A5015074866- Bone Metabolism and Diseases
- Intracranial Aneurysms: Treatment and Complications
- Rheumatoid Arthritis Research and Therapies
- Foot and Ankle Surgery
- Bone health and treatments
- Orthopedic Surgery and Rehabilitation
- Traumatic Brain Injury and Neurovascular Disturbances
- Vascular Malformations Diagnosis and Treatment
- Neurosurgical Procedures and Complications
- Moyamoya disease diagnosis and treatment
- TGF-β signaling in diseases
- Lower Extremity Biomechanics and Pathologies
- Intracerebral and Subarachnoid Hemorrhage Research
- Bone and Joint Diseases
- Tendon Structure and Treatment
- Cerebrovascular and Carotid Artery Diseases
- Diabetic Foot Ulcer Assessment and Management
- NF-κB Signaling Pathways
- Musculoskeletal synovial abnormalities and treatments
- Osteoarthritis Treatment and Mechanisms
- Advanced MRI Techniques and Applications
- Autoimmune and Inflammatory Disorders Research
- Cerebrospinal fluid and hydrocephalus
- Total Knee Arthroplasty Outcomes
- MicroRNA in disease regulation
Teikyo University
2015-2024
High Energy Accelerator Research Organization
2024
Japan Proton Accelerator Research Complex
2024
The University of Tokyo
2010-2021
University of Tokyo Hospital
2006-2020
Teikyo University Hospital
2017-2019
Hokkaido University
2015
Nippon Medical School
2015
Jikei University School of Medicine
2015
Kyorin University
2015
Abstract Gene expression is controlled by epigenetic mechanisms such as histone acetylation and methylation, recent studies have revealed that key developmental steps are regulated the trimethylation of H3 lysine 4 (H3K4me3) 27 (H3K27me3). Using ChIP sequencing technology combined with real-time PCR, we here demonstrate H3K27me3 observed in Nfatc1 gene bone marrow–derived macrophages (BMMs) was markedly reduced mature osteoclasts. Jumonji domain-containing 3 (Jmjd3), a H3K27 demethylase,...
I " IA cDNA clone for the nitratelnitrite-inducible cytochrome P-450 (P-450) of fungus Fusarium oxysporum (tentatively termed P'450dNIR) was isolated by an immunoscreening method.Sequence determination revealed a polypeptide 403 amino acid residues (Mr = 44,371), which shown to contain full-length sequence fungal P-450.The terminus region predicted contained neither signal-like, hydrophobic domain that is commonly observed in microsomal P-450s nor tagging prosequence essential localization...
Abstract Previous studies have shown that transforming growth factor β (TGF-β) promotes receptor activator of nuclear factor-κB ligand (RANKL)–induced osteoclastogenesis. However, the underlying molecular mechanisms not been elucidated. When TGF-β signals were blocked either by a specific inhibitor type 1 kinase activity, SB431542, or introducing dominant-negative mutant 2 receptor, RANKL-induced osteoclastogenesis was almost completely suppressed. Blockade Smad signaling overexpression...
Abstract Rheumatoid arthritis (RA) is an autoimmune destructive associated with CD4 + T cell-mediated immunity. Although expanded cell clones (ECs) has already been confirmed, the detailed characteristics of ECs have not elucidated in RA. Using combination a single-cell analysis and next-generation sequencing (NGS) TCR repertoire analysis, we here revealed nature by examining peripheral blood (PB) from 5 RA patients synovium 1 patient. When intensively investigated transcriptome most memory...
<h3>Objectives:</h3> The aim of this study was to identify risk factors for rupture unruptured cerebral aneurysms (UCAs) in elderly Japanese patients aged 70 years or older. <h3>Methods:</h3> participants included all age older 3 prospective studies Japan (the Unruptured Cerebral Aneurysm Study [UCAS Japan], UCAS II, and the at Jikei University School Medicine). A total 1,896 with 2,227 UCAs were investigated. median mean follow-up periods 990 802.7 days, respectively. <h3>Results:</h3>...
The anti-apoptotic molecule Bcl-2 inhibits apoptosis by preventing cytochrome c release from mitochondria. Although several studies have indicated the importance of in maintaining skeletal integrity, detailed cellular and molecular mechanisms remain elusive. Bcl-2−/− mice are growth-retarded exhibit increased bone volume primary spongiosa, mainly due to decreased number dysfunction osteoclasts. Osteoblast function is also impaired mice. Ex vivo on osteoblasts osteoclasts showed that promoted...
ABSTRACT Receptor activator of nuclear factor κB ligand (RANKL) induces osteoclast (OC) differentiation from bone marrow-derived macrophages (BMMs). The transcription factors activated T cells 1 (NFATc1) and interferon regulatory (IRF) 8 play positive negative roles, respectively, in this process. However, genomewide mapping the active cis-regulatory elements regulating OC has not been performed, little is known about global landscape OC-specific gene regulation. We used chromatin...
The B cell lymphoma 2 (Bcl-2) family member Bcl-xL has a well-characterized antiapoptotic function in lymphoid cells. However, its functions other cells — including osteoclasts, which are of hematopoietic origin and cellular processes remain unknown. Here we report an unexpected attenuating the bone-resorbing activity osteoclasts mice. To investigate role generated mice with osteoclast-specific conditional deletion Bcl-x (referred to herein as cKO mice) by mating Bcl-xfl/fl gene encoding Cre...
Abstract We investigated the role of protein kinase B (Akt), a downstream effector phosphatidylinositol 3-kinase, in bone-resorbing activity mature osteoclasts. Treatment with specific Akt inhibitor disrupted sealing zone formation and decreased The normal microtubule structures were lost reduced amount acetylated tubulin, which reflects stabilized microtubules, whereas forced activation by adenovirus vectors resulted opposite effect. Forced increased binding microtubule-associated...
We have previously reported that transforming growth factor β (TGF-β) plays an essential role in receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis. However, the detailed underlying molecular mechanisms still remain unclear. Formaldehyde-assisted isolation regulatory elements (FAIRE) and chromatin immunoprecipitation (ChIP) followed by sequencing (FAIRE-seq ChIP-seq) analyses indicated cooperation Smad2/3 with c-Fos during Biochemical analysis...
Total ankle arthroplasty (TAA) has become increasingly popular worldwide as an alternative to arthrodesis for surgical treatment of end-stage arthritis. The aim this epidemiological study, using a national inpatient database in Japan, was describe the volume, utilization, patient characteristics, and temporal trends regarding these procedures identify risk factors associated with perioperative adverse events TAA. This population-based, retrospective cohort study. We retrospectively...
Signal transducer and activator of transcription 5 (Stat5) is essential for cytokine-regulated processes such as proliferation, differentiation, survival in hematopoietic cells. To investigate the role Stat5 osteoclasts, we generated mice with an osteoclast-specific conditional deletion (Stat5 knockout [cKO] mice) analyzed their bone phenotype. cKO exhibited osteoporosis caused by increased bone-resorbing activity osteoclasts. The mitogen-activated protein kinases (MAPKs), particular...
We examined the individual and combined effects of teriparatide anti-RANKL (receptor activator nuclear factor κB ligand) monoclonal antibody in ovariectomized mice. Three-month-old female C57BL/6 mice were (OVX) or sham operated. Four weeks after OVX, they assigned to 3 different groups receive (Ab) alone (5 mg/kg single injection at 4 Ab group), (80 μg/kg daily for from PTH mAb plus (Ab + group). Mice sacrificed 8 OVX. Bone mineral density (BMD) was measured femur lumbar spine. Hind limbs...
Assessing the effect of insoles on gait biomechanics and foot comfort remains challenging. Our novel in-insole-type wearable sensor device (smart insole) enables accurate quantitative evaluation parameters without affecting subject's comfort.
Nitrogen-containing bisphosphonates are one of the most successful therapeutics for osteoporosis. The aim this study was to elucidate functional mechanism typical nitrogen-containing bisphosphonates, risedronate.Osteoclasts generated from murine bone marrow macrophages were treated with risedronate in vitro, and its effects on apoptosis bone-resorbing activity examined. action examined by gene induction constitutively active Akt-1 MEK-1, deletion Bim. Bim(-/-) mice, which osteoclasts...