A5019277382- Bacterial Genetics and Biotechnology
- DNA Repair Mechanisms
- Enzyme Structure and Function
- Protist diversity and phylogeny
- Microbial Natural Products and Biosynthesis
- Glutathione Transferases and Polymorphisms
- Neurological diseases and metabolism
- Acute Lymphoblastic Leukemia research
- Sulfur Compounds in Biology
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Reflective Practices in Education
- Statistics Education and Methodologies
- Pharmacogenetics and Drug Metabolism
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
- Genetic factors in colorectal cancer
- Mentoring and Academic Development
- Teacher Education and Leadership Studies
- Microbial Metabolism and Applications
- Higher Education Practises and Engagement
- Education and Technology Integration
- Phytoestrogen effects and research
- Advanced X-ray Imaging Techniques
University of Maryland, College Park
2011
Centre National de la Recherche Scientifique
2005
Institut de Biologie Physico-Chimique
2005
American Association For The Advancement of Science
2005
Université Paris Cité
2005
Columbia University
2002-2004
Johns Hopkins University
1996-2000
Johns Hopkins Medicine
1999
University of Maryland, Baltimore
1996
MutY is an adenine-DNA glycosylase with specificity for mismatches involving 8-oxoguanine (oG.A) or guanine (G.A). In addition to a 25 kDa catalytic domain common all members of its DNA superfamily, has 14 C-terminal domain. Sequence analyses suggest that this distantly related MutT, pyrophosphohydrolase specific 2'-deoxy-8-oxoguanosine triphosphate (doGTP). Here we present biochemical evidence the MutT-like principal determinant oG specificity. First, dissociates approximately 1500-fold...
Summary Operons encoding stable toxins and their labile antidote are widespread in prokaryotes play important roles plasmid partitioning cellular responses to stress. One such family of MazF/ChpAK/PemK encodes an endoribonuclease that inactivates mRNAs by cleaving them at specific, but frequently occurring sites. Here we show the Bacillus subtilis ydcE gene a member this RNases, which have called EndoA. Overexpression EndoA is toxic for bacterial cell growth toxicity reversed coexpression...
Escherichia coli MutY is a 39 kDa adenine DNA glycosylase and 3' apurinic/apyrimidinic (AP) lyase that active on substrates containing A/G, A/C, or A/8-oxoG mismatches. 8-oxoG (7,8-dihydro-8-oxoguanine GO) major stable product of oxidative damage, A/GO mismatches may be particularly important biological for MutY. Proteolytic digestion using thermolysin was found to produce two relatively fragments 25 12 kDa. The fragment begins at the N terminus spans region homologous with E. endonuclease...
Addiction modules, which consist of two genes encoding a toxin and an antitoxin, mediate plasmid maintenance in Escherichia coli by selectively killing plasmid-free cells (postsegregational killing).1 They are most often encoded on low–copy number plasmids. The operons controlling these modules autoregulated at the transcription level their corresponding toxin–antitoxin complex. Maintenance this complex prevents lethal effect toxin. Toxin activation is result higher decay rates for...
A thermostable 8-oxoguanine (oxoG) DNA glycosylase from Methanococcus jannaschii has been expressed in Escherichia coli, purified, and characterized. The enzyme, which named mjOgg, belongs to the same diverse superfamily as glycosylases yeast (yOgg1) human (hOgg1) but is substantially different sequence. In addition, unlike its eukaryotic counterparts, have a strong preference for oxoG.C base pairs, mjOgg little specificity opposite oxoG. both lyase (beta-elimination) activity, combined...
The Professional Science Master’s Biotechnology program at the University of Maryland College implemented a novel online mentoring that pairs masters’ students with mentors from biotechnology industry. has grown 19 mentor-mentee in fall 2009 to 46 2011. student retention is currently 80%. Data indicate who continued participating after first semester had completed more courses before joining than dropped. end-of questionnaires show general satisfaction and provide specific examples gains...
We have studied the relationship between amino acid sequence and substrate specificity in a DNA glycosylase family by characterizing experimentally of four new members family. show that principal component analysis (PCA) correctly predicts one novel homologs even though conventional methods fail to group this homolog with other sequences same specificity. PCA also suggested, correctly, another characterized previously differs its from those which it clusters criteria. These results suggest...
Crystal structures are reported for free and coenzyme A (CoA) bound forms of the YfdW protein from Escherichia coli, a representative type III CoA transferase. The reveal two-domain protomer with interdomain connections forming ring-like structure large central hole. Two protomers associate to form highly intertwined dimer in which hole each ring is filled by partner molecule. Each binds single molecule these CoA-binding sites distant one another dimer.
The University of Maryland Collegeâ??s (UMUC) Department Education developed a program to increase achievement secondary school students and retention Master Arts in Teaching Program (MAT) pre-service teacher candidates entitled, â??Professional Partnerships