A5005122608- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Prenatal Screening and Diagnostics
- Gestational Trophoblastic Disease Studies
- Epigenetics and DNA Methylation
- Pregnancy and preeclampsia studies
- RNA modifications and cancer
- Neuropeptides and Animal Physiology
- MicroRNA in disease regulation
- PI3K/AKT/mTOR signaling in cancer
- Neurotransmitter Receptor Influence on Behavior
- Circular RNAs in diseases
- Fibroblast Growth Factor Research
- Proteoglycans and glycosaminoglycans research
- Nicotinic Acetylcholine Receptors Study
- RNA Research and Splicing
- Cervical Cancer and HPV Research
- Cancer-related molecular mechanisms research
- X-ray Diffraction in Crystallography
- Reproductive System and Pregnancy
- Parkinson's Disease Mechanisms and Treatments
- Kruppel-like factors research
- Gene expression and cancer classification
- Neuroscience and Neuropharmacology Research
- Cancer-related Molecular Pathways
Second Affiliated Hospital of Zhengzhou University
2016-2024
Zhengzhou University
2015-2023
Washington University in St. Louis
2011-2014
Mallinckrodt (United States)
2010-2011
Second Affiliated Hospital of Zhejiang University
2004
Women's Hospital, School of Medicine, Zhejiang University
2003-2004
Background: DNA methylation modification has been proved to influence the phenotype of polycystic ovary syndrome (PCOS). Genome-wide association studies (GWAS) demonstrate that yes-associated protein (YAP1) genetic sites are associated with PCOS. The study aims detect status YAP1 promoter in granulosa cells (GCs) PCOS patients and explore novel therapeutic targets for Methods: Randomized controlled trial was applied a total 72 women were included study, including 36 cases health controls....
Aberrant expression of heparanase (Hpa) is associated with apoor prognosis in ovarian and cervical cancer patients. Inhibitors Hpa can prevent the growth metastasis malignant tumor cells, suramin may be such a compound that has strong anti-proliferative effects on several kinds cells. We have therefore tested whether inhibiting effect cells due to downregulation expression.Suramin at 300-600 μg/ml significantly inhibited HO-8910 PM HeLa cell 24 h, both time-dependent dose-dependent manner,...
Abstract Background Tumor necrosis factor-α (TNF-α) immunotherapy controls the progression of human cervical cancer. Here, we explored detailed molecular mechanisms played by melatonin in cancer (HeLa cells) death presence TNF-α injury, with a particular attention to mitochondrial homeostasis. Methods HeLa cells were incubated TNFα and then cell was determined via MTT assay, TUNEL staining, caspase ELISA assay western blotting. Mitochondrial function detected analyzing membrane potential...
A series of fluorine-containing N-(2-methoxyphenyl)piperazine and N-(2-fluoroethoxy)piperazine analogues were synthesized, their affinities for human dopamine D2, D3, D4 receptors determined. Radioligand binding studies identified five compounds, 18a, 20a, 20c, 20e, 21e, which bind with high affinity at D3 (Ki = 0.17−5 nM) moderate to selectivity vs D2 (ranging from ∼25- 163-fold). These compounds also evaluated intrinsic activity using a forskolin-dependent adenylyl cyclase assay. This...
To identify suitable lipophilic compounds having high potency and selectivity for vesicular acetylcholine transporter (VAChT), a heteroaromatic ring or phenyl group was introduced into the carbonyl-containing scaffold VAChT ligands. Twenty new with ALogD values between 0.53 3.2 were synthesized, their in vitro binding affinities assayed. Six of them (19a, 19e, 19g, 19k, 24a–b) displayed affinity (Ki = 0.93–18 nM racemates) moderate to over σ1 σ2 receptors 44–4400-fold). These have methyl...
Ovarian cancer remains as one of the most threatening malignancies for females in world. This study investigated pivotal role miR-940 progression ovarian and to reveal possible molecular mechanism its action. OVCAR3 cells were transfected with vector, inhibitor, and/or small interfering RNA (siRNA) targeting PKC- (si-PKC-), respectively. After transfection, cell viability apoptosis analyzed, well proliferation apoptosis-related protein expression. Compared control, upregulation suppressed...
To identify the ligands for σ1 receptors that are potent and selective, analogues of prezamicol trozamicol scaffolds carbonyl-containing vesicular acetylcholine transporter (VAChT) inhibitors were explored. Of 23 synthesized tested, 5 displayed very high affinity (Ki = 0.48–4.05 nM) selectivity relative to σ2 (σ1/σ2 >749-fold). Four five compounds (14a, 14b, 14c, 14e) showed low VAChT > 290 nM), fifth compound (14g) moderate 44.2 nM). The...
The therapeutic challenge of advanced, recurrent, and refractory cervical cancer (CC) needs to develop new molecularly targeted drugs. Rad21 is an important regulatory gene that maintains the correct dissociation sister chromatids during cell mitosis. aim this study was investigate effect on CC. expression in CC intraepithelial neoplasia III significantly increased. Women with rs2289937 C genotype (CC+CT) rs4570 rs4579555 genotypes haplotype 1 (TTTCAGGCGC) were associated risk, while women...
In the present study, differentially expressed microRNAs (miRNAs) in peritoneal exosomes that were isolated from 10 patients with epithelial ovarian cancer (EOC) metastasis abdominal cavity and participants without (NC) identified. These miRNAs revealed by next-generation sequencing categorized Gene Ontology enrichment Kyoto Encyclopedia of Genes Genomes pathway analysis their target genes. Notably, two associated EOC-miR-149-3p miR-222-5p-were There significant differences expression...
Heparanase (HPA) is an enzyme that plays important role in cancer metastasis and angiogenesis a potential target for molecular treatment of tumors. We previously found abnormally high HPA expression cervical tissues associated with poor survival increased lymph node metastasis. The present study was conducted to assess the utility inhibiting activity treatment. Two series 13 novel inhibitors were synthesized optimized. All tested reduced (IC50 values ranged from 4.47 μM 47.19 μM) inhibited...
Sigma non-opioid intracellular receptor 1 (sigma-1 receptor), a transmembrane protein, is located on cellular mitochondrial membranes and endoplasmic reticulum. Current research has demonstrated that sigma-1 related to human degenerative diseases. This study focused the effects of pathophysiological process diminished ovarian reserve (DOR) granulosa cells (GCs) apoptosis. Sigma-1 concentration in follicular fluid (FF) serum were negatively correlated with basal follicle-stimulating hormone...