A5015044919- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Bioinformatics and Genomic Networks
- Epigenetics and DNA Methylation
- Alzheimer's disease research and treatments
- Single-cell and spatial transcriptomics
- Ubiquitin and proteasome pathways
- Genetic Associations and Epidemiology
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Neuroscience and Neural Engineering
- Muscle Physiology and Disorders
- DNA Repair Mechanisms
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- NF-κB Signaling Pathways
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Autophagy in Disease and Therapy
- Adolescent and Pediatric Healthcare
- Nuclear Receptors and Signaling
- Ion channel regulation and function
- ATP Synthase and ATPases Research
Icahn School of Medicine at Mount Sinai
2015-2023
Allen Institute for Brain Science
2015-2023
New York Stem Cell Foundation
2023
Amgen (United States)
2023
The Graduate Center, CUNY
2015
Sungkyunkwan University
2010-2014
Samsung (South Korea)
2010-2012
To explore the developmental reorganization of three-dimensional genome brain in context neuropsychiatric disease, we monitored chromosomal conformations differentiating neural progenitor cells. Neuronal and glial differentiation was associated with widespread remodeling contact map included interactions anchored common variant sequences that confer heritable risk for schizophrenia. We describe cell type-specific connectomes composed schizophrenia variants their distal targets, which...
Dual-function poly(L-lysine) (PLL) composites that function as antibacterial agents and promote the growth of human cell culture have been sought by researchers for a long period. In this paper, we report preparation new graphene derivative-PLL via electrostatic interactions covalent bonding between derivatives PLL. The resulting were characterized infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy. novel dual PLL composites, specifically activity...
Abstract Chromosomal instability (CIN) is a hallmark of cancer, caused by persistent errors in chromosome segregation during mitosis. Aggressive cancers like high-grade serous ovarian cancer (HGSOC) and triple-negative breast (TNBC) have high frequency CIN TP53 mutations. Here, we show that inhibitors the KIF18A motor protein activate mitotic checkpoint selectively kill chromosomally unstable cells. Sensitivity to inhibition enriched -mutant HGSOC TNBC cell lines with features, including...
Highlights•The efficacy of CRISPR-mediated transcript modulation varies between genes•gRNAs should be re-validated for each individual, cell type, and dCas9-effectorSummaryModulation transcription, either synthetic activation or repression, via dCas9-fusion proteins is a relatively new methodology with the potential to facilitate high-throughput up- downregulation studies gene function. Genetic neurodevelopmental disorders have identified growing list risk variants, including both common...
Abstract Neurodevelopmental disorders, such as autism spectrum disorders and schizophrenia, are complex with a high degree of heritability. Genetic studies have identified several candidate genes associated these including contactin-associated protein-like 2 ( CNTNAP2 ). Traditionally, in animal models or vitro , has been studied by genetic deletion transcriptional knockdown, which reduces the expression entire gene; however, it remains unclear whether mutations clinical settings sufficient...
Cell-cell interactions between muscle precursors are required for myogenic differentiation; however, underlying mechanisms largely unknown. Promyogenic cell surface protein Cdo functions as a component of multiprotein complexes containing other adhesion molecules, Boc, Neogenin and N-cadherin, mediates some signals triggered by cell-cell precursors. activates p38MAPK via interaction with two scaffold proteins JLP Bnip-2 to promote myogenesis. Akt signaling differentiation activation both...
We describe a CRISPR inhibition (CRISPRi) protocol to repress endogenous gene expression (e.g.,
SUMMARY To study the molecular mechanisms driving pathogenesis and identify novel therapeutic targets of late onset Alzheimer’s Disease (LOAD), we performed an integrative network analysis whole-genome DNA RNA sequencing profiling four cortical areas, including parahippocampal gyrus, across 364 donors spanning full spectrum LOAD-related cognitive neuropathological disease severities. Our analyses revealed thousands changes uncovered for first-time multiple neuron specific gene subnetworks...
ABSTRACT Dopaminergic neurons are critical to movement, mood, addiction, and stress. Current techniques for generating dopaminergic from human induced pluripotent stem cells (hiPSCs) yield heterogenous cell populations with variable purity inconsistent reproducibility between donors, hiPSC clones, experiments. Here, we report the rapid (5 weeks) efficient (~90%) induction of (iDANs) through transient overexpression lineage-promoting transcription factors combined stringent selection across...
Schizophrenia (SZ) is a highly heritable neuropsychiatric disorder. Nevertheless, the functional mechanisms through which large contribution of common genetic variation underlies this disorder remain unclear. GWAS and eQTL analyses have previously identified candidate genes, whose expression affected by SZ risk variants (SZ‐eQTL genes). We established genetics‐driven hiPSC‐based approach for prioritization validation genes associated with SZ. Using strategy, we prioritized one putative...