A5033802441- CAR-T cell therapy research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Glioma Diagnosis and Treatment
- Nanowire Synthesis and Applications
- Endoplasmic Reticulum Stress and Disease
- Aesthetic Perception and Analysis
- Apelin-related biomedical research
- Hematopoietic Stem Cell Transplantation
- Chemokine receptors and signaling
- Metabolomics and Mass Spectrometry Studies
- Redox biology and oxidative stress
- Hormonal Regulation and Hypertension
- Multisensory perception and integration
- Pharmacological Effects of Natural Compounds
- Advancements in Semiconductor Devices and Circuit Design
- Extracellular vesicles in disease
- RNA regulation and disease
- RNA modifications and cancer
- Aldose Reductase and Taurine
- MicroRNA in disease regulation
Istituto di Farmacologia Traslazionale
2014-2025
National Research Council
2014-2025
National Academies of Sciences, Engineering, and Medicine
2024
University of Rome Tor Vergata
2014-2015
Universidad de La Laguna
2014
Confederazione Nazionale dell'Artigianato e Della Piccola e Media Impresa
2014
Recent evidence suggests that natural killer (NK) cells are typically defective in infiltrating solid tumors, with the exception of gastrointestinal stromal tumors (GIST). Interestingly, however, infrequently NK do not appear to have a direct effect on tumor progression. Here, prompted by recent cell and T crosstalk may trigger, or enhance, antigen-specific immune responses, we tested clinical significance this reciprocal signaling. To end, tissue microarray constructed 1410 colorectal...
The goal of this study was to determine the frequency HLA class II antigen expression in colorectal carcinoma (CRC) tumors, its association with clinical course disease, and underlying mechanism(s). Two tissue microarrays constructed 220 778 CRC tumors were stained HLA-DR, DQ, DP antigen-specific monoclonal antibody LGII-612.14, using immunoperoxidase staining technique. immunohistochemical results correlated disease. functional role antigens expressed on cells analyzed by investigating...
Enhanced oxidative stress contributes to the pathogenesis of diabetes and its complications. Peroxiredoxin 6 (PRDX6) is a key regulator cellular redox balance, with peculiar ability neutralize peroxides, peroxynitrite, phospholipid hydroperoxides. In current study, we aimed define role PRDX6 in pathophysiology type 2 (T2D) using knockout (-/-) mice. Glucose insulin responses were evaluated respectively by intraperitoneal glucose tolerance tests. Peripheral sensitivity was analyzed...
Cetuximab and panitumumab bind the human epidermal growth factor receptor (EGFR). Although chimeric cetuximab (IgG1) triggers antibody-dependent-cellular-cytotoxicity (ADCC) of EGFR positive target cells, (a IgG2) does not. The inability to trigger ADCC reflects poor binding affinity IgG2 Fc for FcγRIII (CD16) on natural killer (NK) cells. However, both IgG1 FcγRII (CD32A) a similar extent. Our study compares ability T engineered with novel low-affinity CD32A131R -chimeric (CR), those...
Recent studies have shown that CD32/CD8a/CD28/CD3ζ chimeric receptor cells directly kill breast cancer cells, suggesting the existence of cell surface myeloid FcγR alternative ligands (ALs). Here, we investigated metabolism, ALs, cytotoxicity, and immunoregulatory functions CD64/CD28/CD3ζ in colorectal (CRC) squamous carcinoma head neck. The -SFG retroviral vector was used to produce viruses for T-cell transduction. expansion differentiation were monitored via flow cytometry. Gene expression...
KRAS mutations hinder therapeutic efficacy of epidermal growth factor receptor (EGFR)-specific monoclonal antibodies cetuximab and panitumumab-based immunotherapy EGFR+ cancers. Although inhibits KRAS-mutated cancer cell in vitro by natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC), colorectal carcinoma (CRC) cells escape NK immunosurveillance vivo. To overcome this limitation, we used panitumumab to redirect Fcγ chimeric (CR) T against HCT116 cells. We...
Increasing evidence suggests that HLA-DRB1 alleles reduce or increase the risk of developing ulcerative colitis-associated colorectal carcinoma (CRC) tumors. However, role locus on susceptibility to develop CRC tumor, in absence a history inflammatory bowel diseases (IBDs), is unclear. The aim our study was determine whether are associated with IBD-independent tumor. allele polymorphisms were identified by sequence-based typing method 53 patients and 57 sex- age-matched healthy Caucasian...
// Roberto Arriga 1 , Sara Caratelli 2 Andrea Coppola Giulio Cesare Spagnoli 3 Adriano Venditti 4 Sergio Amadori Giulia Lanzilli Davide Lauro Patrizia Palomba Tommaso Sconocchia Maria Ilaria Del Principe Luca Maurillo Francesco Buccisano Barbara Capuani Soldano Ferrone 5 Giuseppe Department of Systems Medicine, University Rome "Tor Vergata", Rome, Italy Laboratory Tumor Immunology and Immunotherapy, Institute Translational Pharmacology, Biomedicine, National Research Council (CNR), for...
Liver has a principal role in glucose regulation and lipids homeostasis. It is under complex control by substrates such as hormones, nutrients, neuronal impulses. Insulin promotes glycogen synthesis, lipogenesis, lipoprotein synthesis inhibits gluconeogenesis, glycogenolysis, VLDL secretion modifying the expression enzymatic activity of specific molecules. To understand pathophysiological mechanisms leading to metabolic liver disease, we analyzed protein patterns expressed mouse model...
The FcγRII (CD32) ligands are IgFc fragments and pentraxins. existence of additional is unknown. We engineered T cells with human chimeric receptors resulting from the fusion between CD32 extracellular portion transmembrane CD8α linked to CD28/ζ chain intracellular moiety (CD32-CR). Transduced recognized three breast cancer (BC) one colon cell line among 15 tested in absence targeting antibodies. Sensitive BC conjugation CD32-CR induced polarization down-regulation, CD107a release, mutual...
<title>Abstract</title> Background Recent studies have shown that CD32/CD8a/CD28/CD3ζ chimeric receptor cells directly kill breast cancer cells, suggesting the existence of cell surface myeloid FcγR alternative ligands (ALs). Here, we investigated metabolism, ALs, cytotoxicity, and immunoregulatory functions CD64/CD28/CD3ζ in colorectal (CRC) squamous carcinoma head neck. Methods The -SFG retroviral vector was used to produce viruses for T-cell transduction. expansion differentiation were...
Color is a powerful visual stimulus to be considered in the design of narrative experience, because it closely related emotions and can guide users nonlinear subjectively. So, this paper discusses presents study on evaluation user experience non-linear audiovisual narrative, which includes techniques such as structured observation, cardsorting, questionnaires eyetracking. The results show that color helps navigating structures.
Glioblastoma multiforme (GBM) is the deadliest human brain tumor with a median survival following diagnosis of 14-16 months.Innovative therapeutic approaches are urgently needed.Cancer stem cells (CSC) from GBM resist current chemo-and radiotherapies and can generate recurrent aggressive tumors.To envisage innovative potential clinical use, we engineered T Fcγ-chimeric receptors (CRs) to elicit antibody-dependent cellular cytotoxicity (ADCC) in presence mAbs specific for associated an-
Meeting abstracts Natural killer (NK) cells are highly effective in controlling tumour growth, mice, but have no significant effect humans. The reason(s) of this phenomenon is(are) unclear. effects cancer on NK during target-effector cell conjugation was investigated
Abstract Allogeneic leukemia and cancer epithelial (CE) cells have been recently shown to induce,in vitro, NK cell abnormalities (NKCA). The latter include depletion apoptosis as well natural cytotoxic receptor (NCR) CD16 down-regulation. potential negative impact of CE induced NKCAs on the in vivo anti-tumor activity limited information about strategies protect from prompted us characterize develop counteract them. NKCA induction by is influenced neither interleukin-2 (IL-2) treatment nor...
ABSTRACT Cetuximab and panitumumab bind the human epidermal growth factor receptor (EGFR). While chimeric cetuximab (IgG1) triggers antibody-dependent-cellular-cytotoxicity (ADCC) of EGFR positive target cells, (a IgG2) does not. The inability to trigger ADCC reflects a poor binding affinity IgG2 Fc for FcγRIII (CD16) on NK cells. However, both IgG1 FcγRII (CD32) similar extent. Here, we have compared ability T engineered with novel low-affinity CD32 131R -chimeric (CR), those CD16 158F –CR...
ABSTRACT KRAS mutation hinders the therapeutic efficacy of epidermal-growth-factor-receptor (EGFR) mAb (cetuximab and panitumumab)-based immunotherapy EGFR+ cancers. Although, cetuximab controls KRAS-mutated cancer cell growth in vitro utilizing a NK cell-mediated antibody-dependent-cellular-cytotoxicity-(ADCC) mechanism, colorectal carcinoma (CRC) cells can still escape immunosurveillance. To overcome this limitation, we used panitumumab to redirect Fcγ chimeric receptor (CR) T against...