Abstract The treatment of bone defects remains a substantial clinical challenge due to the lack spatiotemporal management immune microenvironment, revascularization, and osteogenic differentiation. Herein, deferoxamine (DFO)‐loaded black phosphorus nanosheets decorated by polydopamine layer are prepared (BPPD) compounded into gelatin methacrylate/sodium alginate methacrylate (GA) hybrid hydrogel as smart‐responsive therapeutic system (GA/BPPD) for accelerated regeneration. BPPD...

Ideal periosteum materials are required to participate in a sequence of bone repair-related physiological events, including the initial immune response, endogenous stem cell recruitment, angiogenesis, and osteogenesis. However, conventional tissue-engineered periosteal have difficulty achieving these functions by simply mimicking via structural design or loading exogenous cells, cytokines, growth factors. Herein, we present novel biomimetic preparation strategy comprehensively enhance...

Background:The comprehensive management of diabetic bone defects remains a substantial clinical challenge due to the hostile regenerative microenvironment characterized by aggravated inflammation, excessive reactive oxygen species (ROS), bacterial infection, impaired angiogenesis, and unbalanced homeostasis.Thus, an advanced multifunctional therapeutic platform capable simultaneously achieving immune regulation, elimination, tissue regeneration is urgently designed for augmented under...

Factor-free biomaterial scaffolds play an increasingly important role in promoting

Previous studies have reported that adenosine monophosphate‑activated protein kinase (AMPK) activation can enhance osteoblast differentiation and mineralization; however, the underlying mechanism is not fully understood. Autophagy also serves an important role in mineralization bone homeostasis. The present study aimed to explore whether of AMPK could via induction autophagy. fracture healing nonunion animal models were established verified by X-ray imaging. Bone maturation was measured...

Biomimetic organic-inorganic nanocomposite scaffolds represent a tremendous opportunity to accelerate bone regeneration due their excellent structural and biological cues. However, the tradeoffs between biodegradability, mechanical strength, porosity pore size, bioactivity of tissue-engineered nanocomposites remain challenge. To this end, we constructed novel biodegradable scaffold by chemical crosslinking hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) montmorillonite (MMT)...

Osteogenesis and osteoclastogenesis are closely associated during the bone regeneration process. The development of multifunctional repair scaffolds with dual therapeutic actions (pro-osteogenesis anti-osteoclastogenesis) is still a challenging task for tissue engineering applications. Herein, through facile surface coating process, mussel-inspired polydopamine (PDA) adhered to biocompatible porous scaffold followed by immobilization small-molecule activator (LYN-1604 (LYN)) subsequent in...

Bioinspired materials with dual capabilities of pro-osteogenesis and pro-angiogenesis have attracted increasing scientific interest in the field orthopedics. Herein, inspired by mineralization process natural bone, a biomimetic strategy is presented to fabricate highly bioactive hybrid nanocomposite scaffolds via impregnation Si-containing polymer-induced liquid precursor (PILP) into biocompatible porous gelatin followed situ free radial polymerization. The well-designed biohybrid scaffold...

Acquisition of drug-resistant phenotypes is often associated with chemotherapy in osteosarcoma. A number studies have demonstrated a critical role for autophagy osteosarcoma development, therapy and drug resistance. However, the molecular mechanisms underlying autophagy-mediated resistance cells remain largely unknown. In present study, we determined microRNA-140 (miR-140-5p, miRBase ID: MIMAT0000431) expression induced by chemotherapeutic drugs cells. Then promotory miR-140-5p to...

Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration. Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed this study the of sustainable release icariin subsequent regeneration.An icariin-loaded scaffold was prepared characterized by SEM HPLC morphology behavior, respectively. The mechanical properties, degradation PBS cytotoxicity on BMSCs also evaluated MTT assay,...

Abstract Inspired by natural redwood and bone, a biomimetic strategy is presented to develop highly bioactive redwood‐like nanocomposite via radial freeze casting of biocompatible hydrogels followed the in situ coprecipitation Si‐containing CaP hybrid nanocoating (SCPN). The engineered material displays radially aligned macrochannels porous network structure similar those redwood. In addition acting as mechanical reinforcement, introducing SCPNs into weak scaffold yields not only...

The reconstruction of large bone defects by bioactive materials without exogenous cells, cytokines or growth factors remains a substantial clinical challenge. Herein, we present the rational design injectable and adhesive (GMAD/LP) hydrogels composed gelatin-methacryloyl (GelMA), dopamine-grafted alginate (AD), polydopamine-functionalized Laponite ([email protected]) nanosheets that can act as osteoimmune regulators to engineer pro-regenerative microenvironment for personalized regeneration....

Osteosarcoma patients with lung metastasis and local invasion remain challenging to treat despite the significant contribution of combination surgery neo-adjuvant chemotherapy. Our previous microarray study demonstrated that miR-302b had significantly lower expression in osteosarcoma cell lines than osteoblast lines. In present study, we further elucidated role regulating migration invasiveness osteosarcoma. MiR-302b was markedly down-regulated clinical tumour tissues. Lower levels were...

This study aimed to examine the expression of and relationship between CD44V6, CDH11, β -catenin. The these cell adhesion molecules was detected in 90 osteosarcoma 20 osteochondroma specimens using immunohistochemistry. Associations parameters clinicopathological data were also examined. rates -catenin 25.0% (5/20), 70.0% (14/20), 20.0% (4/20) specimens, respectively. Compared osteochondromas, proportions CD44V6 increased 65.6% (59/90) 60.0% (54/90), However, rate CDH11 osteosarcomas reduced...

Non-union of the tibia complicated by osteomyelitis is one most challenging problems in orthopaedic surgery. There remains a significant amount debate and controversy regarding optimal medical management infected tibial non-union. are few articles which have reported outcomes treatment for non-union from single-stage reconstruction with open bone grafting plus vacuum-assisted closure (VAC).Our report covers experience between March 2007 February 2010 VAC stage patients The time union wound...

Background:The expression of Src and phospho-Src (p-Src) is closely related to tumor invasion metastasis. The aim the present study was investigate these molecules in osteosarcoma their relationship with each other, provide a theoretical basis understand prognosis osteosarcoma. Material andMethods:We selected surgically resected specimens from 116 patients Zhongnan Hospital Wuhan University Hubei Cancer Hospital, Hubei, China, between January 2000 2010 detailed follow-up data. Twenty...

This article aimed to explore whether hook plate implantation significantly narrowed the subacromial space. 24 shoulder joints were implanted with clavicle plates. A Vernier caliper was used measure length and thickness of acromion. In addition, measured. The data from all measurements recorded divided into 2 groups Group A, group: acromion hook, B, depth hook. There are significant statistical differences when comparing between subgroups within each group. exceeds (12.41±2.80 mm vs....

Salinomycin, a naturally occurring polyether ionophore antibiotic isolated from Streptomyces albus, has been demonstrated potent cytotoxic activity against variety of cancer cell lines. In particular, it exhibits selective targeting stem cells. However, systemic toxicity, drug resistance and low bioavailability the significantly limit its potential applications. this study, C20-epi-isothiocyanate salinomycin was designed synthesized, then reacted with amines as versatile synthon to assemble...

To investigate the influence of vacuum sealing drainage on sciatic nerve repair after injury in rabbits.Twenty four New Zealand white rabbits were randomly divided into experimental group and control group. About 1 cm was transected sutured back situ. The had assisted wound closure whereas normal closure. rate compared based conduction velocity, lower leg triceps wet weight recovery rate, histology, immunohistochemical brain-derived neurotrophic factor, ultrastructure observation regenerated...