Abstract Objectives poxtA is the most recently described gene conferring acquired resistance to linezolid, a relevant antibiotic for treating enterococcal infections. We retrospectively screened in diverse enterococci and aimed characterize its genetic/genomic contexts. Methods was by PCR 812 from 458 samples (hospitals/healthy humans/wastewater/animals/retail food) obtained Portugal/Angola/Tunisia (1996–2019). Antimicrobial susceptibility testing performed 13 antibiotics (EUCAST/CLSI)....

Polysulfated (oligo)flavonoids were synthesized and assayed for their in vitro vivo anticoagulant activities. The approach was based on molecular hybridization of two classes anticoagulants, sulfated polysaccharides flavonoids. synthesis optimized using microwave-assisted sulfation with triethylamine-sulfur trioxide. obtained polysulfated flavonosides highly effective increasing clotting times able to completely block the process, contrast corresponding aglycones. thromboelastography proved...

A multipathway strategy was used to evaluate the in vitro and vivo antithrombotic effects of a new synthetic family sulfated small molecules. Polysulfated xanthonosides showed highly effective anticoagulation vitro, both plasma (clotting times) whole human blood (thromboelastography), as well (ip administration, mice). Physicochemical properties were assessed for mangiferin heptasulfate (7), which high solubility stability water no putative hepatotoxicity vivo. Mangiferin (7) found be direct...

Increasing numbers of linezolid-resistant Enterococcus carrying optrA are being reported across different niches worldwide. We aimed to characterize the first optrA-carrying faecalis obtained from food-producing animals and retail meat samples in Tunisia.Seven E. chicken faeces (n=3, August 2017) (n=4, Tunisia were analysed. Antimicrobial susceptibility was determined by disc diffusion, broth microdilution Etest against 13 antibiotics, linezolid tedizolid, respectively (EUCAST/CLSI)....

Poultry meat has been a vehicle of antibiotic resistant bacteria and genes. Yet, the diversity selective pressures associated with their maintenance in poultry-production chain remains poorly explored. We evaluated susceptibility Enterococcus spp. from chicken collected 20 years apart to antibiotics, metals, acidic pH peracetic acid-PAA. Contemporary chicken-meat samples (n = 53 batches, each including pool neck skin 10 single carcasses) were slaughterhouse facility using PAA as disinfectant...

Enterococcus spp. are opportunistic human pathogens colonizing the gut and a significant reservoir for continuous adaptation of hospital clones. However, studies on features enterococci species co-colonizing healthy individuals scarce. We investigated prevalence, antibiotic resistance, bacteriocin profiles in fecal samples from adults Portugal using culture-based methods, WGS, inhibition assays. Results were compared with data 2001 study same region. (n = 315; 24% MDR) recovered all...

Multidrug-resistant (MDR) Enterococcus faecium (Efm) infections continue to increase worldwide, although epidemiological studies remain scarce in lower middle-income countries. We aimed explore which strains circulate E. causing human Tunisian healthcare institutions order compare them with from non-human sources of the same country and finally position within global epidemiology by genomic analysis. Antibiotic susceptibility testing was performed transfer vancomycin-vanA ampicillin-pbp5...

Abstract We describe enterococci in raw-frozen dog food commercialized Europe as a source of genes encoding resistance to the antibiotic drug linezolid and strains plasmids enriched antibiotic-resistance virulence hospitalized patients. Whole-genome sequencing was fundamental linking isolates from human cases across Europe.

Chlorhexidine (CHX) is a broad-spectrum antiseptic widely used in community and clinical contexts for many years that has recently acquired higher relevance nosocomial infection control worldwide. Despite this, CHX tolerance among Enterococcus faecium bacteria, representing one of the leading agents causing infections, been poorly understood. This study provides new phenotypic molecular data better identification CHX-tolerant E. subpopulations contexts. The chlorhexidine MIC (MICCHX)...

Abstract We assessed the impact of oligofructose (OFS) and dextrin (DEX) as diet supplements on hepatic redox state. Rats were fed either a 10% OFS or DEX supplemented for 9 wk. In group, levels protein carbonylation decreased by 63%. Total glutathione reduced (GSH) contents in groups around 20%. supplementation significantly oxidized (GSSG) resulting 33% increase GSH/GSSG ratio. The activity antioxidant enzymes was not changed supplementation. caused decrease serum triglycerides (36%),...

Acid stress poses a common challenge for bacteria in diverse environments by the presence of inorganic (e.g., mammals' stomach) or organic acids feed additives; acid-based disinfectants). Limited knowledge exists regarding acid-tolerant strains specific serotypes, clonal lineages, sources human/animal pathogens: namely, non-typhoidal Salmonella enterica (NTS) and Enterococcus faecium (Efm). This study evaluated acidic pH (Mueller-Hinton acidified with HCl) peracetic acid (PAA) susceptibility...

O presente estudo teve como objetivo analisar o poder calorifico e a densidade basica da madeira das especies Luetzelburgia auriculata Allemao (Ducke) , Combretum leprosum Mart., Jatropha mutabilis (Pohl) Baill Poincianella bracteosa (Tul.) L. P. Queiroz Mimosa tenuiflora (Willd.) Poir. Croton argirophylloides Mull. Arg., em uma area de Caatinga, Coremas-PB Sclerolobium paniculatum Vogel aureum Benth. Plathymenia reticulata Qualea grandiflora Mart. Aspidosperma macrocarpum Indigofera...

Acid stress poses a common challenge for bacteria in diverse environments by the presence of inorganic (e.g., mammals’ stomach) or organic acids feed additives; acid-based disinfectants). Limited knowledge exists regarding acid-tolerant strains specific serotypes, clonal lineages, sources human/animal pathogens, namely non-typhoidal Salmonella enterica (NTS) and Enterococcus faecium (Efm). This study evaluated acidic pH peracetic acid (PAA) susceptibility Efm (n=72) NTS (n=60) from...

Sulfated phenolic polymers have extensively been investigated as anticoagulant agents in view of their higher bioavailability and resistance to degradation compared heparins, allowing for increased half-lives. In this frame, we report herein the preparation sulfated derivatives tyrosol, one most representative constituents extra virgin olive oil, by different approaches. Mild sulfation OligoTyr, a mixture tyrosol oligomers, that has reported possess antioxidant properties osteogenic...

Genetic redundancy in bacteria plays a crucial role enhancing adaptability and accelerating evolution response to selective pressures, particularly those associated with rapid environmental changes. Aminopenicillins like ampicillin are important therapeutic options for Enterococcus infections both humans animals, resistance mostly pbp5 gene mutations or overexpression. While the occurrence of redundant genes has been occasionally reported, advantages host have not explored detail. During...

The use of antibiotics in animal production is linked to the emergence and spread antibiotic-resistant bacteria, a threat animal, environmental human health. Copper (Cu) an essential element poultry diets alternative antibiotics, supplementing inorganic or organic trace mineral feeds (ITMF/OTMF). However, its contribution select multidrug-resistant (MDR) Cu tolerant Enterococcus, bacteria with human-animal-environment-food interface, remains uncertain. We evaluated whether feeding chickens...

Introduction: Forensic Microbiology is an emerging discipline in clinical diagnostics and public health that can be applied, for example, to clarify the origin of a hospital-acquired infection [1].Enterococcus faecium (Efm) colonizes human gut but also one leading hospital pathogen worldwide.Last year, taxonomic restructuration proposed Efm inhabiting may actually correspond Enterococcus lactis species, while those causing infections are truly [2].Objectives: Our main goal was assess current...