A5012307029- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Mesenchymal stem cell research
- Gestational Diabetes Research and Management
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Fatty Acid Research and Health
- Adipokines, Inflammation, and Metabolic Diseases
University of Colorado Anschutz Medical Campus
2021-2024
University of Michigan
2023
The adipose-derived hormone leptin acts via its receptor (LepRb) in the brain to control energy balance. A previously unidentified population of GABAergic hypothalamic LepRb neurons plays key roles restraint food intake and body weight by leptin. To identify markers for candidate populations an unbiased manner, we performed single-nucleus RNA-sequencing enriched mouse cells, identifying several unrecognized neurons. Many these displayed strong conservation across species, including...
Exposure to maternal obesity may promote metabolic dysfunction in offspring. We used infant mesenchymal stem cells (MSCs) experimentally examine cellular mechanisms of intergenerational health transmission. Our earlier reports show MSCs collected from infants mothers with had a dichotomous distribution efficiency; they were either efficient (Ef-Ob) or inefficient (In-Ob) respect fatty acid oxidation (FAO). Here, we sought determine if this was due primary defect FAO. Accordingly, measured...
Our objective was to interrogate mesenchymal stem cell (MSC) lipid metabolism and gestational exposures beyond maternal body mass that may contribute child obesity risk. MSCs were cultured from term infants of mothers with (n = 16) or normal weight 15). In undergoing myogenesis in vitro, we used lipidomics distinguish phenotypes by unbiased cluster analysis challenge (24-hour excess fatty acid [24hFA]). We measured MSC AMP-activated protein kinase (AMPK) activity oxidation (FAO), a composite...
Abstract Objective In human studies, new model systems are needed for improved mechanistic investigation of developmental predisposition metabolic disease but also to serve as benchmarks in early life prevention or intervention efforts. this regard, infant umbilical cord–derived mesenchymal stem cells (MSCs) an emerging tool. However, long‐term clinical relevance vivo markers is unknown. Methods a cohort 124 mother/child dyads, study tested the hypothesis that triglyceride content (TG) MSCs...
Abstract Objective Fat content of adipocytes derived from infant umbilical cord mesenchymal stem cells (MSCs) predicts adiposity in children through 4 to 6 years age. This study tested the hypothesis that MSCs infants born mothers with obesity (Ob‐MSCs) exhibit adipocyte hypertrophy and perturbations genes regulating adipogenesis compared normal weight (NW‐MSCs). Methods Adipogenesis was induced embedded three‐dimensional hydrogel structures, cell size number were measured by imaging....