A comprehensive investigation was carried out to determine the changes that occurred in water-stressed cucumber (Cucumis sativus L.) response melatonin treatment. We examined potential roles of during seed germination and root generation measured its effect on reactive oxygen species (ROS) levels, antioxidant enzyme activities, photosynthesis. Melatonin alleviated polyethylene glycol induced inhibition germination, with 100 μm melatonin-treated seeds showing greatest rate. stimulated...

Although previous studies have found that melatonin can promote seed germination, the mechanisms involved in perceiving and signaling remain poorly understood. In this study, it was synthesized during cucumber germination with a peak levels occurring 14 hr into germination. This is indicative of correlation between synthesis Meanwhile, seeds pretreated exogenous (1 μM) showed enhanced rates under 150 mM NaCl stress compared to water-pretreated salinity stress. There are two apparent by which...

In this study, the effect of melatonin on postharvest ripening and quality improvement tomato fruit was carried out. The tomatoes were immersed in exogenous for 2h, then related physiological indicators expression genes during post-harvest life evaluated. Compared with control check (CK), 50 µM treatment significantly increased lycopene levels by 5.8-fold. Meanwhile, key involved colour development, including phytoene synthase1 (PSY1) carotenoid isomerase (CRTISO), showed a 2-fold increase...

Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused its physiological influence growth development, biosynthesis. Much less attention has been drawn to affect genome-wide gene expression. To comprehensively investigate the role(s) of at genomics level, we utilized mRNA-seq technology analyze Arabidopsis subjected 16-hour 100 pM (low) 1 mM (high) treatment. The expression profiles were analyzed identify...

Abstract Cucumber is a model cucurbitaceous plant with known genome sequence which important for studying molecular mechanisms of root development. In this study, RNA sequencing was employed to explore the mechanism melatonin‐induced lateral formation in cucumber under salt stress. Three groups seeds were examined, that is, primed without melatonin ( CK ), solution containing 10 or 500 μmol/L (M10 and M500, respectively). These then germinated NaCl solution. The ‐seq analysis generated...

In this work, we found, that exogenous melatonin pretreatment improved anthocyanin accumulation (1- to 2-fold) in cabbage. To verify the relationship with and anthocyanin, an Arabidopsis mutant, snat, which expresses a defective form of biosynthesis enzyme SNAT (Serotonin N-acetyl transferase), was employed. Under cold conditions, foliage wild-type exhibited deeper red color than snat mutant. This finding further proved, treatment able affect accumulation. gain better understanding how...

Telomerase is an essential enzyme that maintains telomeres on eukaryotic chromosomes. In mammals, telomerase required for the lifelong proliferative capacity of normal regenerative and reproductive tissues sustained growth in a dedifferentiated state. Although importance was first elucidated plants 60 years ago, little known about role plant development. Here we report cloning characterization Arabidopsis reverse transcriptase (TERT) gene, AtTERT. AtTERT predicted to encode highly basic...

Abstract The Arabidopsis (Arabidopsis thaliana) gene BT2 encodes a 41-kD protein that possesses an amino-terminal BTB domain, central TAZ and carboxyl-terminal calmodulin-binding domain. We previously demonstrated could activate telomerase expression in mature leaves. Here, we report its distinct role mediating diverse hormone, stress, metabolic responses. serendipitously discovered steady-state of mRNA was regulated diurnally controlled by the circadian clock, with maximum dark. This...

Melatonin plays an important role in stress tolerance plants. In this study, exogenous melatonin significantly alleviated the dwarf phenotype and inhibited decrease of plant fresh weight induced by excess copper (Cu2+). Our results indicated that Cu2+ toxicity improving sequestration, carbon metabolism ROS (reactive oxygen species) scavenging, rather than influencing uptake under conditions. Transcriptome analysis showed broadly altered gene expression stress. increased levels glutathione...

The NAC transcription factors play vital roles in responding to drought stress plants; however, the molecular mechanisms remain largely unknown cucumber. Suppression of CsATAF1 via RNA interference (RNAi) weakened tolerance cucumber due a higher water loss rate leaves, level hydrogen peroxide (H2O2) and superoxide radicals (O2·-), increased malondialdehyde (MDA) content, lower Fv/Fm ratios antioxidant enzyme activity. analysis root length stomatal apertures showed that CsATAF1-RNAi plants...

Melatonin (N-acetyl-5-methoxytryptamine) plays important roles in plant developmental growth, especially root architecture. The similarity both chemical structure and biosynthetic pathway suggests a potential linkage between melatonin auxin signaling. However the molecular mechanism regulating this melatonin-mediated architecture changes is not yet elucidated. In present study, we re-analyzed previously conducted transcriptome data identified 16 auxin-related genes whose expression patterns...

SUMMARY Cucumber ( Cucumis sativus ) originated in tropical areas and is very sensitive to low temperatures. Cold acclimation a universal strategy that improves plant resistance cold stress. In this study, we report heat shock induces cucumber seedlings, via process involving the heat‐shock transcription factor HSFA1d. CsHSFA1d expression was improved by both treatment. Moreover, transcripts accumulated more under treatment after pre‐treatment than with either or alone. After exposure cold,...

Abstract Jasmonic acid (JA) is a key regulator of plant defense responses. Although the transcription factor MYC2, master JA signaling pathway, orchestrates hierarchical transcriptional cascade that regulates responses, only few regulators involved in this have been described. Here, we identified basic helix‐loop‐helix (bHLH) gene tomato ( Solanum lycopersicum ), METHYL JASMONATE MeJA ) ‐INDUCED GENE SlJIG expression which was strongly induced by treatment. Genetic and molecular biology...

Soybean seed weight is not only a yield component, but also critical trait for various soybean food products such as sprouts, edamame, soy nuts, natto and miso. Linkage analysis genome-wide association study (GWAS) are two complementary powerful tools to connect phenotypic differences the underlying contributing loci. based on progeny derived from parents, given sufficient sample size biological replication, it usually has high statistical power map alleles with relatively small effect...

Abstract The function of aquaporin (AQP) protein in transporting water is crucial for plants to survive drought stress. With 47 homologues tomato ( Solanum lycopersicum ) were reported, but the individual and integrated functions aquaporins involved response remains unclear. Here, three plasma membrane intrinsic genes, SlPIP2;1, SlPIP2;7 SlPIP2;5, identified as candidate genes because highly expressed roots. Assay on expression Xenopus oocytes demonstrated that SlPIP2s displayed channel...

Abstract Improving plant water‐use efficiency (WUE) is important to survival and crop yield in the context of water limitation. In this study, SlTLFP8 (Tubby‐like F‐box protein 8) was identified as an osmotic‐induced gene tomato. Transgenic tomato with up‐regulated expression showed enhanced water‐deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive deficit. overexpression significantly WUE suppressing transpiration under both water‐sufficient...

Telomerase, an enzyme essential for the synthesis and maintenance of telomeric DNA long-term stability genome, is developmentally regulated in plants. Telomerase activity abundant reproductive organs but low or undetectable vegetative organs. Treatment with exogenous auxin, however, overrides this developmental control induces telomerase mature leaves. The Arabidopsis thaliana transcription factor TELOMERASE ACTIVATOR1 (TAC1) potentiates some responses to including induction Here, we report...

Sweet sorghum (Sorghum bicolor [L.] Moench) accumulates fermentable sugars in the stem and is increasingly being studied as a potential source of feedstock for bioethanol production. The objective this study was to evaluate biomass grain yield five sweet cultivars (Dale, M81E, Sugar Drip, Della Keller) determine quality extractable juice grain. Randomized complete block experiments were performed summer 2009, 2010, 2011. Leaf dry weight varied with year cultivar averaged 6177 kg·ha-1. Fresh...

NACs are one of the largest transcription factor families in plants and involved response to abiotic stress. BoNAC019, a homologue AtNAC019, was isolated from cabbage (Brassica oleracea). BoNAC019 localized nucleus functioned as transcriptional activator. The expression induced by dehydration, salt, abscisic acid (ABA), H

The effects of water stress on leaf surface morphology (stomatal density, size, and trichome density both adaxial abaxial surfaces) ultrastructure (chloroplasts, mitochondria, cell nuclei) eggplant (Solanum melongena L.) were investigated in this study. Higher stomata densities observed compared with the surface. Compared well watered (WW) plants, increased by 20.39% 26.23% under water-stress condition, respectively. number chloroplasts per profile was lesser, became round a shape more...

The small genome of sorghum (Sorghum bicolor L. Moench.) provides an important template for study closely related large-genome crops such as maize (Zea mays) and sugarcane (Saccharum spp.), is a logical complement to distantly rice (Oryza sativa) "grass model." Using high-density RFLP map framework, robust physical being assembled by integrating hybridization fingerprint data with comparative from taxa using new methods resolve genomic duplications into locus-specific groups. By taking...