A5028942353- Autoimmune and Inflammatory Disorders Research
- Single-cell and spatial transcriptomics
- Systemic Lupus Erythematosus Research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immunodeficiency and Autoimmune Disorders
- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Spondyloarthritis Studies and Treatments
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Gene expression and cancer classification
- Inflammatory Bowel Disease
- Epilepsy research and treatment
- Heart Failure Treatment and Management
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Immune responses and vaccinations
- COVID-19 Impact on Reproduction
- Animal Virus Infections Studies
- Cardiac Fibrosis and Remodeling
SingHealth Duke-NUS Academic Medical Centre
2019-2024
Abstract Epileptogenic triggers are multifactorial and not well understood. Here we aimed to address the hypothesis that inappropriate pro-inflammatory mechanisms contribute pathogenesis of refractory epilepsy (non-responsiveness antiepileptic drugs) in human patients. We used single-cell cellular indexing transcriptomes epitopes by sequencing (CITE-seq) reveal immunotranscriptome surgically resected epileptic lesion tissues. Our approach uncovered a microenvironment, including extensive...
There is limited information on the functional neutralizing capabilities of breastmilk SARS-CoV-2-specific antibodies and potential adulteration with vaccine mRNA after SARS-CoV-2 vaccination. We conducted a prospective cohort study lactating healthcare workers who received BNT162b2 their infants. The presence antibodies, antibody isotypes (IgG, IgA, IgM) intact in serum was evaluated at multiple time points using surrogate assay, ELISA, PCR, over 6 week period two-dose vaccination given 21...
Drug refractory epilepsy (RE) is a chronic neurological disease with varied etiology that represents group of patients whose seizures do not respond to anti-epileptic drugs. The immune system may have role in seizure and development, but the specific mechanisms inflammation lead epileptogenesis contribute RE are unknown. Here, we used mass cytometry comprehensively study pediatric compared their profile function age-matched autoimmune encephalitis (AIE) healthy controls. Patients AIE...
Abstract Objectives Immunogenicity to the SARS-CoV-2 mRNA vaccines in adolescents and young adults (AYA) with childhood-onset rheumatic diseases (cRD) is unknown. We aimed evaluate humoral immunogenicity safety of our AYA cRD. Methods A monocentric observational study 159 (50.3% female 70.4% Chinese). Humoral was assessed at 2–3 4–6 weeks following first second vaccination by cPass™ Neutralization Antibody Assay. Inhibition signal ≥30% defined cut-off for positive detection neutralizing...
Chronic heart failure (HF) is a syndrome of heterogeneous etiology associated with multiple co-morbidities. Inflammation increasingly recognized as key contributor to the pathophysiology HF. Heterogeneity and lack data on immune mechanism(s) contributing HF may partially underlie clinical trials targeting inflammatory mediators. We studied Immunome in cohort using mass cytometry used data-driven systems immunology approach discover characterize modulated cell subsets from peripheral blood....
Background: We created a high dimensionality healthy human Immunome atlas by interrogating the peripheral blood mononuclear cells (PBMC) of >200 subjects (cord to adult) with 63 unique mechanistic and phenotypic markers per cell mass cytometry (CyTOF). This database is built an open source, web-based bioinformatics toolkit, enabling its mining uploading datasets for comparison EPIC database. Objectives: Here, we demonstrate platform’s ability identify immunological differences...
An accurate depiction of the convalescent COVID-19 immunome will help delineate immunological milieu crucial for disease resolution and protection. Using mass cytometry, we characterized immune architecture in patients recovering from mild COVID-19. We identified a virus-specific rheostat composed an effector T (T eff ) cell recall response that is balanced by enrichment highly specialized regulatory reg subset. Both components were reactive against peptide pool covering receptor binding...
To evaluate the humoral immunogenicity for 6 months after two-dose coronavirus disease 2019 (COVID-19) mRNA vaccination in adolescents and young adults (AYAs) with childhood-onset rheumatic diseases (cRDs).This monocentric observational study was conducted between August 2020 March 2022. Humoral assessed at 2-3 weeks first vaccine dose 1, 3 second by cPass™ severe acute respiratory syndrome 2 (SARS-CoV-2) neutralization antibody (nAb) assay. An inhibition signal of ≥30% defined...
<h3>Background:</h3> Circulatory CD4<sup>+</sup>HLADR<sup>+</sup> effector and regulatory T cells have been previously shown to possess strong overlap in immunological phenotypes TCRs with synovium counterparts[1,2]. In particular, systemic effectors were significantly increased arthritic patients that are non-responsive TNFi treatment. Yet, despite of the presence within synovium, why continue perpetuate inflammation its relationship counterparts remains unknown. <h3>Objectives:</h3> This...
<h3>Background</h3> Enthesitis-related arthritis (ERA), a common subtype of juvenile idiopathic (JIA), carries poor prognosis. Current therapies, including anti-TNF agents, are limited in controlling disease. There is understanding ERA immunopathogenesis, especially the ontogeny synovial pro-inflammatory T cells, which limits prognostic and therapeutic targets. <h3>Objectives</h3> We aim to compare, using high-dimensional approach, circulatory immune architectures active patients. Through...
<h3>Background</h3> We have previously described the presence of pathogenic-like lymphocyte (CPLs; CD4<sup>+</sup>HLA-DR<sup>+</sup>) subset within circulation, that is phenotypically similar to their synovial counterparts. CPLs are inflammatory T effectors correlate with disease activity and possess a TCR repertoire strong overlap. also discovered inflammation-associated T-regulatory (iaTreg; circulatory subset, activated, suppressive, poised migrate inflamed site sharing between peripheral...
<h3>Background</h3> Systemic lupus erythematosus (SLE) is a complex, systemic autoimmune disease. Disease presentation heterogeneous and characterized by alternating flares remissions. Recent treatment developments have been made with FDA approval of anifrolumab for adult SLE in 2021 <sup>[1]</sup> but there are many patients inadequate therapeutic response. Furthermore, lack specific molecular targets, current therapy relies mainly on general immunosuppression. This can adversely affect...
Background: Enthesitis-related arthritis (ERA) is one of the most common subtype juvenile idiopathic (JIA) in Asia 1 . It carries a poor prognosis, but limited knowledge its pathogenesis hampers clinical diagnosis and treatment. Objectives: We hypothesise multiple aberrations from healthy immunome culminating an imbalance between immune effector regulatory cell subsets as key for driving ERA pathogenesis. Thus, we employed comprehensive high-dimensional interrogative strategy using mass...