A5061253161- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Glycosylation and Glycoproteins Research
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Signaling Pathways in Disease
- Cancer Research and Treatments
- Inflammatory Bowel Disease
- Glioma Diagnosis and Treatment
- Calcium signaling and nucleotide metabolism
- Adipose Tissue and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antifungal resistance and susceptibility
- Pharmacological Effects of Natural Compounds
- Galectins and Cancer Biology
- Multiple Myeloma Research and Treatments
- Toxin Mechanisms and Immunotoxins
- Drug-Induced Adverse Reactions
Istituto di Farmacologia Traslazionale
2015-2025
National Research Council
2014-2025
Saint Camillus International University of Health and Medical Sciences
2025
National Academies of Sciences, Engineering, and Medicine
2013-2024
University of Basel
2008-2014
University of Rome Tor Vergata
1991-2014
Confederazione Nazionale dell'Artigianato e Della Piccola e Media Impresa
2014
Ospedale San Giovanni Bellinzona
2014
Ospedale regionale di Lugano
2014
Kantonsschule Olten
2014
Recent evidence suggests that natural killer (NK) cells are typically defective in infiltrating solid tumors, with the exception of gastrointestinal stromal tumors (GIST). Interestingly, however, infrequently NK do not appear to have a direct effect on tumor progression. Here, prompted by recent cell and T crosstalk may trigger, or enhance, antigen-specific immune responses, we tested clinical significance this reciprocal signaling. To end, tissue microarray constructed 1410 colorectal...
The goal of this study was to determine the frequency HLA class II antigen expression in colorectal carcinoma (CRC) tumors, its association with clinical course disease, and underlying mechanism(s). Two tissue microarrays constructed 220 778 CRC tumors were stained HLA-DR, DQ, DP antigen-specific monoclonal antibody LGII-612.14, using immunoperoxidase staining technique. immunohistochemical results correlated disease. functional role antigens expressed on cells analyzed by investigating...
Enhanced oxidative stress contributes to the pathogenesis of diabetes and its complications. Peroxiredoxin 6 (PRDX6) is a key regulator cellular redox balance, with peculiar ability neutralize peroxides, peroxynitrite, phospholipid hydroperoxides. In current study, we aimed define role PRDX6 in pathophysiology type 2 (T2D) using knockout (-/-) mice. Glucose insulin responses were evaluated respectively by intraperitoneal glucose tolerance tests. Peripheral sensitivity was analyzed...
Background Colorectal cancer (CRC) infiltration by adaptive immune system cells correlates with favorable prognosis. The role of the innate is still debated. Here we addressed prognostic impact CRC neutrophil granulocytes (NG). Methods A TMA including healthy mucosa and clinically annotated specimens (n = 1491) was stained MPO CD15 specific antibodies. MPO+ CD15+ positive were counted three independent observers. Phenotypic profiles infiltrating validated flow cytometry on cell suspensions...
The prognostic significance of macrophage and natural killer (NK) cell infiltration in colorectal carcinoma (CRC) microenvironment is unclear. We investigated the CRC innate inflammatory infiltrate over 1,600 using two independent tissue microarrays immunohistochemistry. Survival time was assessed Kaplan-Meier method Cox proportional hazards regression analysis a multivariable setting. Spearman's rank correlation tested association between lymphocyte infiltration. Basel study included 1,400...
Colorectal cancer infiltration by CD16(+) myeloid cells correlates with improved prognosis. We addressed mechanistic clues and gene protein expression of cytokines potentially associated macrophage polarization.GM-CSF or M-CSF-stimulated peripheral blood CD14(+) from healthy donors were cocultured colorectal cells. Tumor cell proliferation was assessed (3)H-thymidine incorporation. Expression cytokine genes in autologous mucosa tested quantitative, real-time PCR. A tumor microarray (TMA)...
T cells are primary targets in numerous gene therapy protocols. However, the use of subgroup C adenovirus serotype 2 or 5 (Ad2 Ad5) as a vector to transduce is limited by its poor transduction efficiency for these cells. In this report we show that T-cell results from lacking both Ad2-Ad5 receptor, used attachment, and secondary Ad which mediates entry most serotypes. These deficiencies were overcome using bispecific antibody (bsAb) with specificities human CD3 FLAG epitope genetically...
Abstract The primary granule proteins (PGP) of myeloid cells are a source multiple antigens with immunotherapeutic potential for leukemias. Therefore, we developed method to induce T-cell responses PGP protein sequences. We found that gene-transfected antigen-presenting efficiently expand functionally competent PGP-specific CD4 and CD8 T cells. system was optimized using autologous CD40-activated B (CD40-B) transfected cytomegalovirus pp65-encoding expression vector. To generate...
Infection with BK virus (BKV) induces both humoral and cellular immunity, but the viral antigens of T-antigen (T-ag) stimulating T-cell responses are largely unknown. To identify BKV-specific T cells in healthy individuals, peripheral blood lymphocytes were cultured autologous dendritic (DCs) loaded BKV lysate screened for intracellular gamma interferon production after stimulation an overlapping 15mer peptide library T-ag. Among many immunogenic peptides identified, four T-ag identified as...