A5055706716- Bone Metabolism and Diseases
- Gallbladder and Bile Duct Disorders
- Cytokine Signaling Pathways and Interactions
- Pancreatic and Hepatic Oncology Research
- Cardiac Arrhythmias and Treatments
- Esophageal and GI Pathology
- Single-cell and spatial transcriptomics
- Cardiac electrophysiology and arrhythmias
- Inflammatory mediators and NSAID effects
- Mesenchymal stem cell research
- Galaxies: Formation, Evolution, Phenomena
- Pediatric Hepatobiliary Diseases and Treatments
- Immune cells in cancer
- RNA Research and Splicing
- Chemokine receptors and signaling
- Pluripotent Stem Cells Research
- Heterotopic Ossification and Related Conditions
- Osteoarthritis Treatment and Mechanisms
- Hip and Femur Fractures
- Multiple Myeloma Research and Treatments
- Signaling Pathways in Disease
- Stellar, planetary, and galactic studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- NF-κB Signaling Pathways
- Oral microbiology and periodontitis research
The University of Tokyo
2014-2025
Harvard University
2024
University of Tokyo Hospital
1996-2022
Okayama University
2015-2021
Okayama University Hospital
2019-2021
Kobe Asahi Hospital
2020
BioScience Laboratories (United States)
2019
Musashino Red Cross Hospital
2011-2012
Osaka University
2006-2009
National Institute of Infectious Diseases
2009
We measure the redshift-space correlation function from a spectroscopic sample of 2783 emission line galaxies FastSound survey. The survey, which uses Subaru Telescope and covers redshift ranges $1.19<z<1.55$, is first cosmological study at such high redshifts. detect clear anisotropy due to distortions (RSD) both in as separations parallel perpendicular sight its quadrupole moment. RSD has been extensively used test general relativity on scales $z<1$. Adopting LCDM cosmology with fixed...
Osteoarthritis (OA) shows various clinical manifestations depending on the status of its joint components. We aimed to identify synovial cell subsets responsible for OA pathophysiology by comprehensive analyses human synovium samples in single-cell resolution. Two distinct tissue groups were classified gene expression profiles RNA-Seq: inflammatory and fibrotic. The group exhibited high cytokines, histologically infiltrate, a more severe pain score. fibrotic showed higher fibroblast growth...
Abstract The Runt-related transcription factor (Runx) family plays various roles in the homeostasis of cartilage. Here, we examined role Runx2 and Runx3 for osteoarthritis development vivo vitro. Runx3- knockout mice exhibited accelerated following surgical induction, accompanied by decreased expression lubricin aggrecan. Meanwhile, conditional showed biphasic phenotypes: heterozygous inhibited matrix metallopeptidase 13 (Mmp13) expression, while homozygous reduced type II collagen (Col2a1)...
The transcriptional regulator Runx2 (runt-related transcription factor 2) has essential but distinct roles in osteoblasts and chondrocytes skeletal development. However, Runx2-mediated regulatory mechanisms underlying the distinctive programming of are not well understood. Here, we perform an integrative analysis to investigate Runx2-DNA binding chromatin accessibility ex vivo using neonatal chondrocytes. We find that engages with cell-type-distinct chromatin-accessible regions, potentially...
Janus kinase (JAK) inhibitors, such as baricitinib, are widely used to treat rheumatoid arthritis (RA). Clinical studies show that baricitinib is more effective at reducing pain than other similar drugs. Here, we aimed elucidate the molecular mechanisms underlying relief conferred by using a mouse model of arthritis.
To understand the roles of phosphoinositides [PtdIns] in phagocytosis parasitic eukaryotes, we examined interaction phosphatidylinositol-3-phosphate [PtdIns(3)P] and putative PtdIns-P-binding proteins during enteric protozoan parasite Entamoeba histolytica. It was previously shown that E. histolytica is indispensable for virulence inhibited by PtdIns 3-kinase inhibitors. We demonstrated time-lapse live imaging initiation phagocytosis, PtdIns(3)P biomarker GFP-Hrs-FYVE, translocated to...
Ectopic endochondral ossification in the tendon/ligament is caused by repetitive mechanical overload or inflammation. Tendon stem/progenitor cells (TSPCs) contribute to tissue repair, and some express lubricin [proteoglycan 4 (PRG4)]. However, mechanisms of ectopic association TSPCs are not yet known. Here, we investigated characteristics Prg4-positive ( + ) identified that R-spondin 2 (RSPO2), a WNT activator, specifically expressed distinct Prg4 TSPC cluster. The Rspo2 cluster was...
Although the skeleton is essential for locomotion, endocrine functions, and hematopoiesis, molecular mechanisms of human skeletal development remain to be elucidated. Here, we introduce an integrative method model by combining in vitro sclerotome induction from pluripotent stem cells vivo endochondral bone formation implanting beneath renal capsules immunodeficient mice. Histological scRNA-seq analyses reveal that induced bones recapitulate ossification are composed mouse circulatory cells....
Runx2 is a master regulator of bone formation, and its dysfunction causes cleidocranial dysplasia (CCD) in humans. When iPS cells were generated from patients with CCD Runx2-deficient using gene-editing techniques, abnormal laminopathy-like nuclei observed. showed reduced Lamin A/C expression, but not protein levels. However, cells, both the gene expression levels Nesprin1 reduced, perinuclear actin fibers sparser, nuclear stiffness was reduced. Forced increased did improve morphology. In...
ABSTRACT Receptor activator of nuclear factor κB ligand (RANKL) induces osteoclast (OC) differentiation from bone marrow-derived macrophages (BMMs). The transcription factors activated T cells 1 (NFATc1) and interferon regulatory (IRF) 8 play positive negative roles, respectively, in this process. However, genomewide mapping the active cis-regulatory elements regulating OC has not been performed, little is known about global landscape OC-specific gene regulation. We used chromatin...
Abstract Vitamin D deficiency is a recognized risk factor for sarcopenia development, but mechanisms underlying this outcome are unclear. Here, we show that low vitamin status worsens immobilization-induced muscle atrophy in mice. Mice globally lacking receptor (VDR) exhibited more severe following limb immobilization than controls. Moreover, was worse neural crest-specific skeletal muscle-specific VDR-deficient Tnfα expression significantly higher immobilized of relative to control mice,...
Bone turnover is finely tuned by cells in the bone milieu, including osteoblasts, osteoclasts, and osteocytes. Osteoclasts are multinucleated giant with a bone-resorbing function that play critical role regulating skeletal homeostasis. Osteoclast differentiation characterized dramatic changes morphology gene expression following receptor activator of nuclear factor-kappa-Β ligand (RANKL) stimulation. We performed single-cell RNA-sequencing analyses human murine osteoclast-lineage (OLCs)...
We present the results from a large near-infrared spectroscopic survey with Subaru/FMOS (\textit{FastSound}) consisting of $\sim$ 4,000 galaxies at $z\sim1.4$ significant H$\alpha$ detection. measure gas-phase metallicity [N~{\sc ii}]$\lambda$6583/H$\alpha$ emission line ratio composite spectra in various stellar mass and star-formation rate bins. The resulting mass-metallicity relation generally agrees previous studies obtained similar redshift range to that our sample. No clear dependence...
Abstract Although multiple studies have investigated the mesenchymal stem and progenitor cells (MSCs) that give rise to mature bone marrow, high heterogeneity in their morphologies properties causes difficulties molecular separation of distinct populations. In this study, by taking advantage resolution single cell transcriptome, we analyzed Sca-1 PDGFR-α fraction mouse marrow tissue. The transcriptome enabled us further classify population into seven populations according gene expression...
ABSTRACT CTLA4-Ig (cytotoxic T-lymphocyte antigen 4-immunoglobulin; Abatacept) is a biologic drug for rheumatoid arthritis. CTLA4 binds to the CD80/86 complex of antigen-presenting cells and blocks activation T cells. Although previous reports showed that directly inhibited osteoclast differentiation, whole inhibitory mechanism differentiation unclear. Bone marrow macrophages (BMMs) from WT mice were cultured with M-CSF RANKL or without recombinant mouse chimera CTLA4-Ig. Intracellular...
FastSound is a galaxy redshift survey using the near-infrared Fiber Multi-Object Spectrograph (FMOS) mounted on Subaru Telescope, targeting H$α$ emitters at $z \sim 1.18$--$1.54$ down to sensitivity limit of flux $\sim 2 \times 10^{-16} \ \rm erg cm^{-2} s^{-1}$. The primary goal detect space distortions (RSD), test General Relativity by measuring growth rate large scale structure and constrain modified gravity models for origin accelerated expansion universe. target galaxies were selected...
Abstract Background and study aims We designed a new 7-Fr plastic stent for treating self-expandable metal (SEMS) obstruction in patients with malignant hilar biliary (MHBO) via endoscopic re-intervention the stent-in-stent (SIS) method evaluated its efficacy. Patients methods A total of 33 consecutive who underwent after multi-branched SEMS placement were enrolled. The initial SEMSs placed two or three branches 14 19 patients, respectively. retrospectively technical clinical success,...