A5020625925- Autophagy in Disease and Therapy
- Vibrio bacteria research studies
- Ubiquitin and proteasome pathways
- Animal Disease Management and Epidemiology
- Salmonella and Campylobacter epidemiology
- Vector-Borne Animal Diseases
- Cellular Mechanics and Interactions
- Hepatitis B Virus Studies
- Click Chemistry and Applications
- RNA modifications and cancer
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Virus Infections Studies
- interferon and immune responses
- Aquaculture disease management and microbiota
- Photochromic and Fluorescence Chemistry
- Immunotherapy and Immune Responses
- bioluminescence and chemiluminescence research
- HIV Research and Treatment
- Synthesis and Characterization of Heterocyclic Compounds
- Neuroscience and Neural Engineering
- Biotin and Related Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
Chinese Academy of Agricultural Sciences
2024
Institute of Animal Sciences
2024
Beijing University of Agriculture
2024
ShanghaiTech University
2018-2024
Center for Excellence in Molecular Cell Science
2020
Chinese Academy of Sciences
2020
Soochow University
2010-2016
Macroautophagy/autophagy is a membrane-mediated intracellular degradation pathway, through which bulky cytoplasmic content digested in lysosomes. How the autophagy initiation and maturation steps are regulated not clear. In this study, we found an E3 ubiquitin ligase complex, linear chain assembly complex (LUBAC) deubiquitinating enzyme (DUB) OTULIN localize to phagophore area control maturation. LUBAC key component RNF31/HOIP translocates LC3 puncta when induced. RNF31 knockdown inhibits...
Whether, to what extent, and how the axons in central nervous system (CNS) can withstand sudden mechanical impacts remain unclear. By using a microfluidic device apply controlled transverse stress axons, we determined levels that most explored their instant responses at nanoscale resolution. We found mild triggers highly reversible, rapid axon beading response, driven by actomyosin-II-dependent dynamic diameter modulations. This mechanism contributes hindering long-range spread of...
// Yuanyuan Chu 1 , Song Gao Ting Wang Jing Yan Guangmei Xu Li Hua Niu Rui Huang Shuyan Wu Department of Microbiology, Medical College Soochow University, Suzhou, P. R. China Correspondence to: Huang, e-mail: hruisdm@163.com Wu, wushuyan@suda.edu.cn Keywords: Salmonella spvB, autophagy, autophagic flux Received: July 03, 2015 Accepted: January 13, 2016 Published: 22, 2016 ABSTRACT plasmid virulence genes ( spv ) are highly conserved in strains...
African swine fever (ASF) is a highly contagious and lethal disease of caused by virus (ASFV), the mortality rate virulent stains can approach 100%. Many ASFV viral proteins suppress interferon production to evade host's innate immune responses. However, whether MGF360-4L could inhibit type I (IFN-I) signaling pathway underlying molecular mechanisms remain unknown. Our study, indicated that negatively regulates cGAS-STING mediated IFN-I pathway. Overexpressing cGAS/STING inhibiting...
AMFR/gp78 and USP13 are a pair of ubiquitin ligase deubiquitinase that ensure the accuracy endoplasmic reticulum-associated degradation (ERAD). Depletion leads to caspase activation cleavage ERAD chaperone BAG6, which is reversed by knockdown AMFR. However, mechanism physiological relevance this regulation still unclear. Here, using NEDDylator system, we screened out TXN as substrate AMFR showed its involvement in regulating CASP3 BAG6 cleavage. Furthermore, cleaved N-terminal located...
The African swine fever virus (ASFV) encodes numerous proteins characterized by complex immune escape mechanisms. At present, the structure and function of these proteins, including F317L protein, have yet to be fully elucidated. In this study, we examined immunogenicity protein. Mice were subcutaneously immunized with protein using initial subsequent booster doses, and, at 28th day post-treatment, assessed humoral cellular responses mice. stimulated production specific antibodies activated...
The highly conserved C129R protein of AFSV was utilized in the development an ASFV recombinant adenovirus vaccine, demonstrating strong immunogenicity. In this study, we immunized 6-week-old female C57BL/6J mice via subcutaneous injection with 10 μg purified protein. Humoral and cellular immune effects were assessed using ELISA, flow cytometry, ELISpot assays. Additionally, 19 peptides synthesized screened for use bioinformatics. Positive T-cell epitopes ELISpot. results indicated a higher...
Plasmid pRST98 is a hybrid resistance-virulence plasmid isolated from Salmonella enterica serovar Typhi (S. typhi). Previous studies demonstrated that could enhance the virulence of its host bacteria. However, mechanism pRST98-increased bacterial still not fully elucidated. This study was designed to gain further insight into roles in responses. Human-derived macrophage-like cell line THP-1 infected with wild-type (ST8), pRST98-deletion (ST8-ΔpRST98), and complemented (ST8-c-pRST98) S. typhi...
Salmonella evades host immune response via the expression of a variety pathogenic factors. The 'pRST98' plasmid enterica serotype Typhi (S. Typhi) is involved in conferring multidrug‑resistance and virulence S. typhi. However, its specific effect on host‑cell function has remained elusive. Dendritic cells (DCs) are key regulators responses. present study therefore aimed to investigate whether pRST98 may target DCs mediating adaptive response. In vivo experiments with Typhimurium χ3337...
Elucidating the subcellular localization of RNAs and proteins is fundamental to understanding their biological functions. Genetically encoded proteins/enzymes provide an attractive approach target many interest, but are limited specific cell lines. Although small‐molecule‐based methods have been explored, a comprehensive system for profiling multiple locations in living cells, comparable fusion‐protein techniques, yet be established. In this study, we introduce novel proximity labeling...
Elucidating the subcellular localization of RNAs and proteins is fundamental to understanding their biological functions. Genetically encoded proteins/enzymes provide an attractive approach target many interest, but are limited specific cell lines. Although small‐molecule‐based methods have been explored, a comprehensive system for profiling multiple locations in living cells, comparable fusion‐protein techniques, yet be established. In this study, we introduce novel proximity labeling...
African swine fever (ASF), a highly virulent viral infection, poses significant threat to the global pig industry. Currently, there are no commercially available vaccines against ASF. While crucial role of interferon (IFN) in combating infections is well-established, its impact on clinical signs and mortality rates ASF remains unclear. In this study, IFN-α2, IFN-γ, IFN-λ3 were fused with Fc segment immunoglobulin G (IgG) expressed mammalian cells (293T), antiviral efficacy detected by...
Abstract Summary Pan et al found that actomyosin-II-driven radial contractility underpins the resilience of central axons to mild mechanical stress by suppressing propagation and firing injurious Ca 2+ waves. Boosting actomyosin-II activity alleviates axon degeneration in mice with traumatic brain injury. Traumatic injury (TBI) remains a significant unmet health challenge. However, our understanding how neurons, particularly their fragile axons, withstand abrupt impacts within nervous system...
Plasmid pRST98 is a hybrid antibiotic resistance-virulence plasmid isolated fromSalmonella enterica serovar Typhi (S. Typhi). Previously, we transferred pRST98 into attenuated Salmonella Typhimurium (S. typhimurium) strain RIA to create transconjugant pRST98/RIA and indicated that pRST98 could enhance apoptosis of infected murine macrophages J774A.1. However, S. typhimurium-mice interaction cannot reflect all characteristics of S. typhi-human...