A5037562567- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Bacterial Infections and Vaccines
- Influenza Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Pneumocystis jirovecii pneumonia detection and treatment
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Image Enhancement Techniques
- Lung Cancer Research Studies
- Antibiotic Resistance in Bacteria
- Viral Infections and Vectors
- Pleural and Pulmonary Diseases
- Evolution and Genetic Dynamics
- Insect symbiosis and bacterial influences
- COVID-19 diagnosis using AI
- SARS-CoV-2 detection and testing
- Vector-borne infectious diseases
- Complement system in diseases
- Virology and Viral Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- COVID-19 epidemiological studies
National Institute for Public Health and the Environment
2020-2025
Wilhelmina Children's Hospital
2020-2024
University Medical Center Utrecht
2015-2024
Wageningen University & Research
2016
Amsterdam UMC Location University of Amsterdam
2014
Introduction Streptococcus pneumoniae is a major cause of community-acquired pneumonia and invasive diseases. Vaccination prevents pneumococcal disease caused by vaccine serotypes but leads to an increase in non-vaccine serotypes. Aim We aimed characterise serotype 38 isolates from (IPD) patients Germany, Poland the Netherlands explain recent surge cases. Methods IPD surveillance data 2013/2014 2023/2024 were reviewed all three countries for trends incidence. Collected serotyped...
Streptococcus pneumoniae causes significant morbidity and mortality among older adults. Detection of pneumococcal carriage is an accepted endpoint in conjugate vaccine studies. However, low sensitivity culture-based approaches nasopharyngeal samples have hampered adult S. studies the past. In contrast, detection carriers with qPCR-based can achieve high specificity testing oral improves accuracy detection. this Viewpoint we outline a strategy for accurate testing. We recommend dual-target...
Borrelia miyamotoi is a relapsing fever spirochete found in Ixodes ticks North America, Europe, and Asia, has recently been to be invasive humans. Cultivation of this not yet described, but important for patient diagnostics scientific purposes. Host specificity species dependent on resistance host complement (serum resistance), since B. identified as human pathogen we were interested whether resistant complement. We inoculated strains LB-2001 HT31 modified-Kelly-Pettenkofer medium with 10%...
In older adults, pneumococcal disease is strongly associated with respiratory viral infections, but the impact of viruses on Streptococcus pneumoniae carriage prevalence and load remains poorly understood. Here, we investigated effects influenza-like illness (ILI) in community-dwelling adults.We presence DNA saliva samples collected 2014/2015 influenza season from 232 individuals aged ≥60 years at ILI onset, followed by sampling 2-3 weeks 7-9 after first sample. We also sampled 194...
Despite strong historical records on the accuracy of saliva testing, oral fluids are considered poorly suited for pneumococcal carriage detection. We evaluated an approach surveillance and vaccine studies that increases sensitivity specificity pneumococcus serotype detection in samples.Quantitative PCR (qPCR)-based methods were applied to detect serotypes 971 samples collected from 653 toddlers 318 adults. Results compared with culture-based qPCR-based nasopharyngeal children oropharyngeal...
Abstract Background Understanding the dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) household transmission is important for adequate infection control measures in this ongoing pandemic. Methods Households were enrolled upon a polymerase chain reaction–confirmed index case between October and December 2020, prior to disease 2019 vaccination program. Saliva samples obtained by self-sampling at days 1, 3, 5, 7, 10, 14, 21, 28, 35, 42 from study inclusion....
Background The specificity of molecular methods for the detection Streptococcus pneumoniae carriage is under debate. We propose a procedure surveillance and vaccine impact studies that increases accuracy live pneumococci in polymicrobial respiratory samples. Methods Culture qPCR were applied to detect pneumococcus pneumococcal serotypes 1,549 nasopharyngeal samples collected Netherlands ( n = 972) England 577) from 946 toddlers 603 adults, paired oropharyngeal exclusively 319 Dutch adults....
Abstract Carriage of Neisseria meningitidis is an accepted endpoint in monitoring meningococcal vaccines effects. We have assessed N. and vaccine-type genogroup carriage prevalence college students at the time MenACWY vaccine introduction Netherlands, evaluated feasibility saliva sampling for surveillance carriage. For this, paired oropharyngeal samples collected from 299 were cultured meningococcus. The DNA extracted all bacterial growth was subjected to qPCRs quantifying genogroup-specific...
BackgroundNeisseria meningitidis is a commensal bacterium which can cause invasive disease. Colonisation studies are important to guide vaccination strategies.AimThe study's aim was determine the prevalence of meningococcal colonisation, duration carriage and distribution genogroups in Iceland.MethodsWe collected samples from 1 6-year-old children, 15-16-year-old adolescents 18-20-year-old young adults. Carriers were sampled at regular intervals until first negative swab. Conventional...
Streptococcus pneumoniae serotype 19A prevalence has increased after the implementation of PCV7 and PCV10 vaccines. In this study, we have provided, with high accuracy, genetic diversity in a cohort Dutch invasive pneumococcal disease patients asymptomatic carriers obtained period from 2004 to 2016. The whole genomes 338 isolates were sequenced their capsule (
ABSTRACT Background To characterize interferences between Streptococcus pneumoniae and SARS-CoV-2 we investigated the longitudinal patterns of viral infection pneumococcal carriage in households infected with SARS-CoV-2. Methods pneumococcus were detected quantitative molecular methods saliva from members eighty participating households. Samples collected October 2020 January 2021 n=197 adults n=118 children which n=176 n=98 had a complete set ten samples within 42 days since enrolment....
ABSTRACT Carriage of Neisseria meningitidis is an accepted endpoint in monitoring meningococcal vaccine effects. We applied molecular methods to assess the impact menACWY implementation on carriage and genogroup-specific prevalence young adults Fall 2022, four years after introduction tetravalent Netherlands. The overall rate genogroupable meningococci was not significantly different compared pre-menACWY cohort investigated 2018 (20.8% or 125 601 versus 17.4.% 52 299 individuals, p =0.25)....
Abstract Pneumococcal carriage studies have suggested that pneumococcal colonization in adults is largely limited to the oral cavity and oropharynx. In this study, we used total abundance-based β-diversity (dissimilarity) components characterize age-related differences serotype composition of respiratory samples. quantitative PCR (qPCR) was applied detect serotypes nasopharyngeal samples collected from 946 toddlers 602 adults, saliva a subset 653 toddlers, oropharyngeal 318 adults. Bacterial...
ABSTRACT Objectives Since conjugated polysaccharide vaccines reduce carriage of vaccine-type Neisseria meningitidis strains, meningococcal is an accepted endpoint in monitoring vaccine effects. We have assessed genogroup prevalence students at the time MenACWY introduction The Netherlands. In addition, we evaluated feasibility saliva sampling and qPCR-based detection method for surveillance carriage. Methods Paired oropharyngeal samples, collected from 299 students, were cultured...
ABSTRACT Background The specificity of molecular methods for the detection Streptococcus pneumoniae carriage is under debate. We propose a procedure that increases accuracy live pneumococci in polymicrobial respiratory samples. Methods Culture and qPCR were applied to detect S. pneumococcal serotypes 1549 nasopharyngeal samples collected Netherlands (n=972) England (n=577) from 946 toddlers 603 adults, paired oropharyngeal exclusively 319 Dutch adults. Samples with no isolated at primary...
Abstract Pneumococcal carriage studies have suggested that pneumococcal colonization in adults is largely limited to the oral cavity and oropharynx. In this study we used total abundance-based β-diversity (dissimilarity) components characterize age-related differences serotype composition of respiratory samples. qPCR was applied detect serotypes nasopharyngeal samples collected from 946 toddlers 602 adults, saliva a subset 653 toddlers, oropharyngeal 318 adults. Bacterial culture rates were...
ABSTRACT Background Complement-mediated killing is critical in the defense against meningococci. During a recent outbreak of invasive meningococcal serogroup W disease (IMD-W) Netherlands, predominant isolates belonged to clonal complex (cc) 11, which may suggest role for cc11-assocated traits complement resistance. We investigated resistance and carriage different serogroups lineages. In addition, we whether vaccine-induced antibodies can overcome complement-mediated killing. Methods...
Background: Understanding the dynamics of SARS-CoV-2 household transmission is important for adequate infection control measures in this ongoing pandemic. Methods: Households were enrolled upon a PCR-confirmed index case between October and December 2020, prior to COVID-19 vaccination programme. Saliva samples obtained by self-sampling at day 1, 3, 5, 7, 10, 14, 21, 28, 35, 42 from study inclusion. Nasopharyngeal swabs (NPS) oropharyngeal (OPS) collected research team 7 capillary blood 42....