Long non-coding RNAs (lncRNAs) have been studied extensively over the past few years. Large numbers of lncRNAs identified in mouse, rat, and human, some them shown to play important roles muscle development myogenesis. However, there are reports on characterization covering all stages skeletal livestock. RNA libraries constructed from developing longissimus dorsi fetal (45, 60, 105 days gestation) postnatal (3 after birth) goat (Capra hircus) were sequenced. A total 1,034,049,894 clean reads...

Uncoupling protein 1 (UCP1) is a crucial located in the mitochondrial inner membrane that mediates nonshivering thermogenesis. However, molecular mechanisms by which enhancer–promoter chromatin interactions control Ucp1 transcriptional regulation brown adipose tissue (BAT) are unclear. Here, we employed circularized chromosome conformation capture coupled with next-generation sequencing (4C-seq) to generate high-resolution interaction profiles of interscapular (iBAT) and epididymal white...

Abstract Hu sheep (HS), a breed of carrying the FecB mutation gene, is known for its “year-round estrus and multiple births” an ideal model studying high fecundity mechanisms livestock. Through analyzing comparing genomic selection features other breeds, we identified series candidate genes that may play role in sheep’s mechanisms. In this study, conducted whole-genome resequencing on six breeds screened key mutations significantly correlated with reproductive traits sheep. Notably, CC2D1B...

Adaptive thermogenesis by brown adipose tissue (BAT) is important to the maintenance of temperature in newborn mammals. Cold exposure activates gene expression and lipid metabolism provide energy for BAT thermogenesis. However, knowledge large animals after cold still limited.In this study, we found that induced genes increased protein levels UCP1 PGC1α. Pathway analysis showed activated metabolism, which involved cGMP-PKG, TCA cycle, fatty acid elongation, degradation pathways. These were...

β-1,4-N-acetylgalactosamine transferase 2 (B4GALNT2) is a vital candidate gene that affects the growth traits in sheep. However, whether it has same function goats remains to be investigated further. This study selected 348 Nanjiang Yellow goats, screened all exons, and conserved non-coding regions of B4GALNT2 for single-nucleotide polymorphisms (SNPs). Our results revealed presence synonymous mutation, rs672215506, within exon goat population. The mutation resulted decrease mRNA stability...

Background Optimizing buck semen preservation techniques can significantly advance the goat industry. This study aimed to investigate effects of chitosan on sperm quality and seminal plasma metabolite profiles in bucks during low-temperature storage at 4°C. Results The results showed that when 0.2 mg/mL was added dilution, viability antioxidant capacity were highest higher than control group ( p < 0.05). Sperm decreased progressively with increasing time However, day 5, all groups...

Brown adipose tissue (BAT) has a significant impact in newborn goats on maintaining body temperature through non-shivering thermogenesis response to cold exposure. However, the roles of heat treatment BAT are still limited. This study focused effects short-term exposure goat brown adipocytes. We found that content mitochondria and proteins UCP1 PGC1α were increased after 12 h Additionally, triglyceride (TG) was significantly decreased 1, 2, 6 Furthermore, RNA-seq analysis adipocytes...

Tibetan goats, Taihang Jining grey and Meigu goats are the representative indigenous in China, found Qinghai-Tibet Plateau, Western pastoral area, Northern Southern agricultural regions. Very few studies have conducted a comprehensive analysis of genomic diversity selection these breeds. We genotyped 96 unrelated individuals, using goat 53 K Illumina BeadChip array, following breeds: (TG), (THG), (JGG), (MGG). A total 45 951 single nucleotide polymorphisms were filtered to estimate genetic...

The development of mammalian skeletal muscle is a highly complex process involving multiple molecular interactions. As prevalent RNA modification, N6-methyladenosine (m6A) regulates the expression target genes to affect development. Nevertheless, it remains unclear how m6A participates in goat muscle. In this study, methyltransferase 3 (METTL3) was significantly enriched longissimus dorsi (LD) tissue. addition, global modification level and differentiation satellite cells (MuSCs) were...

Skeletal muscle development is a complex biological process regulated by numerous genes and non-coding RNAs, such as microRNAs (miRNAs). In the current study, we made use of deep sequencing data from Jianzhou Da'er goat longissimus dorsi sampled on days 45, 60, 105 gestation, well day three after birth to identify miRNAs that regulate skeletal myogenesis, examine their temporal expression profiles. A total 410 known miRNAs, 752 miRNA homologs 88 novel were identified across four stages....

Abstract Background MicroRNAs (miRNAs) are a family of short non-coding RNA molecules and play important roles in various biological processes. However, knowledge the expression profiles function miRNAs on regulation brown adipose tissue (BAT) thermogenesis remains largely unknown. Results In this study, we found that existed within perirenal fat at 1 day after birth (D1) transferred into white (WAT) 30 days (D30) by UCP1 protein immunohistochemistry analysis. After that, performed...

Understanding the genetic composition of indigenous goats is essential to promote scientific conservation and sustainable utilization these breeds. The Jianchang Black (JC) goat, a Chinese native breed, solid black exhibits crude feed tolerance, but characterized by low growth rate small body size. Based on whole-genome sequencing data for 30 JC, 41 Jintang (JT), 40 Yunshang (YS) goats, 21 Bezoar ibexes, here, we investigated JC conducting analyses population structure, runs homozygosity...

Diarrhea is associated with gut microbiota, immunity, and metabolic alterations in goat kids lambs. This study used 28 lambs (11 healthy 17 diarrheic) 20 (10 10 to investigate the association between diarrhea occurrence changes metabolism, immunity The results revealed that Firmicutes, Proteobacteria, Bacteroidetes were dominant phyla In addition, Enterobacteriaceae Lachnospiraceae families identified both diarrheic Furthermore, functional prediction of microbiota showed it was involved cell...

Long noncoding RNAs (lncRNAs) have emerged as essential regulators of skeletal myogenesis, but few myogenesis-associated lncRNAs been identified and our understanding their regulatory mechanisms remains limited, particularly in goat. Here, we a novel lncRNA, TCONS_00006810 (named lncR-125b), from previous lncRNA sequencing data on fetal (45, 60, 105 days gestation, three biological replicates for each point) postnatal (3 after birth, n = 3) goat muscle, found that it is highly expressed...

Brown adipose tissue (BAT) plays an important role on no shivering thermogenesis during cold exposure to maintain animal body temperature and energy homeostasis. However, knowledge of the cellular transition from white (WAT) BAT is still limited. In this study, we provided a comprehensive metabolomics transcriptional signatures goat WAT. A total 157 metabolites were significantly changed, including 81 upregulated 76 downregulated metabolites. addition, identified citric acid cycle, fatty...

There is a long-term interest in investigating the genetic basis of horned/polled phenotype domestic goats. Here, we report genome-wide association study (GWAS) to detect loci affecting polled goats.We obtained total 13,980,209 biallelic SNPs, using genotyping-by-sequencing data from 45 Jintang Black (JT) goats, which included 32 female and nine male four individuals with intersex syndrome (PIS). Using mixed-model based GWAS, identified two signals, were located at 150,334,857-150,817,260 bp...

Myogenesis is a complex process controlled by several coding and non-coding RNAs (ncRNAs), such as circular (circRNAs) that are known to function endogenous microRNAs (miRNAs) sponges. Cerebellar Degeneration-Related protein 1 antisense (CDR1as) the most spotlighted circRNA an miR-7 sponge, which has bloomed circRNAs' research in animal disease physiology. Here, we screened for miRNAs mRNA associated with CDR1as further characterized their regulatory during muscle differentiation. We found...

The incidence of diarrhea in lambs is frequent large-scale sheep farms, which greatly impacts the growth and health lambs. aim this study was to assess changes serum biochemical immunological parameters gut microbiome composition suckling suffering from or not, reared on an intensive commercial farm. We found a reduced diversity intestinal bacteria diarrhea. Firmicutes Bacteroidetes were dominant flora both groups lambs, while decreased diarrheic no reported Firmicutes. Compared with healthy...

Domestic animals have multiple phenotypes of skin and coat color, which arise from different genes their products, such as proteins metabolites responsible with melanin deposition. However, the complex regulatory network synthesis remains to be fully unraveled. Here, tongue tissues Liangshan black sheep (black group) semi-fine-wool (pink were collected, stained hematoxylin–eosin (HE) Masson–Fontana, transcriptomic metabolomic data further analyzed. We found a large deposit granules in...

Long noncoding RNAs (lncRNAs) play an important role in the thermogenesis and energy storage of brown adipose tissue (BAT). However, knowledge cellular transition from BAT to white (WAT) potential lncRNAs goat remains largely unknown. In this study, we analyzed transformation WAT using histological uncoupling protein 1 (UCP1) gene analyses. Brown mainly existed within perirenal fat at day there was obviously a year. The RNA libraries constructed tissues day, 30 days, year goats were...