A5067242440- Cannabis and Cannabinoid Research
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Sleep and Wakefulness Research
- Sleep and related disorders
- Nicotinic Acetylcholine Receptors Study
- Circadian rhythm and melatonin
- Neuroscience of respiration and sleep
- Forensic Toxicology and Drug Analysis
- Pharmacological Receptor Mechanisms and Effects
- Prenatal Substance Exposure Effects
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Memory and Neural Mechanisms
- Psychedelics and Drug Studies
- Tryptophan and brain disorders
- Pancreatic function and diabetes
- Alcohol Consumption and Health Effects
- Genetic Neurodegenerative Diseases
- Pain Mechanisms and Treatments
- Zebrafish Biomedical Research Applications
- HIV, Drug Use, Sexual Risk
- Cancer, Stress, Anesthesia, and Immune Response
- Diabetes Management and Research
- Phosphodiesterase function and regulation
Universidad Francisco de Vitoria
2017-2025
Universitat Pompeu Fabra
2010-2019
Neurocentre Magendie
2017
Inserm
2017
Barcelona Biomedical Research Park
2011
Universidad Complutense de Madrid
1997-2002
Cannabinoid CB1 receptors emerge early in the rat brain during prenatal development, supporting their potential participation events related to neural development. In present investigation, we completed earlier studies, analyzing receptor binding and mRNA expression by using autoradiography situ hybridization, respectively, of fetuses at gestational day (GD) 21 newborns postnatal days (PND) 1 5, comparison with adult brain. These analyses were paralleled quantitation levels anandamide its...
Endogenous cannabinoid receptor ligands (endocannabinoids) may rescue neurons from glutamate excitotoxicity. As these substances also accumulate in cultured immature following neuronal damage, elevated endocannabinoid concentrations be interpreted as a putative neuroprotective response. However, it is not known how glutamatergic insults affect vivo homeostasis, i.e. N ‐arachidonoylethanolamine (anandamide) and 2‐arachidonoylglycerol (2‐AG), well other constituents of their lipid families,...
The involvement of mu-opioid receptors in different behavioral responses elicited by nicotine was explored using receptor knock-out mice. acute antinociceptive induced the tail-immersion and hot-plate tests were reduced mutant mice, whereas no difference between genotypes observed locomotor responses. rewarding effects then investigated conditioning place-preference paradigm. Nicotine produced wild-type mice but failed to produce place preference indicating inability this drug induce absence...
Emerging evidence suggests that the hypocretinergic system is involved in addictive behavior. In this study, we investigated role of these hypothalamic neuropeptides anxiety-like responses nicotine and stress-induced reinstatement nicotine-seeking Acute (0.8 mg/kg, s.c.) induced anxiogenic-like effects elevated plus-maze activated paraventricular nucleus hypothalamus (PVN) as revealed by c-Fos expression. Pretreatment with hypocretin receptor 1 (Hcrtr-1) antagonist SB334867 or...
Abstract: Recent studies have shown that the pharmacological tolerance observed after prolonged exposure to synthetic or plant‐derived cannabinoids in adult rats is accompanied by down‐regulation/desensitization of brain cannabinoid receptors. However, no evidence exists on possible changes contents endogenous ligands receptors cannabinoid‐tolerant rats. The present study was designed elucidate this possibility measuring, means isotope dilution gas chromatography/mass spectrometry, both...
ABSTRACT In the present work, we analyzed cannabinoid receptor mRNA expression, binding and activation of signal transduction mechanisms in fetal rat brain or cultures neuronal glial cells. Cannabinoid expression were already measurable at GD14, but they only located discrete regions GD16. Among these, hippocampus, cerebellum caudate-putamen area, three that contain a marked for both adult brain. Significant levels and, particular, transcripts also detected GD16 cerebral cortex, midbrain...
Previous evidence suggests that the endogenous cannabinoid system could emerge and be operative early during brain development. In present study, we have explored distribution of specific binding for receptors in rat at gestational day 21 (GD21), postnatal days 5 (PND5) 30 (PND30), adult age (> 70 after birth) by using autoradiography with [3H]CP-55,940. Our results indicated can detected fetuses GD21 classic areas contain these adulthood-in particular, cerebellum hippocampus and, to a...
It has been shown previously that the endogenous opioid system may be involved in behavioral effects of nicotine. In present study, participation enkephalins on nicotine responses investigated by using preproenkephalin knock-out mice. Acute nicotine-induced hypolocomotion remained unaffected these contrast, antinociception elicited tail-immersion and hot-plate tests acute administration was reduced mutant animals. The rewarding properties were then place-conditioning paradigm. Nicotine...
Recent studies have demonstrated that the pharmacological tolerance observed after prolonged exposure to plant or synthetic cannabinoids in adult individuals seems a pharmacodynamic rather than pharmacokinetic basis, because down-regulation of cannabinoid receptors was assessed brain cannabinoid-tolerant rats. In present study, we examined time-course receptor by analyzing binding, using autoradiography, and mRNA expression, situ hybridization, several structures male rats daily exposed...
Abstract Recent studies have demonstrated a loss of cannabinoid CB 1 receptors in the postmortem basal ganglia patients affected by Huntington's disease (HD) and transgenic mouse models for this disease. These led to notion that substances increase endocannabinoid activity, such as receptor agonists or inhibitors uptake and/or metabolism, might be useful treatment hyperkinetic symptoms In present study, we employed rat model HD generated bilateral intrastriatal injections 3‐nitropropionic...