A5047929616- Autoimmune and Inflammatory Disorders Research
- Systemic Lupus Erythematosus Research
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Immunodeficiency and Autoimmune Disorders
- Respiratory Support and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Atherosclerosis and Cardiovascular Diseases
- T-cell and B-cell Immunology
- Neonatal Respiratory Health Research
- Spondyloarthritis Studies and Treatments
- COVID-19 Clinical Research Studies
- Complement system in diseases
- Phagocytosis and Immune Regulation
- Cytomegalovirus and herpesvirus research
- Systemic Sclerosis and Related Diseases
- Respiratory viral infections research
- Immune responses and vaccinations
- Pneumonia and Respiratory Infections
- Sepsis Diagnosis and Treatment
- Inflammatory Myopathies and Dermatomyositis
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
SingHealth
2017-2024
KK Women's and Children's Hospital
2015-2024
SingHealth Duke-NUS Academic Medical Centre
2018-2024
Duke-NUS Medical School
2017-2024
National University of Singapore
2011-2024
National Taiwan University Hospital
2023
Taipei Medical University
2023
University of Toledo
2018
Boston Children's Hospital
2011
Abstract Epileptogenic triggers are multifactorial and not well understood. Here we aimed to address the hypothesis that inappropriate pro-inflammatory mechanisms contribute pathogenesis of refractory epilepsy (non-responsiveness antiepileptic drugs) in human patients. We used single-cell cellular indexing transcriptomes epitopes by sequencing (CITE-seq) reveal immunotranscriptome surgically resected epileptic lesion tissues. Our approach uncovered a microenvironment, including extensive...
Gut microbiota are associated with a variety of complex polygenic diseases. The usage broad-spectrum antibiotics by patients affected such diseases is an important environmental factor to consider, because antibiotics, which widely prescribed curb pathological bacterial infections, also indiscriminately eliminate gut commensal microbiota. However, the extent reshape and per se contribute these understudied. Because genetics play role in predisposing individuals modern diseases, we...
Objective evaluation of disease activity is challenging in patients with juvenile dermatomyositis ( DM ) due to a lack reliable biomarkers, but it crucial avoid both under‐ and overtreatment patients. Recently, we identified 2 proteins, galectin‐9 CXCL 10, whose levels are highly correlated the extent activity. This study was undertaken validate 10 as biomarkers for , assess their specificity potency predicting occurrence flares. Methods Levels were measured by multiplex immunoassay serum...
There is limited information on the functional neutralizing capabilities of breastmilk SARS-CoV-2-specific antibodies and potential adulteration with vaccine mRNA after SARS-CoV-2 vaccination. We conducted a prospective cohort study lactating healthcare workers who received BNT162b2 their infants. The presence antibodies, antibody isotypes (IgG, IgA, IgM) intact in serum was evaluated at multiple time points using surrogate assay, ELISA, PCR, over 6 week period two-dose vaccination given 21...
Acute respiratory distress syndrome (ARDS) represents the most severe form of acute lung injury. The aim our study is to describe epidemiology pediatric ARDS in Singapore and compare outcomes using following indices: PaO2/FiO2 ratio (P/F ratio), SpO2/FiO2 (S/F oxygenation index (OI), oxygen saturation (OSI).We examined medical records patients admitted Children's Intensive Care Unit KK Women's Hospital from 2009 2012. Those who fulfilled criteria for American-European Consensus Conference...
Drug refractory epilepsy (RE) is a chronic neurological disease with varied etiology that represents group of patients whose seizures do not respond to anti-epileptic drugs. The immune system may have role in seizure and development, but the specific mechanisms inflammation lead epileptogenesis contribute RE are unknown. Here, we used mass cytometry comprehensively study pediatric compared their profile function age-matched autoimmune encephalitis (AIE) healthy controls. Patients AIE...
Juvenile dermatomyositis (DM) is a heterogeneous systemic immune-mediated vasculopathy. This study was undertaken to 1) identify inflammation/endothelial dysfunction-related biomarker profiles reflecting disease severity at diagnosis, and 2) establish whether such could be used for predicting the response treatment in patients with juvenile DM.In total, 39 biomarkers related activation of endothelial cells, dysfunction, inflammation were measured using multiplex technology serum samples from...
Effective clearance of apoptotic cells by phagocytes prevents the release intracellular alarmins and manifestation autoimmunity. This prompt efferocytosis is complemented proteolytic degradation that occurs within in efferosome phagocytes. Although role extracellular proteases unknown, strong association congenital C1s deficiency with Systemic Lupus Erythematosus highlights protective nature this protease has against The archetypical serine as catalytic arm C1 complex (C1qC1r2C1s2) involve...
Regulatory T cells (Tregs) are often enriched in tumors, where their immunosuppressive function has a key role tumor persistence and progression. In colorectal cancer (CRC), however, Tregs frequently associated with an improved clinical outcome. Tumor-infiltrating have been shown to exhibit distinct signature comprising the co-stimulatory molecules (OX40, 4-1BB), cytokine receptors (IL1R2, IL21R, CCR8, CD30), co-inhibitory (PD-L1, TIGIT). Here, we showed by flow cytometry that circulating...
Abstract Objectives Immunogenicity to the SARS-CoV-2 mRNA vaccines in adolescents and young adults (AYA) with childhood-onset rheumatic diseases (cRD) is unknown. We aimed evaluate humoral immunogenicity safety of our AYA cRD. Methods A monocentric observational study 159 (50.3% female 70.4% Chinese). Humoral was assessed at 2–3 4–6 weeks following first second vaccination by cPass™ Neutralization Antibody Assay. Inhibition signal ≥30% defined cut-off for positive detection neutralizing...
Abstract Objectives Vasculopathy is an important hallmark of systemic chronic inflammatory connective tissue diseases (CICTD) and associated with increased cardiovascular risk. We investigated disease-specific biomarker profiles endothelial dysfunction, angiogenic homeostasis (tissue) inflammation, their relation to disease activity in rare CICTD. Methods A total 38 serum proteins (dys)function inflammation were measured by multiplex-immunoassay treatment-naive patients localized scleroderma...
Objectives Biologics treatment with antitumour necrosis factor alpha (TNFα) is efficacious in patients juvenile idiopathic arthritis (JIA). Despite displaying clinical inactivity during treatment, many will flare on cessation of therapy. The inability to definitively discriminate who relapse or continue remain remission after therapy withdrawal currently a major unmet medical need. CD4 T cells have been implicated active disease, yet how they contribute disease persistence despite unknown....
Chronic heart failure (HF) is a syndrome of heterogeneous etiology associated with multiple co-morbidities. Inflammation increasingly recognized as key contributor to the pathophysiology HF. Heterogeneity and lack data on immune mechanism(s) contributing HF may partially underlie clinical trials targeting inflammatory mediators. We studied Immunome in cohort using mass cytometry used data-driven systems immunology approach discover characterize modulated cell subsets from peripheral blood....
This study aimed to determine the usefulness and validity of triaging scale used in our emergency department (ED) by analysing its association with surrogate clinical outcome measures severity consisting hospitalisation rate, intensive care unit (ICU) admission, length ED stay, predictive value for admission hospitalisation.A retrospective observational was conducted performance markers Singapore Paediatric Triage Scale (SPTS) identify children who needed immediate greater care. All triaged...
Summary Background and Objective Bronchiolitis is a common disease in early childhood with increasing healthcare utilization. We aim to study how well simple improved respiratory score (the modified Respiratory Index Score [RIS]) would perform when predicting for warranted admission. Methods This an observational prospective study, from June 2015 December paediatric emergency department (ED) of large tertiary hospital Singapore. included children aged less than 2 years old, presenting...