A5050402864- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- S100 Proteins and Annexins
- Hedgehog Signaling Pathway Studies
- Barrier Structure and Function Studies
- Protease and Inhibitor Mechanisms
- Acute Ischemic Stroke Management
- Pluripotent Stem Cells Research
- Hippo pathway signaling and YAP/TAZ
- Sinusitis and nasal conditions
- Neuroscience of respiration and sleep
- Congenital Diaphragmatic Hernia Studies
- Endoplasmic Reticulum Stress and Disease
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- Pediatric health and respiratory diseases
- Immunodeficiency and Autoimmune Disorders
- Cell Adhesion Molecules Research
- Bacterial Genetics and Biotechnology
- Child Nutrition and Feeding Issues
- Clinical practice guidelines implementation
- Obstructive Sleep Apnea Research
- Genetic and Kidney Cyst Diseases
- RNA modifications and cancer
Université de Reims Champagne-Ardenne
2011-2025
Inserm
2014-2025
Pathologies Pulmonaires et Plasticité Cellulaire
2019-2023
Centre Hospitalier Universitaire de Reims
2011-2023
Centre National de la Recherche Scientifique
2013
University of Liège
2011
Institute of Molecular and Cell Biology
2011
Abstract Chronic inflammation is a hallmark of cystic fibrosis ( CF ) lung disease and airway epithelium damage remodelling are important components pathology progression in . Whether this secondary to deleterious infectious inflammatory mediators, or alterations human epithelial HAE cells, such as their hyper phenotype basic transmembrane conductance regulator CFTR default, remains debated. In study, we evaluated the involvement cells remodelling. from non‐ patients were cultured an...
Background Cystic fibrosis (CF) is characterized by defective mucociliary clearance (MCC), chronic lung infection and exaggerated neutrophilic inflammation. Airway epithelium damage remodelling are important components of the pathology progression in CF affecting its defence functions. Identification compounds favouring MCC through an improvement airway epithelial structure regeneration therefore crucial for patients with (pwCF). Materials Methods Using air-liquid interface (ALI) culture...
Hedgehog (HH) pathway is constantly under scrutiny in the context of organ development. Lung morphogenesis requires HH signalling which participates thereafter to pulmonary homeostasis by regulating epithelial cell quiescence and repair. Since remodelling a hallmark Chronic Obstructive Pulmonary Disease (COPD), we investigated whether main molecular actors participate airway differentiation analysed their alterations COPD patients. Sonic (Shh) secretion was assessed ELISA (AEC) air-liquid...
Background The regulation of mucociliary clearance is a key part the defense mechanisms developed by airway epithelium. If high aggregate quality evidence shows clinical effectiveness nasal irrigation, there lack studies showing intrinsic role different irrigation solutions allowing such results. This study investigated impact with pH and ionic compositions, eg, normal saline, non-diluted seawater diluted seawater, on mucosa functional parameters. Methods For this randomized, controlled,...
Accumulating data now suggest that ZO-1, once delocalized from tight junctions, could be implicated in the regulation of tumor-promoting genes. Because their major implication different steps tumor progression, we investigated here influence ZO-1 on chemokines expression breast cancer cells. Using GeneArray analysis to compare chemokine mRNA cells transfected with a siRNA against identified CXCL-8IL-8 as potential target signaling, being strongly downregulated following transfection....
Cystic Fibrosis (CF) is present due to mutations in the Transmembrane Conductance Regulator (CFTR) gene, most frequent variant being p.phe508del. The CFTR protein a chloride (Cl-) channel which defective and almost absent of cell membranes when p.Phe508del mutation present. p.Phe508del-CFTR retained endoplasmic reticulum (ER) together with inflammation infection triggers Unfolded Protein Response (UPR). During UPR, Activating Transcription Factor 6 (ATF6) activated cleavage then decreases...
<b>Background:</b> Airway remodelling is a hallmark of COPD but its physiopathology remains unclear. Since Hedgehog (HH) pathway has been shown to regulate repair and regeneration in the mouse lung, we investigated role human airway epithelial cell differentiation context COPD. <b>Methods:</b> Sections from formalin-fixed paraffin embedded lung tissues non-COPD patients were stained characterize localization activation HH elements. Human primary cells obtained nasal polyps differentiated...
Abstract For the World Health Organization, carbapenem-resistant Pseudomonas aeruginos a is critical priority for which new antimicrobial drugs are needed. Consequently, understanding underlying mechanisms of resistant bacteria infection will enable identification therapeutic targets. Loss OprD porin main determinant resistance to last resort carbapenem antibiotics and has been described enhance fitness in vivo virulence. Transposon sequencing high-throughput technique that makes it possible...
The CF airway epithelium (AE) exhibits large areas of basal or goblet cell hyperplasia, ciliated absence epithelial desquamation, leading to impaired mucociliary clearance (MCC). Lung tissue inflammation leads AE inflammatory memory hyperplasia and increased height, defect is independent inflammation. Identification therapies favouring MCC through regeneration improvement crucial for patients. We therefore examined the influence an A-bC on human regeneration. Nasal cells from patients were...
<b>Background:</b> An alteration of primary cilia is observed in remodelled airways the context COPD. Since this organelle required to activate Hedgehog (HH) signalling pathway orchestrating cell fate during lung development, we explored HH activation human airway epithelial differentiation and adult lungs from COPD non-COPD patients. <b>Methods:</b> Human cells were cultured nasal polyps bronchial brushing air-liquid interface conditions reconstitute an epithelium representative airways....