A5100418709- Ubiquitin and proteasome pathways
- Semiconductor materials and devices
- Advancements in Semiconductor Devices and Circuit Design
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Alzheimer's disease research and treatments
- Healthcare Education and Workforce Issues
- Advanced Memory and Neural Computing
- Diverse Approaches in Healthcare and Education Studies
- Health and Wellbeing Research
- Protein Degradation and Inhibitors
- Genetics and Neurodevelopmental Disorders
- Urinary Bladder and Prostate Research
- Neuroscience and Neuropharmacology Research
- Pelvic floor disorders treatments
- Psychosocial Factors Impacting Youth
- Advanced Data Storage Technologies
- Integrated Circuits and Semiconductor Failure Analysis
- Infection Control and Ventilation
- Peptidase Inhibition and Analysis
- Education and Learning Interventions
- Cellular transport and secretion
- Cerebrospinal fluid and hydrocephalus
- Reconstructive Facial Surgery Techniques
- Urological Disorders and Treatments
Seoul National University
2015-2024
Chungnam National University Hospital
2024
Boramae Medical Center
2022-2023
Korea Testing Laboratory
2019-2023
Dongguk University
2023
The University of Melbourne
2018-2021
Chonbuk National University Hospital
2019-2021
Neuroscience Institute
2021
New Generation University College
2021
Seoul Institute
2021
Abstract When in the closed form, substrate translocation channel of proteasome core particle (CP) is blocked by convergent N termini α-subunits. To probe role gating mammalian proteasomes, we deleted N-terminal tail α3; resulting α3ΔN proteasomes are intact but hyperactive hydrolysis fluorogenic peptide substrates and degradation polyubiquitinated proteins. Cells expressing show markedly elevated many established resistance to oxidative stress. Multiplexed quantitative proteomics revealed...
Interior tertiary amine groups of PAMAM-OH dendrimers (hydroxyl-terminated polyamidoamine, PAMAM) were modified by methylation to make these polymers have a more cationic character, which enabled electrostatic interaction between and plasmid DNA. A reaction was dose-dependent, producing internally quaternized (QPAMAM-OH), thereby making amine/quaternary ratio adjustment possible. More highly condensed particles DNA formed as the degree quaternization increased, whereas unmodified polymer...
Highlights•Inactivation of USP14 results in downregulation autophagic flux•USP14 inhibition accelerates UVRAG degradation•Inhibition enhances proteasomal degradation tau/MAPT•USP14 is a common denominator UPS and autophagySummaryThe ubiquitin-proteasome system the autophagy-lysosome are two major intracellular proteolytic pathways eukaryotes. Although several biochemical mechanisms underlying crosstalk between them have been suggested, little known about effect enhanced proteasome activity...
Significance The ubiquitin–proteasome system and autophagy are two major intracellular proteolytic pathways, both remove misfolded proteotoxic proteins from eukaryotic cells. This study describes the detailed regulatory pathway of proteasome degradation by for its own quality control. We discovered that a portion inhibited proteasomes is actively sequestered into aggresome, an insoluble fraction mammalian cell. aggresome functions as triage point recovery autophagic degradation. mainly...
Abstract Proteolytic activity is an important virulence factor for Candida albicans (C. albicans). It attributed to the family of secreted aspartic proteinases (Saps) from C. with a minimum 10 members. Saps show controlled expression and regulation individual stages infection process. Distinct isoenzymes can be responsible adherence tissue damage local infections, while others cause systemic diseases. Earlier, only structures Sap2 Sap3 were known. In our research, we have now succeeded in...
Abstract The ubiquitin-proteasome system (UPS) is the primary mechanism by which intracellular proteins, transcription factors and many proteotoxic proteins with aggregation-prone structures are degraded. UPS reportedly downregulated in various neurodegenerative disorders, increased proteasome activity shown to be beneficial related disease models. Proteasomes function under tonic inhibitory conditions, possibly via ubiquitin chain-trimming of USP14, a proteasome-associated deubiquitinating...
Low-fluence 1064 nm Q-switched Nd:YAG laser has recently been shown to be effective for the melasma treatment.The purpose of this study is evaluate clinical efficacy and safety low-fluence treatment in Asian patients.Fifty patients with underwent 15 weeks weekly treatments, using a (RevLite®; HOYA ConBio®, Freemont, CA, USA) at an 8-mm spot size, fluence 2.8 J/cm(2). Patients investigators subjectively evaluated intensity pigmentation after completion treatments. The objective assessment was...
Tau is a cytosolic protein that functions in the assembly and stabilization of axonal microtubule networks. Its oligomerization may be rate-limiting step insoluble aggregate formation, which neuropathological hallmark Alzheimer's disease (AD) number other tauopathies. Recent evidence indicates soluble tau oligomers are toxic species for tau-mediated pathology during AD progression. Herein, we describe novel RNA aptamers target human were identified through an vitro selection process. These...
The tau protein is a highly soluble and natively unfolded protein. Under pathological conditions, undergoes multiple post-translational modifications (PTMs) conformational changes to form insoluble filaments, which are the proteinaceous signatures of tauopathies. To dissect crosstalk among PTMs during aggregation process, we phosphorylated ubiquitylated recombinant in vitro using GSK3β CHIP, respectively. resulting phospho-ub-tau contained conventional polyubiquitin chains with lysine 48...
The liver X receptors (LXRs) have been known as sterol sensors that impact cholesterol and lipid homeostasis, well inflammation. Although the hepatic functions of LXRs are documented, whether how play a pathophysiological role in lung remain largely unknown. Here we show LXRalpha LXRbeta expressed both type I II mouse epithelial cells, human cancer cells. To study vivo including pulmonary function this LXR isoform, created knock-in (LXR-KI) mice which constitutively activated (VP-LXRalpha)...
Until recently, the removal of melanocytic nevi has been performed with a CO(2) laser or Er:YAG laser. These lasers have useful for removing affected spots. However, enlargement spots some sequelae, including depressed hypertrophic scars, could develop as unwanted results. The Q-switched Nd:YAG used to remove deep-seated melanocytes, such Ota nevus tattoos. there no previous experiments test efficacy and safety this treatment nevi.The objective study was investigate 1,064 nm nevi, congenital...
To quantify aerosol generation from respiratory interventions and the effectiveness of their removal by a personal ventilation hood.Determination particle (in single, healthy volunteer in clean room) associated with breathing, speaking, wet coughing, oxygen (O2) 15 L/min via face mask, O2 60 nasal prongs, bilevel non-invasive positive-pressure (BiPAP) nebulisation 10 L/min.Aerosol was measured two sizer counter devices, focusing on aerosols 0.5-5 μm (human-generated aerosols), without hood....
In eukaryotes, a primary protein quality control (PQC) process involves the destruction of conformationally misfolded proteins through ubiquitin-proteasome system. Because approximately one-third eukaryotic proteomes fold and assemble within endoplasmic reticulum (ER) before being sent to their destinations, ER plays crucial role in PQC. The specific functions biochemical roles several E3 ubiquitin ligases involved ER-associated degradation mammals, on other hand, are mainly unknown. We...
A two-staged traffic control scheme, in which sets of candidate paths are prepared off-line prior to overall motion planning process, has been widely adopted for mobile robots, but relatively little attention given the application scheme multiple automated guided vehicle systems (MAGVSs). In paper, a systematic is presented obtain collision-free minimum-time motions AGVs along loopless paths. The structure controller divided into two tandem modules routing table generator (RTG) and an online...
Abstract Background: Serology is widely used for epidemiologic research of Helicobacter pylori . However, there limited information on the long‐term follow up H. titers after eradication. In addition, it presumed that reinfection rate decreases as infection decreases. The aim this study was to investigate IgG, and evaluate in Korea. Methods: Among 247 patients, who were enrolled during 2003–07, 185 patients with invasive test positive received proton pump inhibitor‐based triple therapy,...
Abstract In tauopathy conditions, such as Alzheimer's disease (AD), highly soluble and natively unfolded tau polymerizes into an insoluble filament; however, the mechanistic details of this process remain unclear. brains AD patients, only a minor segment forms β‐helix‐stacked protofilaments, while its flanking regions form disordered fuzzy coats. Here, it is demonstrated that nucleation core (tau‐AC) sufficiently induced self‐aggregation recruited full‐length to filaments. Unexpectedly,...