A5025569576- Legionella and Acanthamoeba research
- Parasitic Diseases Research and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Yersinia bacterium, plague, ectoparasites research
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Phytochemistry and Biological Activities
- Parasitic Infections and Diagnostics
- Bacterial biofilms and quorum sensing
- Glutathione Transferases and Polymorphisms
- Natural product bioactivities and synthesis
- Genomics, phytochemicals, and oxidative stress
- Amoebic Infections and Treatments
- Nematode management and characterization studies
- Parasitic infections in humans and animals
- Insect Pest Control Strategies
- Toxin Mechanisms and Immunotoxins
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Travel-related health issues
- Helminth infection and control
- Essential Oils and Antimicrobial Activity
- Methemoglobinemia and Tumor Lysis Syndrome
- Fecal contamination and water quality
- Medicinal Plant Extracts Effects
Poznan University of Medical Sciences
2014-2023
Three species from the Eryngium L. genus—E. campestre, E. maritimum, and planum, plants with a rich chemical composition, were selected for phytochemical biological studies. The applied biotechnological methods allowed to obtain biomass of these rare or protected in form multiplied shoots (stationary system) roots cultured liquid medium (agitated system). In extracts raw material obtained under vitro conditions, content phenolic acids flavonoids (HPLC-DAD method) as well total polyphenols...
The treatment of acanthamoebiasis is a great problem. Most cerebral invasions end with death, and the ocular usually long-lasting not very effective. Numerous plant extracts substances isolated from plants, which are effective against trophozoites or cysts, have been studied in acanthamoebiasis. However, no agents that simultaneously both developing forms amoebae discovered yet. It seems such fulfils tasks Artemisia annua L. Our studies showed water, alcohol chloroform herb A. can be applied...
The free-living Acanthamoeba sp.causes various diseases.Treatment of them is very difficult and not always effective because encystation, making it highly resistant to antiamoebic drugs.Gram-positive bacteria staphylococcus aureus, Gram-negative Escherichia coli, an yeast candida albicans also exhibit outstanding resistance antimicrobial substances.The search for new natural amoebicidal agents plant origin still current interest.The aim the study was investigate activity extracts obtained...
Alveolar echinococcosis (AE) is a chronic zoonotic disease caused by the larval form of Echinococcus multilocularis. In humans, it may become serious infection liver which resembles slow malignant process leading to death when untreated. The aim study was an assessment risk factors E. multilocularis infections and description AE clinical course in group 36 patients with confirmed AE, hospitalized at Department Clinic Tropical Parasitic Diseases, Poznan University Medical Sciences between...
Genetically uniform plant material, derived from Lychnis flos-cuculi propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B subjected to an evaluation antifungal antiamoebic activity. The activity 80% aqueous methanolic extracts, their fractions, isolated ecdysteroids were studied against pathogenic Acanthamoeba castellani. Additionally, a Microtox® acute toxicity assay performed. It found that fraction root extract exerts most potent amoebicidal at IC50 0.06...
The Toll-like receptors (TLRs) of the innate immune system play an important role in recognition pathogens such as bacteria, viruses, fungi, and parasites. In this study, we examined changes level expression TLR2 TLR4 mRNA protein brains mice infected with Acanthamoeba spp. strains were isolated from a patient keratitis (AK) (Ac55) Malta Lake (Ac43). brain at 2 days post-infection (dpi) Ac55 Ac43, mRNAs for significantly more strongly expressed comparison uninfected mice....
Genetically uniform shoots of Eryngium alpinum L. cultured in vitro were subjected to the qualitative analysis applying UPLC-HESI-HRMS technique. In cultures give opportunity perform phytochemical studies on protected species without harvesting plant material from natural environment. The screening crude methanolic extracts shoots, both and vivo plants, revealed presence phenolic acids, coumarins, flavonoids, triterpenoid saponins, amino or dipeptides. Active compounds detected are known...
The glutathione S-transferases (GSTs) are a family of multifunctional enzymes involved in cellular detoxification. aim this study was to evaluate the effect albendazole--drug choice for trichinellosis--on total activity and kinetics cytosolic GST mouse intestines during experimental trichinellosis. Our results showed statistically significant decrease both small large mice infected with nematode Trichinella spiralis (Owen, 1835) treated albendazole, compared control that were but untreated...
Free-living amoebae belonging to the genus Acanthamoeba are causative factor of many diseases. Among others, they cause keratitis (AK), a condition that usually occurs in contact lens wearers, though it is also observed non-wearers. The number diagnosed cases AK increased more than eightfold during 8 years USA, and proportional increase frequency occurred Poland Europe. Cases late, their therapy difficult rarely successful. an uncommon diagnosis Poland. positive our laboratory may reflect...
Some protozoans are able to encyst as a protective response harmful environment. The cyst wall usually contains chitin its main structural constituent. Acanthamoeba is an exception since cellulose. Specific cytochemical differentiation between cellulose and by microscopy has not been possible due the similarity of constituent beta-1,4-linked hexose backbones these molecules. Thus, various fluorescent brightening agents lectins bind both chitin. identification spp., which based primarily on...
Acanthamoeba are widespread free-living amoebae which may cause granulomatous amoebic encephalitis (GAE), keratitis, skin ulcerations and disseminated tissue infection. An important diagnostic prognostic factor for the treatment of infection is a quick correct diagnosis strains. The aim our study was to develop rapid method detection identification pathogenic spp. strains from material collected water. In this we analysed five amplification-based genetic markers (Aca 16S, Ac6/210, GP, JDP,...