Rubén S. Rosales

ORCID: 0000-0003-3706-0181
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About
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Research Areas
  • Microbial infections and disease research
  • Aquaculture disease management and microbiota
  • Bacteriophages and microbial interactions
  • Animal Virus Infections Studies
  • Herpesvirus Infections and Treatments
  • Blood groups and transfusion
  • Educational Games and Gamification
  • Animal Disease Management and Epidemiology
  • Reproductive tract infections research
  • Mycobacterium research and diagnosis
  • T-cell and B-cell Immunology
  • E-Learning and Knowledge Management
  • Veterinary Practice and Education Studies
  • Veterinary medicine and infectious diseases
  • Antibiotic Resistance in Bacteria
  • Educational Innovations and Technology
  • Bird parasitology and diseases
  • Diverse Educational Innovations Studies
  • Genomics and Phylogenetic Studies
  • Viral Infections and Vectors
  • Plant Pathogenic Bacteria Studies
  • Xenotransplantation and immune response
  • Streptococcal Infections and Treatments
  • Knowledge Societies in the 21st Century
  • Human-Animal Interaction Studies

Universidad de Las Palmas de Gran Canaria
2005-2025

Animal and Plant Health Agency
2011-2017

National Veterinary Research Institute
2015

Kagoshima University
1996

A retrospective study of microbiological laboratory results from 2020 to 2022, obtained a veterinary diagnostic the island Gran Canaria, Spain, focused on canine otitis cases, was performed. The objective this analyze pathogen distribution, antimicrobial susceptibility, prevalence multidrug resistant phenotypes and role coinfections in cases order provide up-to-date evidence that could support effective control strategies for prevalent pathology. total 604 submissions were processed...

10.3390/ani14050742 article EN cc-by Animals 2024-02-27

ABSTRACT Mycoplasma bovis isolates with decreased susceptibilities to tetracyclines are increasingly reported worldwide. The acquired molecular mechanisms associated this phenomenon were investigated in 70 clinical of M. . Sequence analysis the two 16S rRNA-encoding genes ( rrs3 and rrs4 alleles) containing primary binding pocket for tetracycline (Tet-1 site) was performed on hydrochloride MICs 0.125 16 μg/ml. Mutations at positions A965T, A967T/C Escherichia coli numbering) helix 31, U1199C...

10.1128/aac.03876-14 article EN Antimicrobial Agents and Chemotherapy 2014-11-18

ABSTRACT Mycoplasma bovis is a major bovine pathogen associated with respiratory disease complex and responsible for substantial economic losses worldwide. M. also other clinical presentations in cattle, including mastitis, otitis, arthritis, reproductive disorders. To gain better understanding of the genetic diversity this pathogen, multilocus sequence typing (MLST) scheme was developed applied to characterization 137 isolates from diverse geographical origins, obtained healthy or...

10.1128/jcm.01910-14 article EN Journal of Clinical Microbiology 2014-12-25

The aim of this study was to investigate the presence Mycoplasma spp. and their identification in seals from Antarctica. During January February 2010, 59 Antarctic fur (Arctocephalus gazella), 17 Weddell (Leptonychotes weddellii), 5 Southern elephant (Mirounga leonina) were captured three islands. Oral genital samples collected, cultured, cloned. Intergenic Spacer Region 16S-23S rDNA (ISR) PCR products sent for sequencing. Seventy-four (91.4%) out eighty-one sampled positive. From those, 57...

10.3390/ani15070937 article EN cc-by Animals 2025-03-25

Mycoplasma hyopneumoniae (Mhy) is the causative agent of enzootic pneumonia, characterized by high morbidity and low mortality rates in intensive swine production systems. To better understand mechanisms underlying protection an inactivated whole cell vaccine, we investigated immunohistochemical differences cytokine expression vaccinated non-vaccinated pigs experimentally infected with Mhy. Four-week-old Mhy-negative (n = 24) were allocated to negative control 8), or one two Mhy-infected...

10.3390/ani13030520 article EN cc-by Animals 2023-02-01

Abstract The aim of the study was to evaluate presence Mycoplasma bovis infection and co-infections with other spp. infections in cattle. tested population one eastern region Poland containing 66 dairy cows 23 calves showing different clinical signs suffering from pneumonia, mastitis, arthritis. incidence M. examined using serological traditional mycoplasma culture methods, molecular methods - PCR polymerase chain reaction/denaturing gradient gel electrophoresis (PCR/DGGE). PCR/DGGE method...

10.1515/bvip-2015-0049 article EN Bulletin of the Veterinary Institute in Pulawy 2015-09-01

Abstract Background Mycoplasma ( M. ) hyopneumoniae , hyorhinis and hyosynoviae are significant pathogens for the porcine industry worldwide. The aim of present study was to determine antimicrobial susceptibility six key antimicrobials (tylosin, tilmicosin, tylvalosin, lincomycin, tiamulin valnemulin) routinely used treating infections caused by these pathogens. Twenty-seven 48 40 field strains isolated from clinical samples different Southern European countries between 2013 2018 using broth...

10.1186/s12917-020-02512-2 article EN cc-by BMC Veterinary Research 2020-09-03

To isolate and characterize strains of Mycoplasma agalactiae from bulk tank silo ewes' milk.Thirteen mycoplasma isolates were obtained samples sheep milk taken large silos identified as Myc. by PCR-DGGE. The typed pulsed field gel electrophoresis (PFGE), SDS-PAGE immunoblot. in vitro activity 13 antimicrobials veterinary interest was tested against these isolates. Results showed that the most effective compounds clindamycin, an antibiotic not previously described a suitable contagious...

10.1111/jam.12176 article EN Journal of Applied Microbiology 2013-02-27

Porcine reproductive and respiratory syndrome virus (PRRSV) induces a weak immune response enabling it to persist in different organs of infected pigs. This has been attributed the ability PRRSV influence induction cytokine responses. In this study, we investigated transcriptional profiles compartments mediastinal lymph node pigs with three genotype 1 strains differing pathogenicity: low virulence prototype Lelystad (LV), UK field strain 215–06 highly virulent subtype 3 SU1-Bel isolate from...

10.1186/s13567-015-0161-8 article EN cc-by Veterinary Research 2015-03-18

Mycoplasma hyorhinis impacts swine health and production in many countries, either as a primary pathogen or component of polymicrobial infection. Isolates this species are also common contaminants tissue culture lines. The genome sequence the cell isolate M. GDL-1 is presented herein.

10.1128/jb.00033-12 article EN Journal of Bacteriology 2012-03-09

Six strains with the typical characteristics of mycoplasmas were isolated from tracheae six Canarian Egyptian vultures (Neophron percnopterus majorensis). The results biochemical, serological and molecular genetic studies showed that isolates nearly identical they could be considered as representing a novel species genus Mycoplasma. Colonies possessed fried-egg appearance electron micrographs revealed pleomorphic cellular morphology lack cell wall. hydrolysed arginine required sterol for...

10.1099/ijs.0.033803-0 article EN INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 2011-07-30

Cetaceans greatly depend on their hearing system to perform many vital activities. The spiral ganglion is an essential component of the auditory pathway and can even be associated with injuries caused by anthropogenic noise. However, its anatomical location, characterized surrounding bony structures, makes anatomopathological study a difficult task. In order obtain high-quality tissue samples, perfect balance between decalcification preservation neural components must achieved. this study,...

10.3390/ani10040683 article EN cc-by Animals 2020-04-14

Aim: In this study, flow cytometry was evaluated for the determination of minimal inhibitory concentrations (MICs) nine antibacterial agents (enrofloxacin, ciprofloxacin, oxytetracycline, chloramphenicol, tylosin, lincomycin, gentamycin, spectinomycin and streptomycin) against M. hyopneumoniae. Methods Results: Flow able to detect Mycoplasma hyopneumoniae inhibition at 12 h postincubation, whereas results obtained by traditional method were only 48 h, when a visible change in medium had...

10.1111/j.1365-2672.2006.03170.x article EN Journal of Applied Microbiology 2006-10-06

MICs were determined for 15 antimicrobial agents against 37 Mycoplasma putrefaciens isolates. The most effective drug classes the fluoroquinolones, tetracyclines, lincosamide lincomycin, and macrolides. susceptibility profile of isolates correlated with geographic origin. This is first report decreased to macrolides, tetracyclines in M. strains.

10.1128/aac.00420-07 article EN Antimicrobial Agents and Chemotherapy 2007-07-17

Due to their predatory habits, raptors may serve as indicators of the presence antimicrobial-resistant bacteria in environment, but they also represent a public health risk for livestock and humans because can act reservoirs, sources spreaders these bacteria. Our objective was determine cloacal samples Canarian Egyptian vultures (Neophron percnopterus majorensis), an endemic bird prey. One hundred forty-two swabs were obtained; Escherichia coli isolated from 80.28% Salmonella 6.3% samples....

10.3390/ani10060970 article EN cc-by Animals 2020-06-03
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