A5048689885- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Tumors and Oncological Cases
- Salivary Gland Tumors Diagnosis and Treatment
- Liver physiology and pathology
Synthetic Genomics (United States)
2020
Massachusetts General Hospital
2017-2019
Harvard University
2017-2018
Georgia Institute of Technology
2018
Chestnut Hill College
2017
Boston College
2017
Significance The early detection of hepatocellular carcinoma (HCC) is paramount importance for improving patient outcomes, yet an accurate, high-throughput screening methodology has to be developed. By combining microfluidic depletion hematopoietic cells from blood specimens with absolute quantification lineage-derived transcripts, we demonstrate the highly specific circulating tumor cells, enabling noninvasive and clinical monitoring HCC.
Abstract Background Recent work has demonstrated early shedding of circulating epithelial cells (CECs) from premalignant intraductal papillary mucinous neoplasms (IPMNs). However, the potential use CECs as a “liquid biopsy” for patients with IPMNs been limited by antigen dependence CEC isolation devices and lack robust detection biomarkers across phenotypes. Materials Methods We utilized negative depletion microfluidic platform to purify contaminating leukocytes coupled this...
Single cell technologies have described heterogeneity across tissues, but the spatial distribution and forces that drive single phenotypes not been well defined. Combining RNA protein analytics in pancreatic cancer (PDAC) model systems, we demonstrated role of stromal fibroblasts shaping PDAC towards invasive (EMT) proliferative (PRO) phenotypes. Using highcontent digital imaging situ hybridization 195 tumors, observed these EMT PRO subpopulations 319,626 individual cells. Interestingly,...
Historically poor clinical results of tumor vaccines have been attributed to weakly immunogenic antigen targets, limited specificity, and vaccine platforms that fail induce high-quality polyfunctional T cells, central mediating cellular immunity. We show here the combination selection, construct design, a robust platform based on Synthetically Modified Alpha Replicon RNA Technology (SMARRT), self-replicating RNA, leads control growth in mice. Therapeutic immunization with SMARRT...
Microfluidic platforms have demonstrated the ability to isolate rare circulating tumor cells from a wide variety of cancers. Our group has recently shown (CTCs) hepatocellular carcinoma (HCC) patients using hematopoietic cell depletion microfluidic platform. However, relationship CTC generation and HCC progression is still not well understood. Tumor size often used as clinical prognostic factor, but there been an inconsistent metastatic recurrence. Characterizing primary CTCs would provide...
Abstract Background: Although liver cancer has the second-highest mortality rate among cancers internationally, accurate and scalable assays for early detection longitudinal monitoring of hepatocellular carcinoma are lacking. Circulating tumor cells released from invasive into blood stream, but difficulty inherent in isolating, identifying, characterizing these ultra-rare precluded their widespread implementation as a biomarker. By combining high-throughput microfluidic negative depletion...