A5010464197- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- COVID-19 and Mental Health
- Insect Resistance and Genetics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Telemedicine and Telehealth Implementation
- Endoplasmic Reticulum Stress and Disease
- Sarcoma Diagnosis and Treatment
- Pancreatic function and diabetes
- Insect Utilization and Effects
- Bacterial Genetics and Biotechnology
- Mental Health Treatment and Access
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Migration, Health and Trauma
- Diet, Metabolism, and Disease
- Genetic Associations and Epidemiology
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
Icahn School of Medicine at Mount Sinai
2018-2025
Massachusetts General Hospital
2018-2020
Harvard University
2019-2020
Massachusetts Institute of Technology
2017
To explore the developmental reorganization of three-dimensional genome brain in context neuropsychiatric disease, we monitored chromosomal conformations differentiating neural progenitor cells. Neuronal and glial differentiation was associated with widespread remodeling contact map included interactions anchored common variant sequences that confer heritable risk for schizophrenia. We describe cell type-specific connectomes composed schizophrenia variants their distal targets, which...
Transcriptional profiling has defined pancreatic ductal adenocarcinoma (PDAC) into distinct subtypes with the majority being classical epithelial (E) or quasi-mesenchymal (QM). Despite clear differences in clinical behavior, growing evidence indicates these exist on a continuum features of both present and suggestive interconverting cell states. Here, we investigated impact different therapies evaluated PDAC phenotypic spectrum E/QM state. We demonstrate using RNA-sequencing RNA-in situ...
Abstract Pancreatic ductal adenocarcinoma (PDAC) lethality is due to metastatic dissemination. Characterization of rare, heterogeneous circulating tumor cells (CTCs) can provide insight into metastasis and guide development novel therapies. Using the CTC-iChip purify CTCs from PDAC patients for RNA-seq characterization, we identify three major correlated gene sets, with stemness genes LIN28B/KLF4 , WNT5A LGALS3 enriched in each set; only LIN28B CTC expression was prognostic. CRISPR knockout...
Downstream metabolic events can contribute to the lethality of drugs or agents that interact with a primary cellular target. In bacteria, production reactive oxygen species (ROS) has been associated lethal effects variety stresses including bactericidal antibiotics, but relative contribution this oxidative component cell death depends on factors. Experimental evidence suggested unresolvable DNA problems caused by incorporation oxidized nucleotides into nascent followed incomplete base...
In this study, we aimed to determine the efficacy of in vivo chimeric antigen receptor (CAR) T cell therapy, generated by targeted lipid nanoparticles (t-LNPs), as an anti-fibrotic metabolic dysfunction-associated steatotic liver disease. Hepatic fibrosis is a key predictor mortality disease, driven fibrogenic hepatic stellate cells (HSCs). heart, targeting fibroblast activation protein alpha (FAP) reduce murine cardiac fibrosis. However, value approach unknown. We explored potential...
The distinction of atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) from its benign counterpart, lipoma, may represent a challenge. MDM2 DNA amplification is used as the gold standard immunohistochemistry lacks specificity and sensitivity. Herein, we investigate diagnostic utility RNA in situ hybridization (RNA-ISH) compare test with fluorescence (FISH) malignant neoplasms. We evaluated 109 neoplasms including 27 lipomas, 25 spindle cell 32 ALTs/WDLs, dedifferentiated...
Single cell technologies have described heterogeneity across tissues, but the spatial distribution and forces that drive single phenotypes not been well defined. Combining RNA protein analytics in pancreatic cancer (PDAC) model systems, we demonstrated role of stromal fibroblasts shaping PDAC towards invasive (EMT) proliferative (PRO) phenotypes. Using highcontent digital imaging situ hybridization 195 tumors, observed these EMT PRO subpopulations 319,626 individual cells. Interestingly,...
Commercial protease enzymes are usually produced from bacteria due to their high rate of production and simple downstream processing. Alkaline proteases have the ability work efficiently in extreme industrial conditions environmental friendly. In recent years as an alternative chemicals, researchers trying find suitable enzymes. Hence aim this study was isolation purification alkaline serine midgut microbes Bombyx mori larva. Results revealed presence Gram positive short tubular with...