A5070664424- Virus-based gene therapy research
- Computational Drug Discovery Methods
- Microbial Metabolism and Applications
- Pharmacological Effects of Natural Compounds
- Enzyme Structure and Function
- Enzyme-mediated dye degradation
- Microbial Metabolic Engineering and Bioproduction
- COVID-19 and healthcare impacts
- Advanced Proteomics Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Metal-Catalyzed Oxygenation Mechanisms
- Protein Structure and Dynamics
- Neuroendocrine Tumor Research Advances
- Biochemical and Structural Characterization
- Radiomics and Machine Learning in Medical Imaging
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Bacteriophages and microbial interactions
- Biotin and Related Studies
- Biochemical and Molecular Research
- Medical Imaging and Pathology Studies
- Monoclonal and Polyclonal Antibodies Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
The University of Texas MD Anderson Cancer Center
2022-2023
Indiana University of Pennsylvania
2019-2023
Johns Hopkins Medicine
2022
Mayo Clinic in Florida
2022
WinnMed
2022
Johns Hopkins University
2022
California Lutheran University
2021
Effective consolidative chemoradiation (CRT) regimens are lacking. In this phase 1 trial, we evaluated the safety and efficacy of nab-paclitaxel, capecitabine, radiation therapy after induction chemotherapy in patients with locally advanced borderline-resectable pancreatic cancer (LAPC BRPC). Also, a computed tomography (CT)-based biomarker response.Eligible had pathologically confirmed ductal adenocarcinoma, underwent tomography-imaging, received diagnosis LAPC or BRPC, chemotherapy....
Many industrial processes operate at elevated temperatures or within broad pH and salinity ranges. However, the utilization of enzymes to carry out biocatalysis in such is often impractical even impossible. Laccases (EC 1.10.3.2), which constitute a large family multicopper oxidases, have long been used setting. Although fungal laccases are many respects considered superior their bacterial counterparts, receiving greater attention recently. Albeit lower redox potential than laccases,...
<i>Streptomyces</i>, the most important group of industrial microorganisms, is harvested in liquid cultures for production two-thirds all clinically relevant secondary metabolites. It demonstrated here that growth <i>Streptomyces coelicolor</i> A3(2) impacted by deletion alanine dehydrogenase (ALD), an essential enzyme plays a central role carbon and nitrogen metabolism. A long lag-phase followed slow exponential <i>S. due to ALD gene was observed yeast extract...
Abstract Given the ever‐present threat of antibacterial resistance, there is an urgent need to identify new drugs and targets. One such target alanine racemase (Alr), enzyme required for bacterial cell‐wall biosynthesis. Alr attractive drug because it essential survival but absent in humans. Existing targeting lack specificity have severe side effects. We here investigate alternative mechanisms inhibition. functions exclusively as obligate homodimer, so we probed seven conserved interactions...
An understanding of structure-function relationships in proteins is essential for modern biochemical studies. The integration common freely accessible bioinformatics tools available online with the knowledge protein-engineering provide a fundamental application protein research. In order students to apply their prior recombinant technology into relationships, we developed semester-long project-oriented biochemistry laboratory experience that second course series. For easier and application,...
Abstract Given the ever-present threat of antibacterial resistance, there is an urgent need to identify new drugs and targets. One such target alanine racemase (Alr), enzyme required for bacterial cell-wall biosynthesis. Alr attractive drug because it essential survival but absent in humans. Here, we investigate from M. tuberculosis (MT), pathogen responsible human tuberculosis, as a model enzyme. MT-Alr functions exclusively obligate homodimer formed by two identical monomers. Both monomers...
Abstract Gastric cancer is the third leading cause of cancer-related mortality worldwide, and current standard-of-care treatment allows for a 5-year survival rate 20%. Newer treatments against solid tumors include an oncolytic adenovirus armed with T-cell activator OX40L, named Delta-24-RGDOX, which expresses positive immune checkpoint OX-40L. While studies have revealed interaction between gut microbiome immunotherapy agents, it’s still unknown how might influence viroimmunotherapy...
The surge in vancomycin-resistant enterococci (VRE) strains poses a serious threat to public and clinical health. VRE are the leading cause of multi-drug resistant enterococcal infections commonly acquired from medical devices. Therefore, it is essential discover new antibacterial targets drugs for this pathogen. Alanine racemase could be valuable drug target due its crucial role E. faecium survival. Alr (EF_Alr) was heterologously produced purified coli., steady-state kinetic constants were...