A5014774803- MicroRNA in disease regulation
- Electromagnetic Fields and Biological Effects
- Sarcoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- Spaceflight effects on biology
- Microbial Inactivation Methods
- Astrophysics and Cosmic Phenomena
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Particle physics theoretical and experimental studies
- Medical Imaging Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- 3D Printing in Biomedical Research
- Renal and related cancers
- Bone health and treatments
- Pancreatic and Hepatic Oncology Research
- Tissue Engineering and Regenerative Medicine
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- Neutrino Physics Research
- Cancer Immunotherapy and Biomarkers
- Liver physiology and pathology
- Radiomics and Machine Learning in Medical Imaging
University of Turin
2007-2024
Ospedale San Luigi Gonzaga
2024
Azienda Ospedaliera Citta' della Salute e della Scienza di Torino
2023
Institute of Forensic Science
2023
Massachusetts General Hospital
2013-2020
Harvard University
2013-2020
Metropolitana Milanese (Italy)
2018-2020
Howard Hughes Medical Institute
2013-2015
University of Hawaii Cancer Center
2015
Dana-Farber/Harvard Cancer Center
2014-2015
Circulating tumor cells (CTCs) are present at low concentrations in the peripheral blood of patients with solid tumors. It has been proposed that isolation, ex vivo culture, and characterization CTCs may provide an opportunity to noninvasively monitor changing patterns drug susceptibility individual as their tumors acquire new mutations. In a proof-of-concept study, we established CTC cultures from six estrogen receptor-positive breast cancer. Three five lines tested were tumorigenic mice....
Highlights•Pancreatic CTCs can be enriched with antigen-agnostic microfluidic technology•Single-cell RNA sequencing of pancreatic reveals three distinct CTC populations•Extracellular matrix genes are highly expressed in mouse and human CTCs•The extracellular protein SPARC contributes to tumor metastasisSummaryCirculating cells (CTCs) shed from primary tumors into the bloodstream, mediating hematogenous spread cancer distant organs. To define their composition, we compared genome-wide...
Cancer's alternative means to an end To stay alive and proliferating, tumor cells must maintain their telomeres: the DNA sequences at ends of chromosomes. The majority accomplish this by activating enzyme telomerase. However, certain types favor a different mechanism called lengthening telomeres (ALT), which involves recombination. Flynn et al. delineated molecular events that occur ALT-proficient studying function protein is altered mutation in these tumors. analysis revealed specific...
Many microRNAs (miRNAs), posttranscriptional regulators of numerous cellular processes and developmental events, are downregulated in tumors. However, their role tumorigenesis remains largely unknown. In this work, we examined the muscle-specific miRNAs miR-1 miR-206 human rhabdomyosarcoma (RMS), a soft tissue sarcoma thought to arise from skeletal muscle progenitors. We have shown that was barely detectable primary RMS both embryonal alveolar subtypes failed be induced cell lines upon serum...
Significance Unique among the large number of noncoding RNA species, pericentromeric human satellite II (HSATII) repeat is massively expressed in a broad set epithelial cancers but nearly undetectable normal tissues. Here, we show that deregulation HSATII expression tightly linked to growth under nonadherent conditions, and uncover an unexpected mechanism by which RNA-derived DNA (rdDNA) leads progressive elongation regions tumors. The remarkable specificity overexpression cancers, together...
Insulin-like growth factor 2 ( IGF2 ), a developmentally regulated and maternally imprinted gene, is frequently overexpressed in pediatric cancers. Although loss of imprinting (LOI) at fetal promoters contributes to increased tumors, the magnitude expression suggests involvement additional regulatory mechanisms. A microRNA (miRNA) screen primary Wilms' tumors identified specific overexpression miR-483-5p, which embedded within gene. Unexpectedly, mRNA itself transcriptionally up-regulated by...
Abstract Rhabdomyosarcoma (RMS) is a highly malignant soft-tissue tumor of childhood deriving from skeletal muscle cells. RMS can be classified in two major histologic subtypes: embryonal (ERMS) and alveolar (ARMS), the latter being characterized by PAX3/7-FKHR translocation. Here we first investigated whether Met receptor, transcriptional target PAX3 PAX7, has role PAX3-FKHR–mediated transformation. Following PAX3-FKHR transduction, was up-regulated mouse fibroblasts (MEF), NIH 3T3 C2C12...
The effects of the exposure mitogen‐stimulated human lymphocytes from aged subjects to low‐frequency pulsed electromagnetic fields (PEMFs) were studied by measuring production interleukin‐2 (IL‐2) and expression IL‐2 receptor. PEMF‐exposed cultures that presented increased [ 3 H]thymidine incorporation showed lower amounts in their supernatants, but higher percentages receptor‐positive cells T‐activated lymphocytes. Taken together, these data suggest PEMFs able modulate mitogen‐induced...
The European Commission Implementing Decision EU 2015/495 included three steroidal estrogens, namely 17α-ethinyl estradiol, 17β-estradiol, and estrone, in the so-called "watch list" of Water Framework Directive (WFD). monitoring these compounds is difficult because detection limits majority available analytical methods cannot achieve very low target concentrations required to meet proposed environmental quality criteria. In 2014, a combined Science-Policy Interface/Chemical Monitoring...
Molecular drivers underlying bone metastases in human cancer are not well understood, part due to constraints tissue sampling. Here, RNA sequencing was performed of circulating tumor cells (CTC) isolated from blood samples women with metastatic estrogen receptor (ER)+ breast cancer, comparing cases progression versus visceral organs. Among the activated cellular pathways CTCs bone-predominant is androgen (AR) signaling. AR gene expression evident, as its constitutively active splice variant...
Abstract Modeling the hematogenous spread of cancer cells to distant organs poses one greatest challenges in study human metastasis. Both tumor cell–intrinsic properties as well interactions with reactive stromal contribute this process, but identification relevant signals has been hampered by lack models allowing characterization metastatic niche. Here, we describe an implantable bioengineered scaffold, amenable vivo imaging, ex manipulation, and serial transplantation for continuous...
Hydrodynamic Cavitation (HC) is considered as a promising water-disinfection technique. Due to the enormous complexity of physical and chemical processes at play, research on HC reactors usually carried out following an empirical approach. Surprisingly, past experimental studies have never been designed dimensional-analysis principles, which makes it difficult identify key controlling problem, isolate their effects scale up results from laboratory full-scale scenarios. The present paper...
We present an analysis of muon events with all multiplicities collected during 21804 h operation the first Large Volume Detector tower. The measured angular distribution intensity has been converted to "depth–vertical-intensity" relation in depth range from 3 12 km w.e. this allowed us derive power index γ primary all-nucleon spectrum: γ=2.78±0.05. standard rock and comparison data other experiments done. also derived vertical spectrum at sea level.Received April...
Zeni, O., Schiavoni, A. S., Sannino, A., Antolini, Forigo, D., Bersani, F. and Scarfì, M. R. Lack of Genotoxic Effects (Micronucleus Induction) in Human Lymphocytes Exposed In Vitro to 900 MHz Electromagnetic Fields. Radiat. Res. 160, 152–158 (2003).In the present study, we investigated induction genotoxic effects human peripheral blood lymphocytes after exposure electromagnetic fields used mobile communication systems (frequency MHz). For this purpose, incidence micronuclei was evaluated by...
Purpose: To study if prolonged in vitro exposure to 1800 MHz radiofrequency (RF) could exert an effect on human peripheral blood mononuclear cells (PBMC) from young and elderly donors by affecting apoptosis, mitochondrial membrane potential heat shock protein (HSP) 70 levels.Materials methods: Endpoints were analysed the presence or absence of apoptosis‐inducing agent 2‐deoxy‐D‐ribose. Three different signal modulations typical Global System for Mobile communication (GSM) system applied. The...
Rhadomyosarcoma (RMS) is the most common soft tissue sarcoma of childhood. RMS cells resemble fetal myoblasts but are unable to complete myogenic differentiation. In previous work we showed that miR-206, which low in RMS, when induced promotes resumption differentiation by modulating more than 700 genes. To better define pathways involved conversion into their differentiated counterpart, focused on 2 miR-206 effectors emerged from microarray analysis, SMYD1 and G6PD. SMYD1, one highly...
Current therapeutic options for the pediatric cancer rhabdomyosarcoma have not improved significantly, especially metastatic rhabdomyosarcoma. In current work, we performed a deep miRNA profiling of three major human subtypes, along with cell lines and normal muscle, to identify novel molecular circuits potential. The signature determined could discriminate from revealing subset muscle-enriched (myomiR), including miR-22, which was strongly underexpressed in tumors. miR-22 physiologically...