A5085673878- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Metalloenzymes and iron-sulfur proteins
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Biofuel production and bioconversion
- Plant Stress Responses and Tolerance
- Drug Transport and Resistance Mechanisms
- Microbial Fuel Cells and Bioremediation
- ATP Synthase and ATPases Research
- Plant Genetic and Mutation Studies
- Plant Parasitism and Resistance
- Nitrogen and Sulfur Effects on Brassica
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Phytase and its Applications
- Biocrusts and Microbial Ecology
- RNA modifications and cancer
- Marine and coastal ecosystems
- Plant tissue culture and regeneration
- Algal biology and biofuel production
- Folate and B Vitamins Research
University of Milan
2020-2022
Max Planck Institute of Molecular Plant Physiology
2018-2021
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used species such as
The composition of the thylakoid proton motive force (pmf) is regulated by ion transport. Passive channels in membrane dissipate potential (Ɗψ) component to allow for a higher fraction pmf stored as concentration gradient (ƊpH). K+/H+ antiport across via K+ EXCHANGE ANTIPORTER3 (KEA3) instead reduces ƊpH pmf. Thereby, KEA3 decreases nonphotochemical quenching (NPQ), thus allowing light use efficiency, which particularly important during transitions from high low light. Here, we show that...
Protein phosphorylation is a well-established post-translational mechanism that regulates protein functions and metabolic pathways. It known several plant mitochondrial proteins are phosphorylated in reversible manner. However, the identities of kinases/phosphatases involved this their roles regulation tricarboxylic acid (TCA) cycle remain unclear. In study, we isolated characterized plants lacking two mitochondrially targeted phosphatases (Sal2 PP2c63) along with pyruvate dehydrogenase...
Summary The NADH:ubiquinone oxidoreductase (respiratory complex I) is the main entry point for electrons into Escherichia coli aerobic respiratory chain. With its sophisticated setup of 13 different subunits and 10 cofactors, it anticipated that various chaperones are needed proper maturation. However, very little known about assembly E. I, especially concerning incorporation iron‐sulfur clusters. To identify cluster carrier proteins possibly involved in process, we generated knockout...
Abstract The majority of cellular processes are carried out by protein complexes. Various size fractionation methods have previously been combined with mass spectrometry to identify However, most these approaches lack the quantitative information which is required understand how changes complex abundance and composition affect metabolic fluxes. In this paper we present a proof concept approach quantitatively study complexome in model plant Arabidopsis thaliana at end day (ED) night (EN). We...
Abstract Enzyme‐enzyme interactions can be discovered by affinity purification mass spectrometry (AP‐MS) under in vivo conditions. Tagged enzymes either transiently transformed into plant leaves or stably cells prior to AP‐MS. The success of AP‐MS depends on the levels and stability bait protein, protein‐protein interactions, efficiency trypsin digestion recovery tryptic peptides for MS analysis. Unlike in‐gel‐digestion AP‐MS, which gel is cut pieces several independent digestions, we uses a...
Abstract Transcriptional reprogramming plays a key role in drought stress responses, preceding the onset of morphological and physiological acclimation. The best-characterized signal regulating gene expression response to is phytohormone abscisic acid (ABA). ABA-regulated expression, biosynthesis signaling are highly organized diurnal cycle, so that traits occur at appropriate time day. mechanisms underpin such diel oscillations ABA signals poorly characterized. Here we uncover GIGANTEA (GI)...
Molybdenum cofactor (Moco) biosynthesis is a complex process that involves the coordinated function of several proteins. In recent years it has become obvious availability iron plays an important role in Moco. First, MoaA protein binds two [4Fe-4S] clusters per monomer. Second, expression moaABCDE and moeAB operons regulated by FNR, which senses oxygen via functional cluster. Finally, conversion cyclic pyranopterin monophosphate to molybdopterin requires l-cysteine desulfurase IscS, shared...
The mitochondrial ATP-binding cassette (ABC) transporters ABCB7 in humans, Atm1 yeast and ATM3 plants, are highly conserved their overall architecture particularly glutathione binding pocket located within the transmembrane spanning domains. These have attracted interest last two decades based on proposed role connecting iron-sulfur (Fe-S) cluster assembly with its cytosolic Fe-S (CIA) counterpart. So far, specific compound that is transported across membrane remains unknown. In this report...