has been strongly linked to animals where use phenicols might co-select both antibiotic families. Our goal was assess diversity genes conferring phenicol/oxazolidinone among diverse enterococci and characterize optrA genetic environment.Chloramphenicol-resistant isolates (>16 mg/L, n = 245) from different sources (hospitals/healthy humans/wastewaters/animals)...
Abstract Objectives The epidemiology of Enterococcus resistant to priority antibiotics including linezolid has mainly been investigated in developed countries and especially hospitals. We aimed evaluate the contribution different non-human reservoirs for burden MDR enterococci Tunisia, where scarce data are available. Methods Samples (n = 287) were collected from urban wastewater 57), retail meat 29; poultry/bovine/ovine), milk 89; bovine/ovine), farm animal faeces 80; poultry/bovine/ovine)...
Fifty-five Escherichia coli isolates were acquired from chicken and turkey meat obtained two slaughterhouses in Tunis. Eighty-nine percent, 80%, 78%, 67%, 45%, 27%, 7%, 4%, 2% of these showed resistance to tetracycline, trimethoprim/sulfamethoxazole, streptomycin, nalidixic acid, ampicillin, chloramphenicol, ciprofloxacin, colistine, gentamicin, respectively. No was detected cefotaxime, ceftazidime, or amikacin. bla(TEM) gene found 22 25 ampicillin-resistant isolates, 1 isolate harbored...
Abstract Objectives poxtA is the most recently described gene conferring acquired resistance to linezolid, a relevant antibiotic for treating enterococcal infections. We retrospectively screened in diverse enterococci and aimed characterize its genetic/genomic contexts. Methods was by PCR 812 from 458 samples (hospitals/healthy humans/wastewater/animals/retail food) obtained Portugal/Angola/Tunisia (1996–2019). Antimicrobial susceptibility testing performed 13 antibiotics (EUCAST/CLSI)....
Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is considered a major hindrance in poultry farming worldwide. This study aimed to characterize the genetic content and relatedness between multidrug-resistant E. isolates from broiler chickens died due colibacillosis three farms Tunisia. One hundred samples were collected chickens' fresh carcasses isolation identification performed. Then, antimicrobial susceptibility regarding antibiotics, ability produce β-lactamases minimum...
Background: Methicillin-resistant staphylococci (MRS) are an emerging global problem with serious public health concern.Aims: This study investigated the prevalence and antimicrobial susceptibility of commensal Staphylococcus species isolated from healthy clinical cats dogs.Methods: Nasal swab samples were collected animals processed using selective semi-selective mediums. Presumptive isolates subjected to biochemical testing analyzed Phoenix automated identification system. PCRs protocols...
Avian ESBL-producing Escherichia coli isolates have been increasingly reported worldwide. Animal to human dissemination, via food chain or direct contact, of these resistant bacteria has reported. In Tunisia, little is known about avian ESBL- producing E. and further studies are needed. Seventeen from poultry faeces two farms (Farm 1 farm 2) in the North Tunisia used this study. Eleven (from 1) same resistance profile nalidixic acid, sulfonamides, streptomycin, tetracycline norfloxacine...
Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses industry worldwide. In addition, milk and milk-products contaminated by Staph. can cause harmful human diseases. The aim this study was to characterize strains isolated from farms Tunisia. Bulk tank (n = 32) individual cow 130) samples were collected during the period 2013-2014. Forty-three isolates recovered typed spa typing, 16S-23S rRNA intergenic spacer (RS-PCR) multiplex PCRs for 22...
Increasing numbers of linezolid-resistant Enterococcus carrying optrA are being reported across different niches worldwide. We aimed to characterize the first optrA-carrying faecalis obtained from food-producing animals and retail meat samples in Tunisia.Seven E. chicken faeces (n=3, August 2017) (n=4, Tunisia were analysed. Antimicrobial susceptibility was determined by disc diffusion, broth microdilution Etest against 13 antibiotics, linezolid tedizolid, respectively (EUCAST/CLSI)....
Acinetobacter baumannii has emerged as a main nosocomial pathogen exhibiting high rates of resistance to clinically relevant antibiotics. Six pandrug-resistant A. (PDR-A. baumannii) were recovered from three patients in Tunisian Intensive Care Unit (ICU) between 10th and 16th May 2018 resulting one fatal case raising the possibility an outbreak. On 18th environmental screening ICU surfaces was carried out. 22nd fourth patient infected with PDR-A. died. A second investigation out for isolated...
To investigate the safety and technological traits of previously isolated bacteriocinogenic enterococci strains for potential use as starter/adjunct cultures in foods. Fifty‐five from different origins Tunisia were screened safety. Twenty‐two did not harbour genes coding virulence traits, susceptible to relevant antibiotics such vancomycin, tested negative haemolysis, histamine production, gelatinase activity DNase activity. These further assessed some properties, demonstrating low...
Escherichia coli (E. coli) is one of the main etiological agents responsible for bovine mastitis (BM), neonatal calf diarrhea (NCD), and avian colibacillosis (AC). This study aimed to assess resistance virulence genes content, biofilm-forming ability, phylogenetic groups, genetic relatedness in E. isolates recovered from clinical cases BM, NCD, AC.A total 120 samples including milk (n = 70) feces 50) cows with BM calves respectively, were collected different farms Northern Tunisia. Bacterial...
Antimicrobial-resistant Escherichia coli isolates have emerged in various ecologic compartments and evolved to spread globally. We sought (1.) investigate the occurrence of ESBL-producing E. (ESBL-Ec) feces from free-range chickens a rural region (2.) characterize genetic background antimicrobial resistance relatedness collected isolates. Ninety-five swabs associated with two households (House 1/House 2) northern Tunisia were collected. Samples screened recover ESBL-Ec, characterized for...
The objective was to characterize Staphylococcus aureus isolated from two wastewater treatment plants (WWTPs) located in Tunis City (Tunisia), during the period 2014–2015. Genetic lineages, antibiotic resistance mechanisms and virulence factors were determined for recovered isolates. S. isolates 12 of 62 samples tested (19.35%), one isolate/sample characterized, all them being methicillin-susceptible (MSSA). Six spa types (t587, t674, t224, t127, t701 t1534) found among isolates, spa-t587,...
This study focused on 77 isolates of Salmonella enterica serotype Enteritidis collected during 2009 to 2013 from healthy and sick chickens environmental farm samples in Tunisia. Resistance 14 antimicrobials the encoding genes were analyzed. 66, 26, 6.5, 3.9 1.3% pan-susceptible or showed resistance nalidixic acid (Asp87 Tyr Asp87 Asn substitutions GyrA), ampicillin (blaTEM-1-like blaSHV), sulfonamides (sul1and sul3) streptomycin (strB), respectively. A single isolate with intermediate...