Deficient bone regeneration causes defects or nonunion in a substantial proportion of trauma patients that urges for novel therapies. To develop reliable therapy, we investigated the effect negative pressure wound therapy (NPWT) on vivo rat calvarial defect model. Negative (NP) treatment vitro was mimicked to test its osteoblast differentiation mesenchymal stem cells (MSCs) and MC3T3-E1 cells. Transcriptomic analyses, pharmaceutical interventions, shRNA knockdowns were conducted explore...

5-methylcytosine (m5C) modification, which is mainly induced by the RNA methyltransferase NSUN2 (NOP2/Sun domain family, member 2), an important chemical posttranscriptional modification in mRNA and has been proven to play roles progression of many cancers. However, functions underlying molecular mechanisms NSUN2-mediated m5C osteosarcoma (OS) remain unclear. In this study, we found was highly expressed OS tissues cells. We also discovered that higher expression predicted poorer prognosis...

Abstract Phototheranostics has garnered sustained attention due to its significant potential for revolutionizing conventional cancer treatment strategies. While being one of the most commonly employed strategies constructing phototheranostic systems by engineering integration photosensitizers (PSs) into nanosystems, nano‐PSs face challenges including complexity in preparation process, low delivery efficiency, and toxicity issues. Contrastingly, burgeoning popularity small molecule PSs...

Abstract Osteoporosis (OP) results from the impaired function of endogenous bone marrow mesenchymal stem cells (BMSCs). Icariin (ICA) has shown potential osteoprotective effects. However, molecular mechanism for anabolic action ICA remains largely unknown. The objective present study is to investigate whether prevents loss by acting on BMSCs via affecting level autophagy after ovariectomy (OVX). were extracted BALB/c mice treated with ICA, chloroquine (CQ, an inhibitor) or + CQ. OVX injected...

Icariin (IC) promotes osteogenic differentiation, and it may be a potential small molecule drug for local application in bone regeneration. Icariin-loaded hydroxyapatite/alginate (IC/HAA) porous composite scaffolds were designed this study the of sustainable release icariin subsequent regeneration.An icariin-loaded scaffold was prepared characterized by SEM HPLC morphology behavior, respectively. The mechanical properties, degradation PBS cytotoxicity on BMSCs also evaluated MTT assay,...

Osteoporosis is characterized by excessive bone resorption and/or defects in formation. Identification of factors promoting osteoblast differentiation may provide potential targets for osteoporosis therapy. Through integral analyses multiple datasets, NIBAN2 found to be tightly associated with formation and osteoporosis. Indeed, promotes differentiation, conditional Niban2 knockout osteoblasts caused loss insufficient mineralization. Mechanistically, interacts the HNRNPU-cored spliceosome...

Osteosarcoma patients with lung metastasis and local invasion remain challenging to treat despite the significant contribution of combination surgery neo-adjuvant chemotherapy. Our previous microarray study demonstrated that miR-302b had significantly lower expression in osteosarcoma cell lines than osteoblast lines. In present study, we further elucidated role regulating migration invasiveness osteosarcoma. MiR-302b was markedly down-regulated clinical tumour tissues. Lower levels were...

Aim: We attempted to synthesize a magnetic gene carrier with poly(ethylenimine), dextran and iron oxide nanoparticles (PDIs) for miR-302b transfection in vitro vivo. Materials & methods: The were characterized hydrodynamic properties, ζ potential DNA-binding ability, evaluated by transmission electron microscopy. Cellular internalization, magnetofection efficiency anti-osteosarcoma effects investigated osteosarcoma (OS) cells OS-bearing nude mice. Results: PDIs successfully prepared showed...

Minimally invasive surgery will be gradually applied to the surgical treatment of bone tumors. One difficulties in minimally tumors is lack injectable materials that can used treat tumor-induced defects. Therefore, it imperative develop an filler not only injected into defect site by methods provide strong support and repair tissue but also inactivate residual tumor cells around defect. To achieve this aim, our study, for first time, we doped Fe3O4/graphene oxide (GO) nanocomposites...

Skin wounds caused by external injuries remain a serious challenge in clinical practice. Wound dressings that are antibacterial, pro-angiogenic, and have potent regeneration capacities highly desirable for wound healing. In this study, minimally invasive wound-friendly Cu@ZIF-8 encapsulated PEGDA/CMCS microneedle (MN) array was fabricated using the molding method to promote The MNs had good biocompatibility, excellent mechanical strength, as well strong antibacterial properties...

Chemoresistance remains a huge challenge for the treatment of osteosarcoma (OS). Multiple mechanisms are involved in chemoresistance cisplatin. In our research, we found that Nrf2/xCT pathway contributes to occurrence cisplatin OS by enhancing ability antioxidative stress. Recently, oxidative stress defense and ferroptosis have attracted wildly attention overcoming chemoresistance. Different from apoptosis, necroptosis, autophagy, is new programmed cell death with accumulation reactive...

Abstract Background Functional electrospun membranes are promising dressings for promoting wound healing. However, their microstructure and drug loading capacity need further improvements. It is the first time to design a novel mesh-like fiber loaded with atorvastatin (ATV) investigated its effects on paracrine secretion by bone marrow-derived mesenchymal stem cells (BMSCs) healing in vivo. Methods We fabricated membrane using copper mesh receiver. The physical properties of were evaluated...

Abstract The vast majority of giant-cell tumors occur around the knee and characteristically affect subchondral bone. Thermal damage to articular cartilage arising from application polymethylmethacrylate (PMMA) or extensive intralesional curettage presents a challenging problem orthopedic surgeons patients due compliance issues. For this reason, we developed new bone-grafting procedure restore massive bone defects reduce degenerative changes in knee. aim study was describe novel evaluate...

Abstract Background and Objectives Negative surgical margins are significant in improving patient outcomes. However, surgeons can only rely on visual tactile information to identify tumor intraoperatively. We hypothesized that intraoperative fluorescence imaging with indocyanine green (ICG) could serve as an assistive technology evaluate guide surgery bone soft tissue surgery. Methods Seventy patients tumors were enrolled this prospective, non‐randomized, single‐arm feasibility study. All...

Background:The expression of Src and phospho-Src (p-Src) is closely related to tumor invasion metastasis. The aim the present study was investigate these molecules in osteosarcoma their relationship with each other, provide a theoretical basis understand prognosis osteosarcoma. Material andMethods:We selected surgically resected specimens from 116 patients Zhongnan Hospital Wuhan University Hubei Cancer Hospital, Hubei, China, between January 2000 2010 detailed follow-up data. Twenty...

Dysregulated osteoclast activity due to altered differentiation causes multiple bone diseases. Osteoclasts are multinucleated giant cells derived from hematopoietic stem and play a major role in absorption. However, the mechanisms underlying tight regulation of pathophysiological status remain unknown.We showed that Bhlhe40 upregulation is tightly associated with osteoporosis. Functionally, promoted vitro, deficiency led increased mass decreased vivo. Moreover, deficient mice resisted...

Abstract Objective The purpose of this study is to explore the effect miRNA-539 on osteosarcoma (OS) and underlying mechanism, so as find a new method for early diagnosis treatment osteosarcoma. Method mimics was transfected into cells 143b MG-63 upregulated expression miR-539. QT-PCR used detect transfection efficacy. CCK-8 proliferation flow cytometry apoptosis MG-63. Wound-healing test Transwell were migration invasion ability cells. TRIAP1 found be potential target gene by online...