A5086940068- interferon and immune responses
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Retinoids in leukemia and cellular processes
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Liver physiology and pathology
- MicroRNA in disease regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Autophagy in Disease and Therapy
- 3D Printing in Biomedical Research
- Chronic Kidney Disease and Diabetes
- Extracellular vesicles in disease
- AI in cancer detection
- Cancer Mechanisms and Therapy
- Nerve injury and regeneration
- Neuroblastoma Research and Treatments
ShanghaiTech University
2021-2025
Community Health Center
2022-2024
Shanghai Jiao Tong University
2010-2024
Songjiang District Central Hospital
2024
Chongqing Medical University
2023
Children's Hospital of Chongqing Medical University
2023
Ruijin Hospital
2008-2022
Tongji University
1998-2021
Second Military Medical University
2013-2019
Eastern Hepatobiliary Surgery Hospital
2013-2019
Abstract Purpose: The aim of our study is to elucidate whether T cells expressing GPC3-targeted chimeric antigen receptor (CAR) can efficiently eliminate GPC3-positive HCC and their potential in the treatment HCC. Experimental Design: a first-generation third-generation CAR were prepared using lentiviral vector transduction. vitro vivo cytotoxic activities genetically engineered evaluated against various cell lines. Results: could kill but not GPC3-negative vitro. These seemed be positively...
The RIG-G gene, originally isolated from an acute promyelocytic leukemia cell line NB4, codes for a 60-kDa cytoplasmic protein that is induced by all-trans retinoic acid (ATRA) treatment along with the induction of morphological differentiation NB4 cells. Here, we provide evidence ectopic expression Rig-G in U937 cells can lead to significant accumulation at G 1 /S transition. Growth arrest seems occur modulating several major cycle regulatory players. Interestingly, alters JAB1 cellular...
This study was performed to investigate the role of galectin-1 (Gal-1) in epithelial ovarian cancer (EOC) progression and chemoresistance. Tissue samples from patients with EOC were used examine correlation between Gal-1 expression clinical stage EOC. The chemoresistance evaluated vitro by siRNA-mediated knockdown or lentivirus-mediated overexpression cell lines. To elucidate molecular mechanisms underlying Gal-1-mediated tumor chemoresistance, activities some signaling molecules associated...
Epidermal growth factor receptor (EGFR), a well-known oncogenic driver, contributes to the initiation and progression of wide range cancer types. Aberrant lipid metabolism including highly produced monounsaturated fatty acids (MUFA) is recognized as hallmark cancer. However, how EGFR regulates MUFA synthesis in remains elusive. This focus our study.The interaction between stearoyl-CoA desaturase-1 (SCD1) was detected byco-immunoprecipitation. SCD1 protein expression, stability...
Background: Clinical management of metastatic gastric cancer (mGC) remains a major challenge due to lack specific biomarkers and effective therapeutic targets. Recently, accumulating evidence has suggested that exosomes play an essential role in metastasis can be excellent reservoir novel candidate targets for cancer. Therefore, this study, we aimed reveal the proteomic profile mGC-derived exosomes. Methods: Exosomes were isolated from pooled serum samples 20 mGC patients 40 healthy controls...
Retinoic acid-induced gene G (RIG-G), a originally identified in all-trans retinoic acid-treated NB4 acute promyelocytic leukemia cells, is also induced by IFNalpha various hematopoietic and solid tumor cells. Our previous work showed that RIG-G possessed potent antiproliferative activity. However, the mechanism for transcriptional regulation of remains unknown. Here, we report signal transducer activator transcription (STAT) 2 together with IFN regulatory factor (IRF)-9 can effectively...
Colorectal carcinoma (CRC) is a major cause of cancer mortality. The aberrant expression several microRNAs associated with CRC progression; however, the molecular mechanisms underlying this phenomenon are unclear. miR-638 and SRY-box 2 (SOX2) levels were detected in 36 tumor samples their adjacent, non-tumor tissues from patients CRC, as well 4 cell lines, using real-time quantitative RT-PCR (qRT-PCR). SOX2 90 adjacent tissue immunohistochemistry. Luciferase reporter Western blot assays used...
Directed axon trafficking of mRNA via ribonucleoprotein complexes (RNPs) is essential for the proper function and survival neurons. However, mechanisms governing RNP transport in axons remain poorly understood. Here, we identify Annexin A7 (ANXA7) as a critical adaptor facilitating retrograde T-cell intracellular antigen 1 (TIA1)-containing RNPs by linking them to cytoplasmic dynein. Persistent axonal Ca2+ elevation disrupts ANXA7's linker role, causing detachment TIA1 granules from dynein,...
Background: The research on circulating tumor DNA (ctDNA) in pancreatic cancer (PC) has emerged recently. Although the detection rate of KRAS mutation ctDNA was relatively consistent with that tissue, whether mutant allele fraction (MAF) differed still not reported. So far, clinical application PC remains inconclusive. Methods: Plasma samples were collected from 110 and 52 benign (PB) disease patients. performed using droplet digital PCR compared matched tissue. We assessed utility MAFs...
Background. To investigate the promoter mutations of ACTN4 and SYNPO genes in patients with idiopathic focal segmental glomerulosclerosis (FSGS), to provide functional analysis these role FSGS occurrence. Methods. The study consisted 82 Chinese (55 had nephrotic syndrome: NS) 90 healthy individuals. Genomic DNA extracted from peripheral leukocytes individuals were used analyse gene by polymerase chain reaction (PCR) direct sequencing. Mutations matched GenBank TRANSFAC software database...
It has been found that sertraline, a widely used antidepressant drug, possessed antitumor roles in variety of cancers including liver cancer, colorectal cancer and lymphoma. In this study, we provided evidences sertraline had potent antiproliferative activity not only acute myeloid leukemia (AML) cell lines but also the fresh cells from AML patients, could induce death through both apoptosis autophagy pathways. Moreover, inhibiting pathway partially attenuate sertraline-induced growth...
Whether, to what extent, and how the axons in central nervous system (CNS) can withstand sudden mechanical impacts remain unclear. By using a microfluidic device apply controlled transverse stress axons, we determined levels that most explored their instant responses at nanoscale resolution. We found mild triggers highly reversible, rapid axon beading response, driven by actomyosin-II-dependent dynamic diameter modulations. This mechanism contributes hindering long-range spread of...
// Kesang Li 1 , Xiaorong Pan Yanyu Bi Wen Xu Cheng Chen Huiping Gao Bizhi Shi Hua Jiang Shengli Yang Liyan 2 Zonghai State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Jiaotong University School Medicine, Shanghai, China Department Pulmonary Chest Jiao Tong University, Correspondence to: Li, e-mail: Zonghaili@shsmu.edu.cn Keywords: lung squamous cell carcinoma, chimeric antigen receptor, glypican-3, CAR T Received: August 12, 2015 Accepted:...
As a validated therapeutic target in several human cancers, the EGF receptor (EGFR) provides focus to gain deeper insights into cancer pathophysiology. In this study, we report identification of naturally occurring and widely expressed EGFR isoform termed EGFRvA, which substitutes Ser/Thr-rich peptide for part carboxyl-terminal regulatory domain receptor. Intriguingly, EGFRvA expression relates more closely histopathologic grade poor prognosis patients with glioma. Ectopic cells conferred...
AC9 is one of the adenylate cyclase (AC) isoforms, which catalyze conversion ATP to cAMP, an important second messenger. We previously found that integration cAMP/PKA pathway with nuclear receptor-mediated signaling was required during all-trans retinoic acid (ATRA)-induced maturation acute promyelocytic leukemia (APL) cells. Here we showed could affect intracellular cAMP level and enhance trans-activity receptor. Knockdown in APL cell line NB4 obviously inhibit ATRA-induced differentiation....
Autophagy plays important roles in self-renewal and differentiation of stem cells. Hepatic progenitor cells (HPCs) are thought to have the ability as well possess a bipotential capacity, which allows them differentiate into both hepatocytes bile ductular However, how autophagy contributes hepatic is not understood. In this study, we use well-established rat cell lines called WB-F344, treated with 3.75 mM sodium butyrate (SB) promote WB-F344 along biliary phenotype. We found that was...
Type 2 diabetes mellitus (T2DM) is a leading risk factor for the development and progression of chronic kidney disease (CKD). However, an accurate convenient marker early detection appropriate management CKD in individuals with T2DM limited. Recent studies have demonstrated strong correlation between neutrophil-to-lymphocyte ratio (NLR) CKD. Nonetheless, predictive value NLR renal damage type diabetic patients remains understudied.
Recently, de4 EGFR, a variant of epidermal growth factor receptor (EGFR) with exon 4 deletion, was identified in glioblastoma and ovarian cancer. However, its biological function on cancer is still not clear. In this study, the expression profile EGFR contribution to epithelial cells proliferation, invasiveness drug resistance were studied. Our results showed that 48.6% (35/72) tissues had ratio positively correlated clinical stages. Compared transfectants, transfectants exhibited...